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The map label for this gene is purA

Identifier: 55980191

GI number: 55980191

Start: 216549

End: 217775

Strand: Direct

Name: purA

Synonym: TTHA0222

Alternate gene names: 55980191

Gene position: 216549-217775 (Clockwise)

Preceding gene: 55980190

Following gene: 55980193

Centisome position: 11.71

GC content: 68.95

Gene sequence:

>1227_bases
ATGCCGGGCATCGCCATCATCGGGGCGCAGTGGGGCGACGAGGGCAAGGGCAAGGTGGTGGACGTCCTGGCCCGGGAAGC
CGACTACGTGATCCGCTACCAGGGCGGGGCCAACGCCGGCCACACCGTGGTGGCCGAGGGCAAGGTCTTCAAGCTCAACC
TCCTGCCCTCGGGGGTCATCCACCCCCACGCGGTGAACGTCCTGGGGGACGGGATGGTCATTGACCCCTTCCGCTTCCAG
GAGGAGGTGGAAGGCCTGCGGAAGGAGGGGTTTGACCCCAAGATCCTGGTCTCGGAAAGGGCCCACCTCGTCCTCCCCCA
CCACAAGCACGTGGAAAGCCGCCACAACTTCGTGGGCACCACGGGGCGCGGCATCGGTCCCGCCTACTCCGACCGGGCAA
GAAGGGTGGGGATCCGGGCTGGGGACCTTTTGGACGAGGCCACCCTGAGGGAGAGGGTGCGCCGCCTCCTCGCCGAGAAG
CCCAACTCCACCCGGGAAGCGGGCTGGGATACGGAGGAAAAGGCCCTCGCCGATCTCCACCGGATGCGGGAGATCCTAAG
CCCCTACATCGCCGACACGGGGAGCCTCCTCCGGGAGGCCTGGCGCAAGGGGAAGCGCCTCCTCTTTGAGGGGGCCCAGG
CCACCCTGCTGGACCTGAACTACGGCACCTACCCCTACGTCACGAGCTCCCATCCCACGGTGGGGGGGATCCTGGTGGGG
ACGGGCCTCTCCCACAAGGCCATCACCAAGGTCTACGGCGTGGCCAAGGCCTACACCACGAGGGTAGGGGAAGGCCCCTT
CCCCACGGAGCTCCAGGGGGAGCTCGCCCACCACCTCCGGGAAAAGGGCGGCGAGTACGGCACCACCACGGGAAGGCCCA
GGAGGGTGGGCTGGCTGGACCTGGTGGCCCTCCGGTACGCCTGCGAGGTGAACGGCTTTGACGGCCTCGTCCTCACCAAG
CTGGACGTGCTCTCCGGGCTGGAGAAGGTGAAGGTGGCCGTGGAGTACCTGGACGGGGCCCGCCCCGGGGAGGCGAGCCC
GGAGGCGGTGCGCTACTTGGAGCTTCCGGGCTGGGGCGACCTCTCCCACGTGAAGCGCCGGGAGGACCTCCCCGCAAACC
TCCTCCGCTACCTGGAGCTCGTGGAGGAGCACACCGGGGTCCCCGTGGTCCTCTTCTCCACGAGCCCCAGGCGGGAGGAC
ACCTTCGGGGCGGTGAGCTGGGTCTAA

Upstream 100 bases:

>100_bases
CTGAGCCTTGGCTTTCGCCTGGCTCCTTCATTATACCGCGGCTTGCGCACTTGTAAAGGCCTATGCGCCGCTCCCGTCCC
CGCCTGTAGACTTAGAGGGT

Downstream 100 bases:

>100_bases
GGGTCTACCCCAGACGGGAGACGCGCAGGAGGAACCTCTTCTCCACGGGGAAGGGCCGGTCCAGGTCGCCGAGCTCCGCC
TCGGCCCAGGCCCAAAGCCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPGIAIIGAQWGDEGKGKVVDVLAREADYVIRYQGGANAGHTVVAEGKVFKLNLLPSGVIHPHAVNVLGDGMVIDPFRFQ
EEVEGLRKEGFDPKILVSERAHLVLPHHKHVESRHNFVGTTGRGIGPAYSDRARRVGIRAGDLLDEATLRERVRRLLAEK
PNSTREAGWDTEEKALADLHRMREILSPYIADTGSLLREAWRKGKRLLFEGAQATLLDLNYGTYPYVTSSHPTVGGILVG
TGLSHKAITKVYGVAKAYTTRVGEGPFPTELQGELAHHLREKGGEYGTTTGRPRRVGWLDLVALRYACEVNGFDGLVLTK
LDVLSGLEKVKVAVEYLDGARPGEASPEAVRYLELPGWGDLSHVKRREDLPANLLRYLELVEEHTGVPVVLFSTSPRRED
TFGAVSWV

Sequences:

>Translated_408_residues
MPGIAIIGAQWGDEGKGKVVDVLAREADYVIRYQGGANAGHTVVAEGKVFKLNLLPSGVIHPHAVNVLGDGMVIDPFRFQ
EEVEGLRKEGFDPKILVSERAHLVLPHHKHVESRHNFVGTTGRGIGPAYSDRARRVGIRAGDLLDEATLRERVRRLLAEK
PNSTREAGWDTEEKALADLHRMREILSPYIADTGSLLREAWRKGKRLLFEGAQATLLDLNYGTYPYVTSSHPTVGGILVG
TGLSHKAITKVYGVAKAYTTRVGEGPFPTELQGELAHHLREKGGEYGTTTGRPRRVGWLDLVALRYACEVNGFDGLVLTK
LDVLSGLEKVKVAVEYLDGARPGEASPEAVRYLELPGWGDLSHVKRREDLPANLLRYLELVEEHTGVPVVLFSTSPRRED
TFGAVSWV
>Mature_407_residues
PGIAIIGAQWGDEGKGKVVDVLAREADYVIRYQGGANAGHTVVAEGKVFKLNLLPSGVIHPHAVNVLGDGMVIDPFRFQE
EVEGLRKEGFDPKILVSERAHLVLPHHKHVESRHNFVGTTGRGIGPAYSDRARRVGIRAGDLLDEATLRERVRRLLAEKP
NSTREAGWDTEEKALADLHRMREILSPYIADTGSLLREAWRKGKRLLFEGAQATLLDLNYGTYPYVTSSHPTVGGILVGT
GLSHKAITKVYGVAKAYTTRVGEGPFPTELQGELAHHLREKGGEYGTTTGRPRRVGWLDLVALRYACEVNGFDGLVLTKL
DVLSGLEKVKVAVEYLDGARPGEASPEAVRYLELPGWGDLSHVKRREDLPANLLRYLELVEEHTGVPVVLFSTSPRREDT
FGAVSWV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=417, Percent_Identity=44.8441247002398, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI34577063, Length=418, Percent_Identity=42.1052631578947, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI40316944, Length=390, Percent_Identity=42.8205128205128, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1790620, Length=421, Percent_Identity=42.5178147268409, Blast_Score=330, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI25146553, Length=420, Percent_Identity=41.9047619047619, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI25146555, Length=420, Percent_Identity=41.9047619047619, Blast_Score=299, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6324109, Length=423, Percent_Identity=41.371158392435, Blast_Score=325, Evalue=8e-90,
Organism=Drosophila melanogaster, GI21356033, Length=406, Percent_Identity=44.8275862068966, Blast_Score=313, Evalue=1e-85,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_THET2 (Q72GT4)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_005733.1
- ProteinModelPortal:   Q72GT4
- SMR:   Q72GT4
- STRING:   Q72GT4
- GeneID:   2776141
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q72GT4
- ProtClustDB:   PRK13788
- BioCyc:   TTHE262724:TT_C1764-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 44880; Mature: 44749

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 121-121 BINDING 135-135 BINDING 213-213 BINDING 228-228 BINDING 292-292 BINDING 294-294

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIAIIGAQWGDEGKGKVVDVLAREADYVIRYQGGANAGHTVVAEGKVFKLNLLPSGVI
CCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEECCEEEEEEECCCCCC
HPHAVNVLGDGMVIDPFRFQEEVEGLRKEGFDPKILVSERAHLVLPHHKHVESRHNFVGT
CCCCHHEECCCEEECHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCHHHHHHHCCCCCC
TGRGIGPAYSDRARRVGIRAGDLLDEATLRERVRRLLAEKPNSTREAGWDTEEKALADLH
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
RMREILSPYIADTGSLLREAWRKGKRLLFEGAQATLLDLNYGTYPYVTSSHPTVGGILVG
HHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCCEEECCCCCCCEEEEE
TGLSHKAITKVYGVAKAYTTRVGEGPFPTELQGELAHHLREKGGEYGTTTGRPRRVGWLD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LVALRYACEVNGFDGLVLTKLDVLSGLEKVKVAVEYLDGARPGEASPEAVRYLELPGWGD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCCC
LSHVKRREDLPANLLRYLELVEEHTGVPVVLFSTSPRREDTFGAVSWV
HHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGIAIIGAQWGDEGKGKVVDVLAREADYVIRYQGGANAGHTVVAEGKVFKLNLLPSGVI
CCEEEEECCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEECCEEEEEEECCCCCC
HPHAVNVLGDGMVIDPFRFQEEVEGLRKEGFDPKILVSERAHLVLPHHKHVESRHNFVGT
CCCCHHEECCCEEECHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCHHHHHHHCCCCCC
TGRGIGPAYSDRARRVGIRAGDLLDEATLRERVRRLLAEKPNSTREAGWDTEEKALADLH
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
RMREILSPYIADTGSLLREAWRKGKRLLFEGAQATLLDLNYGTYPYVTSSHPTVGGILVG
HHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCCEEECCCCCCCEEEEE
TGLSHKAITKVYGVAKAYTTRVGEGPFPTELQGELAHHLREKGGEYGTTTGRPRRVGWLD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LVALRYACEVNGFDGLVLTKLDVLSGLEKVKVAVEYLDGARPGEASPEAVRYLELPGWGD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCCC
LSHVKRREDLPANLLRYLELVEEHTGVPVVLFSTSPRREDTFGAVSWV
HHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA