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The map label for this gene is murE [H]

Identifier: 55980069

GI number: 55980069

Start: 100538

End: 101986

Strand: Direct

Name: murE [H]

Synonym: TTHA0100

Alternate gene names: 55980069

Gene position: 100538-101986 (Clockwise)

Preceding gene: 55980068

Following gene: 55980074

Centisome position: 5.44

GC content: 73.57

Gene sequence:

>1449_bases
ATGCCCACCTTGAAGGAGCTCTTCGCCCTCTTCGGGAAGGAAGCCCCGGAGGTGCCCGTCAGGGGCCTCACCCTGGACTC
GAGGCGGGTGGAGCCCGGCTTCGTCTTCGTGGCCGTACCCGGGGTTCCCCTCCCCCACCGGAAGCCCCTGGACGGGCACG
ACTTCGTCCCCGAGGCCCTGGCCCGGGGGGCGGTGGCCGTGGTGGGGGAAAGGCCCCTCGCCCTCCCCGTCCCCTACCTC
CAGGTGGCCGACGGCCGCCAGGCCCTCGCCCTCCTGGCCCGGCGCTTCCACGGGGAGCCGGACCGGAAGCTCCTCCTCGT
GGGGGTCACGGGGTCCAAGGGCAAGAGCACCACGGCAAGCCTCCTCCACCACCTCCTAAAGCGGGCCCAGGGCGGAAGCG
CCCTCCTCTCCACCGTGGGCCTCCGCCTGGGCGAGGAGGCCCGCCCCCCCGTGGGCCACTTCACCACCCCGGAAGCCCCC
GAGGTCTACGCCTTCCTCCGGGAGGCGGCGGACCGCGGCCTGAAAAGCGCCGTGCTGGAGGTCTCCAGCCACGCCCTGGC
CCTGAAGCGGGTGGAGGGCCTCACCTTCCGGGTGGGGGTCTTCGTGAACTTCTACCCCGACGACCACCTGGACTTCCACG
GCACCGCCGAGGCCTACTTCCGGGCCAAGGCCCTCCTGGTGGAGCGGTCGGACCTCGCCGTCCTCGCCCGTAGCCTCCCC
CACTTCCCCCGGCTCGCCCAGAGGCCCCACCTGAGCTTCGGGGAGGGAGGGGAGGTCTATGCGGAGGGGCTTCGGGAAAC
CCCGGAGGGCCTGGCCTTCCGCCTCCACACCCCCTGGGGGTCAGGGGAGGCCTTCTTGCCCCTCCTCGGGGCCTACAACC
TGGAAAACGCCCTCGCCGCAAGCGCCGCCGCCCTGGCCCTGGGGGTGGGCCTCGAGGCGGTGCTGGAGGGCCTCGCCACC
TTCCCCGGGGTGCCGGGCCGGATGGAGGTGGTCCTCAAGGCCCCCTTCCGCGTGGTCATTGACTTCGCCCACACCGGGAA
GAGCCTGGAGGCCGCCCTGAAGACCCTCCGCCCCACCACCCCGGGGAGGCTCCTTTTGGTGGTGGGGGCGGCCGGGGAGC
GGGACCCCCAGCGGCGCGTGGACATCGGCCGGGTGGCGGCCCGCCTCGCGGACAAGACCTTCTTCACCGAGGAGGACCAC
CGGGGCGAGGACCTCTTCGCCATCCTGGAGGCCATGGCCCAGGCGGCCCGGGCCGAGGGCGGGGTCTACGAGCTCGTCCC
CGACCGGGAGGAGGCCATCCTCAAGGCCATCCTCGAGGCCCGCCCCGGGGACACGGTCCTGCTCGCCGGCAAGGGGCACG
AGGCCACCTTGGAGCGGGGCCAGGTGGCCCTGCCCTGGAACGAGCGGGAGGCCGCCCTCAAAGCCCTCCGGGCGCGGGGG
CTTCCCTGA

Upstream 100 bases:

>100_bases
CAGCGCCGCCCCGGGGACCGGGTCCGCCTCACCCTCTGGCGGGACCGGAGGCGGCTGGAGGTCACCGTGGTCCTGATGGC
CCGGCCGCGGGAGTGAGGCC

Downstream 100 bases:

>100_bases
CCAGCATCCCCTCGAGGAGGAGCCTGAGGTTCTTCTCCAGCTCCTCGGCCAGGTCCCGCCCCGGGGTGTAGGCGGCCCAG
CGCAGGGCGGAGAGGAAGTA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MPTLKELFALFGKEAPEVPVRGLTLDSRRVEPGFVFVAVPGVPLPHRKPLDGHDFVPEALARGAVAVVGERPLALPVPYL
QVADGRQALALLARRFHGEPDRKLLLVGVTGSKGKSTTASLLHHLLKRAQGGSALLSTVGLRLGEEARPPVGHFTTPEAP
EVYAFLREAADRGLKSAVLEVSSHALALKRVEGLTFRVGVFVNFYPDDHLDFHGTAEAYFRAKALLVERSDLAVLARSLP
HFPRLAQRPHLSFGEGGEVYAEGLRETPEGLAFRLHTPWGSGEAFLPLLGAYNLENALAASAAALALGVGLEAVLEGLAT
FPGVPGRMEVVLKAPFRVVIDFAHTGKSLEAALKTLRPTTPGRLLLVVGAAGERDPQRRVDIGRVAARLADKTFFTEEDH
RGEDLFAILEAMAQAARAEGGVYELVPDREEAILKAILEARPGDTVLLAGKGHEATLERGQVALPWNEREAALKALRARG
LP

