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The map label for this gene is dus2 [H]

Identifier: 55979985

GI number: 55979985

Start: 17121

End: 18149

Strand: Direct

Name: dus2 [H]

Synonym: TTHA0016

Alternate gene names: 55979985

Gene position: 17121-18149 (Clockwise)

Preceding gene: 55979984

Following gene: 55979992

Centisome position: 0.93

GC content: 68.51

Gene sequence:

>1029_bases
GTGCTTGACCCGAGGCTTTCCGTGGCCCCCATGGTGGACCGCACCGACCGGCACTTCCGCTTTCTGGTGCGGCAGGTGAG
CCTGGGGGTGAGGCTCTACACGGAGATGACCGTGGACCAGGCGGTGCTGAGGGGAAACCGGGAGCGCCTCCTCGCCTTCC
GGCCCGAGGAGCACCCCATCGCCCTCCAGCTTGCGGGTTCCGACCCAAAGAGCCTGGCGGAGGCGGCGAGGATCGGCGAG
GCCTTCGGCTATGACGAGATCAACCTCAACCTGGGCTGCCCCTCGGAGAAGGCCCAGGAAGGGGGATACGGGGCCTGCCT
CCTCTTGGACCTCGCGCGGGTGCGAGAGATCCTGAAGGCCATGGGGGAGGCCGTCCGCGTCCCCGTGACGGTGAAGATGC
GCCTGGGCCTCGAGGGGAAGGAGACCTACCGGGGGCTTGCCCAAAGCGTGGAGGCCATGGCGGAGGCGGGGGTCAAGGTC
TTCGTGGTCCACGCGAGGAGCGCCCTCCTCGCCCTCTCCACAAAGGCGAACCGCGAGATCCCCCCCTTGCGCCACGACTG
GGTCCACCGGCTCAAAGGGGACTTTCCCCAGCTCACCTTCGTCACCAACGGGGGGATAAGGAGCCTGGAGGAGGCCCTCT
TCCACCTGAAGCGGGTGGACGGGGTGATGCTGGGGCGGGCGGTCTACGAGGACCCCTTCGTCCTGGAGGAGGCGGACCGG
AGGGTCTTTGGCCTTCCCCGCCGTCCAAGCCGCCTCGAGGTGGCCCGGAGGATGCGGGCCTACCTGGAGGAGGAGGTCCT
AAAGGGCACGCCCCCTTGGGCGGTCCTGAGGCACATGCTCAACCTCTTCCGGGGAAGGCCCAAGGGGAGGCTCTGGCGGA
GGCTTTTGAGCGAGGGCCGCTCCCTTCAGGCCCTGGACCGGGCCCTACGGCTTATGGAGGAAGAGGTAGGCGAGGAGGGC
GAAAAGGAGAAGCCAGGCCCAAGGGGGCAGAGGGAAGCGGCGCCTGGGCCTGCCCGTGAGGGGGTCTAA

Upstream 100 bases:

>100_bases
AAGGCCCAAAACCCGGGGCTGGAGGTGGTGGAAGAAGGGGCGTGGGAGACGATCGCCTTCCGCGAGCCCAAGCCCCTCTC
CCCCGAGGAGGTCCTGAAGG

Downstream 100 bases:

>100_bases
GGAGGGGGGTGGGGCCATCTTCAGGCGGGTCGCCCGCTTCATGTGGGCCACCATCTTGTCCAGGTCCCCCTTCCGCTTGT
AGAGGGCGGCCAGGTTCTCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLLAFRPEEHPIALQLAGSDPKSLAEAARIGE
AFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKV
FVVHARSALLALSTKANREIPPLRHDWVHRLKGDFPQLTFVTNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADR
RVFGLPRRPSRLEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGRSLQALDRALRLMEEEVGEEG
EKEKPGPRGQREAAPGPAREGV

Sequences:

>Translated_342_residues
MLDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLLAFRPEEHPIALQLAGSDPKSLAEAARIGE
AFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKV
FVVHARSALLALSTKANREIPPLRHDWVHRLKGDFPQLTFVTNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADR
RVFGLPRRPSRLEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGRSLQALDRALRLMEEEVGEEG
EKEKPGPRGQREAAPGPAREGV
>Mature_342_residues
MLDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLLAFRPEEHPIALQLAGSDPKSLAEAARIGE
AFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKV
FVVHARSALLALSTKANREIPPLRHDWVHRLKGDFPQLTFVTNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADR
RVFGLPRRPSRLEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGRSLQALDRALRLMEEEVGEEG
EKEKPGPRGQREAAPGPAREGV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=232, Percent_Identity=31.4655172413793, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI40807366, Length=240, Percent_Identity=30.4166666666667, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=46.7084639498433, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1789660, Length=236, Percent_Identity=29.6610169491525, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=30.0411522633745, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=260, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=240, Percent_Identity=25, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=275, Percent_Identity=26.5454545454545, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580595, Length=287, Percent_Identity=26.4808362369338, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920448, Length=287, Percent_Identity=26.4808362369338, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38474; Mature: 38474

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLLAFRPEEHPI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCE
ALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKA
EEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
MGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKVFVVHARSALLALSTKANREI
HHHHEECCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCCC
PPLRHDWVHRLKGDFPQLTFVTNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADR
CCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
RVFGLPRRPSRLEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGR
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
SLQALDRALRLMEEEVGEEGEKEKPGPRGQREAAPGPAREGV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLLAFRPEEHPI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCE
ALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQEGGYGACLLLDLARVREILKA
EEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHH
MGEAVRVPVTVKMRLGLEGKETYRGLAQSVEAMAEAGVKVFVVHARSALLALSTKANREI
HHHHEECCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCCCCCC
PPLRHDWVHRLKGDFPQLTFVTNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADR
CCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCC
RVFGLPRRPSRLEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGR
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
SLQALDRALRLMEEEVGEEGEKEKPGPRGQREAAPGPAREGV
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]