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The map label for this gene is trpB

Identifier: 54297223

GI number: 54297223

Start: 1405227

End: 1406426

Strand: Direct

Name: trpB

Synonym: lpp1268

Alternate gene names: 54297223

Gene position: 1405227-1406426 (Clockwise)

Preceding gene: 54297222

Following gene: 54297224

Centisome position: 40.11

GC content: 41.83

Gene sequence:

>1200_bases
ATGATTAAAAAAGAGCTTCCAGATGAGTTTGGCCATTTTGGCCCATATGGCGGTATGTTTGTAGCCGATACATTAGTTCA
TGCTTTAAAACAATTAGAGCATGCCTATACCAAATATCGTAATGATCAGGATTTTCTGTCTGAGTTACACACAGAATTAA
AAGATTATGTGGGGCGGCCCAATCCTCTATACCATGCCGTACACTTAAGTAAAAAGATAGGCGGTGCACAAATTTATCTC
AAGCGTGAAGATTTAAATCATACCGGCGCTCATAAAATCAATAATACCATTGGACAAGCCTTACTCGCCAAACGCATGGG
GAAAACTCGAGTGATCGCAGAAACTGGCGCAGGTCAACATGGCGTTGCAACTGCTACAGTTGCCGCTAAATTTGGGTTTC
AATGCGTTGTGTACATGGGGTCAGAAGACATTAAGCGTCAATCCAGTAATGTTTATAGAATGAAATTGTTAGGTGCTGAA
GTGGTGCCTGTCACTTCAGGATCAAAGACGTTGAAAGATGCTTTAAATGAAGCATTGAGAGATTGGGTGAGTCATGTCGA
CGACACGTTTTATATTATTGGTACTGTTGCAGGTCCCCATCCTTATCCTCAAATGGTAAGAGATTTTCAAGCGATTATTG
GTGTTGAAGCTCGAGCTCAGCATATGGAAAAAACAGGCCGTTTGCCAGATGCTTTGGTAGCTTGTGTCGGAGGTGGATCC
AATGCAATAGGCTTGTTTTATCCCTTTTTGAATGATCAGTCTGTTATGATTTATGGGGTTGAAGCAGGAGGGAAAGGCAT
AGAAACCGGTGAGCATTCAGCATCTCTGATTGCTGGAAAACCTGGTGTGTTACACGGTAATAGAACCTATTTATTGTGTG
ATGAGTATGGACAGGTGAAAGACACTCATTCAGTATCAGCCGGTCTGGATTATCCTGGAGTTGGGCCTGAACATGCTTAT
TTAAAAGATACTGGACGGGTCATTTATAAAGCAATAAATGATTCCGAGGCCCTGGATGCCTTTCGCCTGTTAACTCATAC
GGAAGGAATTATTCCAGCATTAGAATCTAGCCACGCGGTAGCCTATGCCATACAATTGGCTAAAACGATGTCAAAAGAAC
AGAGCATCATAGTCAATCTATCTGGTCGTGGTGATAAAGATATGCATACAGTAGCTGCTATTGACGGTATAACAATTTAA

Upstream 100 bases:

>100_bases
CATCCTTATGCAGTGGATGTGTGCAGTGGTATAGAAGCTTCACCCGGAGTAAAAGATCATTTGAAAATGAGCCGATTTAT
AAAGGCAATATGGGGATAGT

Downstream 100 bases:

>100_bases
TTTTGATAAATTATTCAGGTACAACATGAACCGTATTGATAAGACTCTGGAAAAATTAAAAGCCAATAGAAAAAAAATGT
TAAGTCCTTATATTACTGCA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQDFLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYL
KREDLNHTGAHKINNTIGQALLAKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEARAQHMEKTGRLPDALVACVGGGS
NAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASLIAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAY
LKDTGRVIYKAINDSEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVAAIDGITI

Sequences:

>Translated_399_residues
MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQDFLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYL
KREDLNHTGAHKINNTIGQALLAKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEARAQHMEKTGRLPDALVACVGGGS
NAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASLIAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAY
LKDTGRVIYKAINDSEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVAAIDGITI
>Mature_399_residues
MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQDFLSELHTELKDYVGRPNPLYHAVHLSKKIGGAQIYL
KREDLNHTGAHKINNTIGQALLAKRMGKTRVIAETGAGQHGVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAE
VVPVTSGSKTLKDALNEALRDWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEARAQHMEKTGRLPDALVACVGGGS
NAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASLIAGKPGVLHGNRTYLLCDEYGQVKDTHSVSAGLDYPGVGPEHAY
LKDTGRVIYKAINDSEALDAFRLLTHTEGIIPALESSHAVAYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVAAIDGITI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=55.6430446194226, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321412, Length=401, Percent_Identity=56.1097256857855, Blast_Score=467, Evalue=1e-132,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_LEGPA (Q5X5Q2)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123592.1
- HSSP:   Q8U093
- ProteinModelPortal:   Q5X5Q2
- SMR:   Q5X5Q2
- STRING:   Q5X5Q2
- GeneID:   3117089
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1268
- LegioList:   lpp1268
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   LPNE297246:LPP1268-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43302; Mature: 43302

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQDFLSELHTELKDYVGRP
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
NPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALLAKRMGKTRVIAETGAGQH
CCHHHHEEHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAEVVPVTSGSKTLKDALNEALR
CCHHHHHHHHCCCEEEEEECCHHHHHHCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHH
DWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEARAQHMEKTGRLPDALVACVGGGS
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCC
NAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASLIAGKPGVLHGNRTYLLCDEYGQVK
CEEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEECCCCEEECCEEEEEECCCCCCC
DTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAINDSEALDAFRLLTHTEGIIPALESSHAV
CCCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHH
AYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVAAIDGITI
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MIKKELPDEFGHFGPYGGMFVADTLVHALKQLEHAYTKYRNDQDFLSELHTELKDYVGRP
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
NPLYHAVHLSKKIGGAQIYLKREDLNHTGAHKINNTIGQALLAKRMGKTRVIAETGAGQH
CCHHHHEEHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GVATATVAAKFGFQCVVYMGSEDIKRQSSNVYRMKLLGAEVVPVTSGSKTLKDALNEALR
CCHHHHHHHHCCCEEEEEECCHHHHHHCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHH
DWVSHVDDTFYIIGTVAGPHPYPQMVRDFQAIIGVEARAQHMEKTGRLPDALVACVGGGS
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCC
NAIGLFYPFLNDQSVMIYGVEAGGKGIETGEHSASLIAGKPGVLHGNRTYLLCDEYGQVK
CEEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCEEEECCCCEEECCEEEEEECCCCCCC
DTHSVSAGLDYPGVGPEHAYLKDTGRVIYKAINDSEALDAFRLLTHTEGIIPALESSHAV
CCCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCHH
AYAIQLAKTMSKEQSIIVNLSGRGDKDMHTVAAIDGITI
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA