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The map label for this gene is trpA

Identifier: 54297224

GI number: 54297224

Start: 1406452

End: 1407270

Strand: Direct

Name: trpA

Synonym: lpp1269

Alternate gene names: 54297224

Gene position: 1406452-1407270 (Clockwise)

Preceding gene: 54297223

Following gene: 54297225

Centisome position: 40.14

GC content: 40.42

Gene sequence:

>819_bases
ATGAACCGTATTGATAAGACTCTGGAAAAATTAAAAGCCAATAGAAAAAAAATGTTAAGTCCTTATATTACTGCAGGCGA
TCCATATCCTGAGTTAACAGTAAGTTTAATGCATCAATTGGTTAAATCAGGGGCAGATGTATTGGAGCTGGGAATACCTT
TTTCAGACCCTATGGCAGAAGGTCCTGTTATCCAAAGAGCAATGGAACGAGCTTTGGCGCATTCAATTCATTGTGATGAC
GTATTGAATATGGTTAGGCAATTTCGCAAAACGGATACCGAAACGCCTGTGATTCTTATGGGATATTTAAATCCTATAGA
ACAATATGGCTATGATCTTTTTGCTCAACAGGCAGTGGAGGCTGGGGCTGATGGAACAATATTAGTTGATTTGCCTCCTG
AGGAAGCTGATGGAGTATCGCGAGTTTGGCAAAAACATGGTTTGTACAGTATTTATTTATGTTCGCCAACCACCTCAGCT
GAAAGAATGAACTTTATAAATCAGCATGCCAATGGTTATCTGTATTATGTTTCATTGAAGGGAGTGACTGGTTCTGACGC
GCTTAAGTTGCCAGAATTAAAAGCTCAATATCTACAACGAAAGGCACAATCAAAATTACCGCTTATGGTTGGGTTTGGAA
TAAAAACTCCGGAAATGGCTGCACAAGTTGCTGAATTTGCTGATGGGGTCATTGTTGGGGCGGCACTGATAAATGAAATC
ATTGAAGCCTATGAGGCCAAGAAAGATCCGCTACAAGCAAGCGGGGCTCTATTGAGTTCCATGAGGCAAGCTATCGATAA
TATTGGAAGTATGGTATGA

Upstream 100 bases:

>100_bases
ATCATAGTCAATCTATCTGGTCGTGGTGATAAAGATATGCATACAGTAGCTGCTATTGACGGTATAACAATTTAATTTTG
ATAAATTATTCAGGTACAAC

Downstream 100 bases:

>100_bases
CAGAACAAACTGAAAAAAGAGCTCATTTTATTAGACAACTCATTATAGATGATTTGGCAAATGGAAAACATCAGGGAATC
GTTACTCGTTTTCCTCCTGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDD
VLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVEAGADGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV

Sequences:

>Translated_272_residues
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDD
VLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVEAGADGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV
>Mature_272_residues
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAEGPVIQRAMERALAHSIHCDD
VLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVEAGADGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSA
ERMNFINQHANGYLYYVSLKGVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=250, Percent_Identity=32.4, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=249, Percent_Identity=39.3574297188755, Blast_Score=172, Evalue=7e-44,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_LEGPA (Q5X5Q1)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123593.1
- ProteinModelPortal:   Q5X5Q1
- SMR:   Q5X5Q1
- STRING:   Q5X5Q1
- GeneID:   3118995
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1269
- LegioList:   lpp1269
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   LPNE297246:LPP1269-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29893; Mature: 29893

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA; PS00201 FLAVODOXIN

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHHHHHHHH
AGADGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSAERMNFINQHANGYLYYVSLK
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHH
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRIDKTLEKLKANRKKMLSPYITAGDPYPELTVSLMHQLVKSGADVLELGIPFSDPMAE
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRAMERALAHSIHCDDVLNMVRQFRKTDTETPVILMGYLNPIEQYGYDLFAQQAVE
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEHHHCHHHHHHHHHHHHHHHH
AGADGTILVDLPPEEADGVSRVWQKHGLYSIYLCSPTTSAERMNFINQHANGYLYYVSLK
CCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEEEC
GVTGSDALKLPELKAQYLQRKAQSKLPLMVGFGIKTPEMAAQVAEFADGVIVGAALINEI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHH
IEAYEAKKDPLQASGALLSSMRQAIDNIGSMV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA