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The map label for this gene is alaXL [H]

Identifier: 55980115

GI number: 55980115

Start: 142125

End: 143252

Strand: Reverse

Name: alaXL [H]

Synonym: TTHA0146

Alternate gene names: 55980115

Gene position: 143252-142125 (Counterclockwise)

Preceding gene: 55980118

Following gene: 55980114

Centisome position: 7.74

GC content: 70.83

Gene sequence:

>1128_bases
ATGAGGCTCTACCAGCTGGACAGCTACGCCACCCGCTTCCAGGCCCGGGTGGAGCGGGTCTGGAGCGACGAAAAGGGGCA
CTACGCGGTGCTTTCCCAGACCCTCTTCTACCCCGAGTCCGGGGGGCAGCCCGCGGACCGAGGGGTCCTCAGGGGGCCTT
TCGGCGAGGTCCAGGTCCTCCACGCCTACGAGGAGGAGAAGGCCTTCGGGGACGTGGTCCACGTGCTCTCCGCGCCCATC
CCAGAGGGCGTGGAGGTGGAGGGGGAGATTGACTGGCCGAGGCGCTTCCGCCACATGCAGCGCCACACCGCCCAGCACCT
CCTCTCCCAGGCCCTCCTCAGGGCCGGGGGCTACCACACCGTGGCCGTGAGCCTGGACTCCCCGGTGTGCACCGTGGACC
TCGAGGAGGAGGCCGAGGAAGCGGGGGTCCTCGAGGCCGAGGCCCTCGCCAACTTCGCCGTCTACGCCGACTACCCGGTG
GAGGCCTTTTACGTCTCCGAGGAGGAGCTCGCCCGCTACCCCCTGAGGCGCCCCCCCAAGGTCCGGGGCAAGGTGCGCCT
GGTGCGGATCGGCGACTTTGACCTCGCCGCCTGCGGGGGGACCCACCTCAAGACCAGCGCCCAGGCAGGGCCCATCAAGG
TCCTCAAGTGGGAGCGGTACAAGGGAGGGACGCGGGTGTACTTCATGGCCGGCTGGGAGGCCCTGGAGGACTACCACGCC
AAGCACGCCCTCCTTGCCCGCCTGGCCCTCGCCTTCTCCACGAACCCCTTGGAGCTGGAGAAGCCCATAAGGAAGCTCCA
GGACGAGCTCTACGCCCTAAAGGGGGAGAACCAGGCCCTGAGGGAGGGGCTTGCGGAGGCCCTCCTGCCCAGGGCCCTGG
AGGAGAAGGCCCTCCTCGTCCCCCTCCCCGTCCTCGGGGAGCTCGGCAAGAGGCTCGCCCGCTTTGAGGGCACCTTCCTC
CTCGTCGCCCCCGAGGGGCGGTTCGTGGCCGTGGGGCCGAGGCGGGGGGAGGTGCTGGAGCGCCTCAAGGCCCTGGGGGG
CCGGGGAGGAGGGAAGGAGGCGGTCCAGGGCGCCCTTCCCCCGGGGCGGGCGGTGGAGGCTTTGGCCCGGGTGCGGGAAC
TGCTCTGA

Upstream 100 bases:

>100_bases
CAGGTCCTGCGTCGCCGAGGCCAAGGAGCGGCGGGCCTGGGAAAACCCCCTGGCGAACTCCTCCCGGTCCAGGGGCGCCA
TCCTCCTTATCCTAAGGGGC

Downstream 100 bases:

>100_bases
CCCGGGCCAAAGCCGGCCTTTCCGGGGCCCAGAAGAAGCGCCTGACACTCCCCTGACCCGGCCCCCGCTAGACTGGGGGT
GTGGCCACCCTGCTTCTGGT

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRLYQLDSYATRFQARVERVWSDEKGHYAVLSQTLFYPESGGQPADRGVLRGPFGEVQVLHAYEEEKAFGDVVHVLSAPI
PEGVEVEGEIDWPRRFRHMQRHTAQHLLSQALLRAGGYHTVAVSLDSPVCTVDLEEEAEEAGVLEAEALANFAVYADYPV
EAFYVSEEELARYPLRRPPKVRGKVRLVRIGDFDLAACGGTHLKTSAQAGPIKVLKWERYKGGTRVYFMAGWEALEDYHA
KHALLARLALAFSTNPLELEKPIRKLQDELYALKGENQALREGLAEALLPRALEEKALLVPLPVLGELGKRLARFEGTFL
LVAPEGRFVAVGPRRGEVLERLKALGGRGGGKEAVQGALPPGRAVEALARVRELL

Sequences:

>Translated_375_residues
MRLYQLDSYATRFQARVERVWSDEKGHYAVLSQTLFYPESGGQPADRGVLRGPFGEVQVLHAYEEEKAFGDVVHVLSAPI
PEGVEVEGEIDWPRRFRHMQRHTAQHLLSQALLRAGGYHTVAVSLDSPVCTVDLEEEAEEAGVLEAEALANFAVYADYPV
EAFYVSEEELARYPLRRPPKVRGKVRLVRIGDFDLAACGGTHLKTSAQAGPIKVLKWERYKGGTRVYFMAGWEALEDYHA
KHALLARLALAFSTNPLELEKPIRKLQDELYALKGENQALREGLAEALLPRALEEKALLVPLPVLGELGKRLARFEGTFL
LVAPEGRFVAVGPRRGEVLERLKALGGRGGGKEAVQGALPPGRAVEALARVRELL
>Mature_375_residues
MRLYQLDSYATRFQARVERVWSDEKGHYAVLSQTLFYPESGGQPADRGVLRGPFGEVQVLHAYEEEKAFGDVVHVLSAPI
PEGVEVEGEIDWPRRFRHMQRHTAQHLLSQALLRAGGYHTVAVSLDSPVCTVDLEEEAEEAGVLEAEALANFAVYADYPV
EAFYVSEEELARYPLRRPPKVRGKVRLVRIGDFDLAACGGTHLKTSAQAGPIKVLKWERYKGGTRVYFMAGWEALEDYHA
KHALLARLALAFSTNPLELEKPIRKLQDELYALKGENQALREGLAEALLPRALEEKALLVPLPVLGELGKRLARFEGTFL
LVAPEGRFVAVGPRRGEVLERLKALGGRGGGKEAVQGALPPGRAVEALARVRELL

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=392, Percent_Identity=26.2755102040816, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI217416402, Length=392, Percent_Identity=26.2755102040816, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789048, Length=355, Percent_Identity=24.5070422535211, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18543305, Length=429, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 41575; Mature: 41575

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYQLDSYATRFQARVERVWSDEKGHYAVLSQTLFYPESGGQPADRGVLRGPFGEVQVL
CEEEECHHHHHHHHHHHHHHHCCCCCCEEEEHHEEECCCCCCCCCCCCCCCCCCCCEEEE
HAYEEEKAFGDVVHVLSAPIPEGVEVEGEIDWPRRFRHMQRHTAQHLLSQALLRAGGYHT
EHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VAVSLDSPVCTVDLEEEAEEAGVLEAEALANFAVYADYPVEAFYVSEEELARYPLRRPPK
EEEEECCCEEEEECHHHHHHCCCEEHHHHHCEEEEECCCCEEEEECHHHHHHCCCCCCCC
VRGKVRLVRIGDFDLAACGGTHLKTSAQAGPIKVLKWERYKGGTRVYFMAGWEALEDYHA
CCCCEEEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECHHHHHHHHH
KHALLARLALAFSTNPLELEKPIRKLQDELYALKGENQALREGLAEALLPRALEEKALLV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEE
PLPVLGELGKRLARFEGTFLLVAPEGRFVAVGPRRGEVLERLKALGGRGGGKEAVQGALP
CCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCC
PGRAVEALARVRELL
CCHHHHHHHHHHHHC
>Mature Secondary Structure
MRLYQLDSYATRFQARVERVWSDEKGHYAVLSQTLFYPESGGQPADRGVLRGPFGEVQVL
CEEEECHHHHHHHHHHHHHHHCCCCCCEEEEHHEEECCCCCCCCCCCCCCCCCCCCEEEE
HAYEEEKAFGDVVHVLSAPIPEGVEVEGEIDWPRRFRHMQRHTAQHLLSQALLRAGGYHT
EHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VAVSLDSPVCTVDLEEEAEEAGVLEAEALANFAVYADYPVEAFYVSEEELARYPLRRPPK
EEEEECCCEEEEECHHHHHHCCCEEHHHHHCEEEEECCCCEEEEECHHHHHHCCCCCCCC
VRGKVRLVRIGDFDLAACGGTHLKTSAQAGPIKVLKWERYKGGTRVYFMAGWEALEDYHA
CCCCEEEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECHHHHHHHHH
KHALLARLALAFSTNPLELEKPIRKLQDELYALKGENQALREGLAEALLPRALEEKALLV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEE
PLPVLGELGKRLARFEGTFLLVAPEGRFVAVGPRRGEVLERLKALGGRGGGKEAVQGALP
CCHHHHHHHHHHHHHCCCEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCHHHHHCCCC
PGRAVEALARVRELL
CCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]