Sequences:

>Translated_482_residues
MPTLKELFALFGKEAPEVPVRGLTLDSRRVEPGFVFVAVPGVPLPHRKPLDGHDFVPEALARGAVAVVGERPLALPVPYL
QVADGRQALALLARRFHGEPDRKLLLVGVTGSKGKSTTASLLHHLLKRAQGGSALLSTVGLRLGEEARPPVGHFTTPEAP
EVYAFLREAADRGLKSAVLEVSSHALALKRVEGLTFRVGVFVNFYPDDHLDFHGTAEAYFRAKALLVERSDLAVLARSLP
HFPRLAQRPHLSFGEGGEVYAEGLRETPEGLAFRLHTPWGSGEAFLPLLGAYNLENALAASAAALALGVGLEAVLEGLAT
FPGVPGRMEVVLKAPFRVVIDFAHTGKSLEAALKTLRPTTPGRLLLVVGAAGERDPQRRVDIGRVAARLADKTFFTEEDH
RGEDLFAILEAMAQAARAEGGVYELVPDREEAILKAILEARPGDTVLLAGKGHEATLERGQVALPWNEREAALKALRARG
LP
>Mature_481_residues
PTLKELFALFGKEAPEVPVRGLTLDSRRVEPGFVFVAVPGVPLPHRKPLDGHDFVPEALARGAVAVVGERPLALPVPYLQ
VADGRQALALLARRFHGEPDRKLLLVGVTGSKGKSTTASLLHHLLKRAQGGSALLSTVGLRLGEEARPPVGHFTTPEAPE
VYAFLREAADRGLKSAVLEVSSHALALKRVEGLTFRVGVFVNFYPDDHLDFHGTAEAYFRAKALLVERSDLAVLARSLPH
FPRLAQRPHLSFGEGGEVYAEGLRETPEGLAFRLHTPWGSGEAFLPLLGAYNLENALAASAAALALGVGLEAVLEGLATF
PGVPGRMEVVLKAPFRVVIDFAHTGKSLEAALKTLRPTTPGRLLLVVGAAGERDPQRRVDIGRVAARLADKTFFTEEDHR
GEDLFAILEAMAQAARAEGGVYELVPDREEAILKAILEARPGDTVLLAGKGHEATLERGQVALPWNEREAALKALRARGL
P

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=493, Percent_Identity=35.2941176470588, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1786274, Length=387, Percent_Identity=28.6821705426357, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51793; Mature: 51662

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLKELFALFGKEAPEVPVRGLTLDSRRVEPGFVFVAVPGVPLPHRKPLDGHDFVPEAL
CCCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
ARGAVAVVGERPLALPVPYLQVADGRQALALLARRFHGEPDRKLLLVGVTGSKGKSTTAS
HCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHH
LLHHLLKRAQGGSALLSTVGLRLGEEARPPVGHFTTPEAPEVYAFLREAADRGLKSAVLE
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
VSSHALALKRVEGLTFRVGVFVNFYPDDHLDFHGTAEAYFRAKALLVERSDLAVLARSLP
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHEEECHHHHHHHHHCC
HFPRLAQRPHLSFGEGGEVYAEGLRETPEGLAFRLHTPWGSGEAFLPLLGAYNLENALAA
CCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCCHHHHHHH
SAAALALGVGLEAVLEGLATFPGVPGRMEVVLKAPFRVVIDFAHTGKSLEAALKTLRPTT
HHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEHHHCCHHHHHHHHHHCCCC
PGRLLLVVGAAGERDPQRRVDIGRVAARLADKTFFTEEDHRGEDLFAILEAMAQAARAEG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
GVYELVPDREEAILKAILEARPGDTVLLAGKGHEATLERGQVALPWNEREAALKALRARG
CEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCEECCCCHHHHHHHHHHHCC
LP
CC
>Mature Secondary Structure 
PTLKELFALFGKEAPEVPVRGLTLDSRRVEPGFVFVAVPGVPLPHRKPLDGHDFVPEAL
CCHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
ARGAVAVVGERPLALPVPYLQVADGRQALALLARRFHGEPDRKLLLVGVTGSKGKSTTAS
HCCCEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHH
LLHHLLKRAQGGSALLSTVGLRLGEEARPPVGHFTTPEAPEVYAFLREAADRGLKSAVLE
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
VSSHALALKRVEGLTFRVGVFVNFYPDDHLDFHGTAEAYFRAKALLVERSDLAVLARSLP
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHEEECHHHHHHHHHCC
HFPRLAQRPHLSFGEGGEVYAEGLRETPEGLAFRLHTPWGSGEAFLPLLGAYNLENALAA
CCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCCHHHHHHH
SAAALALGVGLEAVLEGLATFPGVPGRMEVVLKAPFRVVIDFAHTGKSLEAALKTLRPTT
HHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCEEEEEEHHHCCHHHHHHHHHHCCCC
PGRLLLVVGAAGERDPQRRVDIGRVAARLADKTFFTEEDHRGEDLFAILEAMAQAARAEG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
GVYELVPDREEAILKAILEARPGDTVLLAGKGHEATLERGQVALPWNEREAALKALRARG
CEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCEECCCCHHHHHHHHHHHCC
LP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 8289242 [H]