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The map label for this gene is pdhC [H]

Identifier: 55980201

GI number: 55980201

Start: 224493

End: 225848

Strand: Direct

Name: pdhC [H]

Synonym: TTHA0232

Alternate gene names: 55980201

Gene position: 224493-225848 (Clockwise)

Preceding gene: 55980200

Following gene: 55980202

Centisome position: 12.14

GC content: 67.92

Gene sequence:

>1356_bases
ATGCCCAAGGAAATCCTCATGCCCGAGCTCGCCGAAAGCGTGGTGGAAGGCGAGATTCTGAAGTGGCTCGTGGAGGAAGG
GGACTACCTGAAGAAGGACCAGCCCTTCGTGGAGGTCATGACCGACAAGGTTACGGTGGAGCTGCCCTCCCCTTACGAGG
GGGTGCTCCTGAAGAAGCTGGCCAAGGAGGGGGAGGTGGTCAAGGTCCACGCCCCCATCGCCCTCATCGCCGAGCCCGGC
GAGGCGGTGGAGGGCGTGAAGGAGGCCCCCCCGGTGCAGGCGGTGGAGGAGCGCTCCATCGTGGAGCCGGGCCTGCCCGC
GAAGGAGGAGAAGGAGGACCTCTCCCTCTTCAAGCCGGACCCCACGCAGGTGGCGGTGAAAAACCCCTTCCTCTCCGGGG
AAAAGCCCCAGGAGGGGGCGAGGCCGGGGCGGGTCCTCGCCGTCCCCGCCGCCCGGAAGCTCGCCCGGGAGCTCGGGATC
CCCATTGAGGAGGTGCCGGGCTCGGGGCCTTTGGGCAGGGTCCGGGTGGAGGACGTCCGGGCCTACGCCGAGCGGAGGAA
GGCCCCTCCCGAAAGGCCCGAGGAGGGGCCCCAGGTGCTTCCTGCCGGCTTCCCGCCCCCGCCCAAGTACGCCCCGCCCA
AGGGGTACGAGCACCTGGAGGAGCGGGTGCCTCTAAGGGGCATCCGCCGCACCATCGCCCAGGGGCTCTGGCAGAGCCAC
CTCTACACGGTGCGCACCCTCAACGTGGACGAGGCCGACCTCACGGAGCTCGTGCGCCTGCGCGAGCGCCTCAAGCCCCA
GGCCGAGGCCCAGGGGGTCAAGCTCACCTACCTGCCCTTCATCGTCAAGGCAGTGGTGCGGGCCCTGAAGAAGTTCCCCA
TGCTGAACACCAGCCTGGACGAGGAGAGGCAGGAGATCGTCTACAAGCGCTACTACCACATCGGCCTCGCCGTGGCCACG
GAAAGGGGCCTCATCGTCCCCGTGGTGCGGGATGCGGACCGGAAGAGCGTCTTGGAGCTCGCCCAGGAGATCGCCGAGCT
CTCCCAGAAGGCGCGGGAAGGCCGCCTCGCCCCGGAGGAGGTTACGGGGTCCACCTTCACCATCACCAACATCGGCTCCG
TGGGGGCCACCTTGAGCTTCCCCATCATCCACCTGCCGGACGCGGCCATCCTCGGCGTGCACTCCATAAGGAAGCGCCCC
TGGGTCATGCCGGACGGCTCCATAAGGCCGCGGGACATCATGTTCCTCTCCCTCTCCTTTGACCACCGCCTGGTGGACGG
GGCCGAGGCGGCCATGTTCACCCGGGAGGTCATCAGGCTCTTGGAAAACCCCGACCTGCTCCTTCTGGAAATGTAG

Upstream 100 bases:

>100_bases
AAGGTGGGAACCTTGGCCGGAATCCTAAAGGCGGTGGCGGAAGCCCGTGGGATAGACCGCAAGGAACTTCAACAGCTCCT
GGACCTGTAAAGGGAGGACT

Downstream 100 bases:

>100_bases
GATGACGCCTATGAAGACCTACGACCTCATCGTGATCGGCACCGGCCCGGGGGGCTACCACGCCGCCATCCGGGCCGCCC
AGCTCGGCCTCAAGGTGCTG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MPKEILMPELAESVVEGEILKWLVEEGDYLKKDQPFVEVMTDKVTVELPSPYEGVLLKKLAKEGEVVKVHAPIALIAEPG
EAVEGVKEAPPVQAVEERSIVEPGLPAKEEKEDLSLFKPDPTQVAVKNPFLSGEKPQEGARPGRVLAVPAARKLARELGI
PIEEVPGSGPLGRVRVEDVRAYAERRKAPPERPEEGPQVLPAGFPPPPKYAPPKGYEHLEERVPLRGIRRTIAQGLWQSH
LYTVRTLNVDEADLTELVRLRERLKPQAEAQGVKLTYLPFIVKAVVRALKKFPMLNTSLDEERQEIVYKRYYHIGLAVAT
ERGLIVPVVRDADRKSVLELAQEIAELSQKAREGRLAPEEVTGSTFTITNIGSVGATLSFPIIHLPDAAILGVHSIRKRP
WVMPDGSIRPRDIMFLSLSFDHRLVDGAEAAMFTREVIRLLENPDLLLLEM

Sequences:

>Translated_451_residues
MPKEILMPELAESVVEGEILKWLVEEGDYLKKDQPFVEVMTDKVTVELPSPYEGVLLKKLAKEGEVVKVHAPIALIAEPG
EAVEGVKEAPPVQAVEERSIVEPGLPAKEEKEDLSLFKPDPTQVAVKNPFLSGEKPQEGARPGRVLAVPAARKLARELGI
PIEEVPGSGPLGRVRVEDVRAYAERRKAPPERPEEGPQVLPAGFPPPPKYAPPKGYEHLEERVPLRGIRRTIAQGLWQSH
LYTVRTLNVDEADLTELVRLRERLKPQAEAQGVKLTYLPFIVKAVVRALKKFPMLNTSLDEERQEIVYKRYYHIGLAVAT
ERGLIVPVVRDADRKSVLELAQEIAELSQKAREGRLAPEEVTGSTFTITNIGSVGATLSFPIIHLPDAAILGVHSIRKRP
WVMPDGSIRPRDIMFLSLSFDHRLVDGAEAAMFTREVIRLLENPDLLLLEM
>Mature_450_residues
PKEILMPELAESVVEGEILKWLVEEGDYLKKDQPFVEVMTDKVTVELPSPYEGVLLKKLAKEGEVVKVHAPIALIAEPGE
AVEGVKEAPPVQAVEERSIVEPGLPAKEEKEDLSLFKPDPTQVAVKNPFLSGEKPQEGARPGRVLAVPAARKLARELGIP
IEEVPGSGPLGRVRVEDVRAYAERRKAPPERPEEGPQVLPAGFPPPPKYAPPKGYEHLEERVPLRGIRRTIAQGLWQSHL
YTVRTLNVDEADLTELVRLRERLKPQAEAQGVKLTYLPFIVKAVVRALKKFPMLNTSLDEERQEIVYKRYYHIGLAVATE
RGLIVPVVRDADRKSVLELAQEIAELSQKAREGRLAPEEVTGSTFTITNIGSVGATLSFPIIHLPDAAILGVHSIRKRPW
VMPDGSIRPRDIMFLSLSFDHRLVDGAEAAMFTREVIRLLENPDLLLLEM

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=32.1507760532151, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=36.6141732283465, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI31711992, Length=461, Percent_Identity=30.58568329718, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI203098816, Length=467, Percent_Identity=27.6231263383298, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI203098753, Length=467, Percent_Identity=27.6231263383298, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI260898739, Length=165, Percent_Identity=32.7272727272727, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786946, Length=449, Percent_Identity=31.8485523385301, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1786305, Length=310, Percent_Identity=37.4193548387097, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=448, Percent_Identity=30.3571428571429, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI25146366, Length=449, Percent_Identity=31.8485523385301, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17560088, Length=470, Percent_Identity=29.5744680851064, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=318, Percent_Identity=29.2452830188679, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=447, Percent_Identity=31.9910514541387, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=27.4944567627494, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI18859875, Length=454, Percent_Identity=29.0748898678414, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=33.9130434782609, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24582497, Length=316, Percent_Identity=29.1139240506329, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI20129315, Length=316, Percent_Identity=29.1139240506329, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 50180; Mature: 50049

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEILMPELAESVVEGEILKWLVEEGDYLKKDQPFVEVMTDKVTVELPSPYEGVLLKKL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECEEEEECCCCHHHHHHHHH
AKEGEVVKVHAPIALIAEPGEAVEGVKEAPPVQAVEERSIVEPGLPAKEEKEDLSLFKPD
HCCCCEEEEECCEEEEECCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PTQVAVKNPFLSGEKPQEGARPGRVLAVPAARKLARELGIPIEEVPGSGPLGRVRVEDVR
CCEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCHHHCCCCCCCCCEEHHHHH
AYAERRKAPPERPEEGPQVLPAGFPPPPKYAPPKGYEHLEERVPLRGIRRTIAQGLWQSH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
LYTVRTLNVDEADLTELVRLRERLKPQAEAQGVKLTYLPFIVKAVVRALKKFPMLNTSLD
CEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCHH
EERQEIVYKRYYHIGLAVATERGLIVPVVRDADRKSVLELAQEIAELSQKAREGRLAPEE
HHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VTGSTFTITNIGSVGATLSFPIIHLPDAAILGVHSIRKRPWVMPDGSIRPRDIMFLSLSF
HCCCEEEEEECCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECC
DHRLVDGAEAAMFTREVIRLLENPDLLLLEM
CCHHHCCHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
PKEILMPELAESVVEGEILKWLVEEGDYLKKDQPFVEVMTDKVTVELPSPYEGVLLKKL
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECEEEEECCCCHHHHHHHHH
AKEGEVVKVHAPIALIAEPGEAVEGVKEAPPVQAVEERSIVEPGLPAKEEKEDLSLFKPD
HCCCCEEEEECCEEEEECCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PTQVAVKNPFLSGEKPQEGARPGRVLAVPAARKLARELGIPIEEVPGSGPLGRVRVEDVR
CCEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCHHHCCCCCCCCCEEHHHHH
AYAERRKAPPERPEEGPQVLPAGFPPPPKYAPPKGYEHLEERVPLRGIRRTIAQGLWQSH
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
LYTVRTLNVDEADLTELVRLRERLKPQAEAQGVKLTYLPFIVKAVVRALKKFPMLNTSLD
CEEEEEECCCHHHHHHHHHHHHHCCCHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCHH
EERQEIVYKRYYHIGLAVATERGLIVPVVRDADRKSVLELAQEIAELSQKAREGRLAPEE
HHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VTGSTFTITNIGSVGATLSFPIIHLPDAAILGVHSIRKRPWVMPDGSIRPRDIMFLSLSF
HCCCEEEEEECCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECC
DHRLVDGAEAAMFTREVIRLLENPDLLLLEM
CCHHHCCHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]