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The map label for this gene is rnhB

Identifier: 54297282

GI number: 54297282

Start: 1481387

End: 1481962

Strand: Direct

Name: rnhB

Synonym: lpp1327

Alternate gene names: 54297282

Gene position: 1481387-1481962 (Clockwise)

Preceding gene: 54297278

Following gene: 54297285

Centisome position: 42.28

GC content: 40.1

Gene sequence:

>576_bases
ATGAATACTGAGCTAAAAATATTGATGGCTGGGGTCGATGAAGTGGGGAGAGGACCACTTGCAGGTGCCGTTGTGACCGC
AGCCGTTATTTTAAAAAAACCAATAGATGGGCTTACCGACTCAAAAAAATTAAGTCCAAAACAAAGAAATTTATTAGCAA
TTAGAATAAAGGAAGAAGCGTTAGCTTTTGCCTATGGGCGAGCAGAAGTTGAGGAAATAGATCAATTAAATATTCATCAT
GCTACTCTATTAGCTATGCGAAGGGCAGTTGAAGCTTTACCCATTCAGCCAGATAATGTCGTTGTCGATGGAGCTTTTAC
TCCTCAATTGAACATACCATGCAAGGCTATTGTCCAGGGAGATAGCTTGATACCTGAAATTAGTGCGGCATCCATCCTCG
CCAAGGTATTGCGTGATGAAGAAATGGTAGCTCTTGATAAAATATATCCAGGATATGGTTTTGCAGAACATAAAGGTTAT
GCAACCCCTGTCCATAAAGAAGCGCTAATGCGTTTGGGTCCTTGTAAAATACATAGAAGAAGTTACTCCCCTGTTGCAGA
TTTGATTTCAAAATAA

Upstream 100 bases:

>100_bases
AACTCCAGAGATTGGTCCAAATCAAAGAAGTCGATTCTTGTCTTGCACTTGCATTCAATAGCTATTATCTTACGCCTAAT
TTTTCGCAGGGAGCAGTTAA

Downstream 100 bases:

>100_bases
AACTCTGAGTGGTATTAATTTGGAAAAATACCGCCCAAATAAAAATACATAAGAAATAGCTTTTAAAATGTCATAATATG
ATTTATGCAAATCAGAGCAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHH
ATLLAMRRAVEALPIQPDNVVVDGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
ATPVHKEALMRLGPCKIHRRSYSPVADLISK

Sequences:

>Translated_191_residues
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHH
ATLLAMRRAVEALPIQPDNVVVDGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
ATPVHKEALMRLGPCKIHRRSYSPVADLISK
>Mature_191_residues
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEALAFAYGRAEVEEIDQLNIHH
ATLLAMRRAVEALPIQPDNVVVDGAFTPQLNIPCKAIVQGDSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGY
ATPVHKEALMRLGPCKIHRRSYSPVADLISK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=62.2222222222222, Blast_Score=211, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_LEGPA (Q5X5J3)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123651.1
- HSSP:   Q9X017
- ProteinModelPortal:   Q5X5J3
- STRING:   Q5X5J3
- GeneID:   3117179
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1327
- LegioList:   lpp1327
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   LPNE297246:LPP1327-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 20754; Mature: 20754

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEA
CCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHH
LAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVVVDGAFTPQLNIPCKAIVQG
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEECC
DSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGYATPVHKEALMRLGPCKIHRR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHEECC
SYSPVADLISK
CCCHHHHHHCC
>Mature Secondary Structure
MNTELKILMAGVDEVGRGPLAGAVVTAAVILKKPIDGLTDSKKLSPKQRNLLAIRIKEEA
CCCCEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHHH
LAFAYGRAEVEEIDQLNIHHATLLAMRRAVEALPIQPDNVVVDGAFTPQLNIPCKAIVQG
HHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEECC
DSLIPEISAASILAKVLRDEEMVALDKIYPGYGFAEHKGYATPVHKEALMRLGPCKIHRR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHEECC
SYSPVADLISK
CCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA