Click here to switch to the map view.

The map label for this gene is trmL [H]

Identifier: 55980023

GI number: 55980023

Start: 52473

End: 52922

Strand: Reverse

Name: trmL [H]

Synonym: TTHA0054

Alternate gene names: 55980023

Gene position: 52922-52473 (Counterclockwise)

Preceding gene: 55980024

Following gene: 55980021

Centisome position: 2.86

GC content: 70.89

Gene sequence:

>450_bases
ATGCTCCATCTGGTCCTCTACCAGCCGGAGATCCCCCAAAACGCCGGGAACGTGGCCCGCACCGCGGCCGCCCTGGGATG
GCCCCTCCACCTCATCCGTCCCCTGGGCTTCCTCCTCTCCAGCCCCAAGCTCAAGCGGGCGGGCCTGGACTACTGGCCCC
ACGTGGACCTCAGGCTCCACGACTCCTTTGCGGCGTTCCTGGAGGCCCTGCCCCGGGGGGCGCGGGTCTTCGCCTTCAGC
GCCAGGGGGGAAGCCTCCCTCTACGAGGCCCGCTTCCGGGAGGGGGACTACCTCCTCTTCGGCCCCGAAAGCCGGGGGCT
TCCCGAGGAGGTCCTCGCCCGCTTCCCCACCCTAAAGATCCCCATGCCGGGGCCGGTGCGCTCCTTGAACCTGGCGGTGG
CCGTGGGGGTGGCGGCCTACGAGGCCTACCGCCAGCTCACAGGGCGATGA

Upstream 100 bases:

>100_bases
TTTCCCGCAAAAGGGCCCTGGAGCTTCTCCGCCGCCTCATCCGGGAGGGCCGGGTGGAGAGGGTGGGCCGGGGGGCGGCC
ACCCGCTACCGGAGGCGGTG

Downstream 100 bases:

>100_bases
GGGCGTAGCCCTGGGCGTGGACCACCTCCACGCCCAAAGGGGTGCGGTGCCGCCTTTCGGGGTTCGCCCCGAGGAGGGGG
GTGTGGCCGTGGACGTGGAG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MLHLVLYQPEIPQNAGNVARTAAALGWPLHLIRPLGFLLSSPKLKRAGLDYWPHVDLRLHDSFAAFLEALPRGARVFAFS
ARGEASLYEARFREGDYLLFGPESRGLPEEVLARFPTLKIPMPGPVRSLNLAVAVGVAAYEAYRQLTGR

Sequences:

>Translated_149_residues
MLHLVLYQPEIPQNAGNVARTAAALGWPLHLIRPLGFLLSSPKLKRAGLDYWPHVDLRLHDSFAAFLEALPRGARVFAFS
ARGEASLYEARFREGDYLLFGPESRGLPEEVLARFPTLKIPMPGPVRSLNLAVAVGVAAYEAYRQLTGR
>Mature_149_residues
MLHLVLYQPEIPQNAGNVARTAAALGWPLHLIRPLGFLLSSPKLKRAGLDYWPHVDLRLHDSFAAFLEALPRGARVFAFS
ARGEASLYEARFREGDYLLFGPESRGLPEEVLARFPTLKIPMPGPVRSLNLAVAVGVAAYEAYRQLTGR

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=45.6375838926174, Blast_Score=132, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16507; Mature: 16507

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLVLYQPEIPQNAGNVARTAAALGWPLHLIRPLGFLLSSPKLKRAGLDYWPHVDLRLH
CEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEC
DSFAAFLEALPRGARVFAFSARGEASLYEARFREGDYLLFGPESRGLPEEVLARFPTLKI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCEEEC
PMPGPVRSLNLAVAVGVAAYEAYRQLTGR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHLVLYQPEIPQNAGNVARTAAALGWPLHLIRPLGFLLSSPKLKRAGLDYWPHVDLRLH
CEEEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEC
DSFAAFLEALPRGARVFAFSARGEASLYEARFREGDYLLFGPESRGLPEEVLARFPTLKI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCEEEC
PMPGPVRSLNLAVAVGVAAYEAYRQLTGR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]