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The map label for this gene is truA

Identifier: 54297221

GI number: 54297221

Start: 1403813

End: 1404601

Strand: Direct

Name: truA

Synonym: lpp1266

Alternate gene names: 54297221

Gene position: 1403813-1404601 (Clockwise)

Preceding gene: 54297218

Following gene: 54297222

Centisome position: 40.07

GC content: 41.32

Gene sequence:

>789_bases
ATGCGTATTGCCTTAGTGGTTGAATACGATGGTAGCCAATATCACGGCTGGCAGGCTCAAACGGGTTTGCATACTATTCA
ACAAGCTGTTGAATTTGCATTATCAAAAGTTGCAGACAGTTCTATTTCTGTTGTCTGCGCAGGTAGAACAGATACTGGGG
TCCATGCGACCAATCAGGTAATTCATTTTGATTGTGAGAAAGATCGCAGCATTCGAGCCTGGATACATGGTGCTAATACC
TTTTTACCTAAAGACATCTGCGTCAAATGGGGCAAGGAAATGCCAGAAAATTTTCATGCTCGTTATTCTGCTGTGAGTCG
GCGGTATCGCTATGTCATTTATAATGGAGCTATACGGCCAGGGTTATTACGCGGTAATGTGACATGGCAATATCGGCAGT
TGGATCATCGTTTGATGCAACAGGGTGGACAATGTCTTCTGGGCGAAAATGATTTTACTTCATTCCGTTCCGTCGAATGT
CAATCTAATACACCAATGCGTAATATTCATCAATTGCAAGTTACAAGACATGGTGATTTGGTTGTTCTTGATATTACAGC
GAATGCCTTTTTACATCATATGGTAAGAAATATAGCTGGTGTATTGATTGCTGTTGGCTCGGGAAAACACCCTGTTGGTT
GGGTTAAAGATGTTTTAAATGCAAAGGATAGAAAATTAGGCGCTGAAACTGCACCGTCATATGGCCTATATTTGGTCCAG
GTGACTTATCCCAAAGAATTTGGATTGTTGCAAAATAATCCAGGCCCTTTGTTTCTTTGGGAGAAATAA

Upstream 100 bases:

>100_bases
CAAAATGATAAAACCCATACAGGCAAAGAGTTAATACTCTAAAAATTATAGACAAAACAAAGAGGGTGTTATAAATTGAC
CCTTTTATCAGGAAAATTGA

Downstream 100 bases:

>100_bases
TTGAATCCATCTCGTATAAGAATCAAAATGTGTGGAATGACACGTTCAGAAGATATTCAATACGCCATTGATTTAGGGGT
GGACGCGATTGGCTTAATAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANT
FLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
VTYPKEFGLLQNNPGPLFLWEK

Sequences:

>Translated_262_residues
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANT
FLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
VTYPKEFGLLQNNPGPLFLWEK
>Mature_262_residues
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQVIHFDCEKDRSIRAWIHGANT
FLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRPGLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVEC
QSNTPMRNIHQLQVTRHGDLVVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
VTYPKEFGLLQNNPGPLFLWEK

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=274, Percent_Identity=29.9270072992701, Blast_Score=94, Evalue=9e-20,
Organism=Escherichia coli, GI1788657, Length=258, Percent_Identity=55.0387596899225, Blast_Score=301, Evalue=3e-83,
Organism=Drosophila melanogaster, GI19922728, Length=265, Percent_Identity=27.5471698113208, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_LEGPA (Q5X5Q4)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123590.1
- ProteinModelPortal:   Q5X5Q4
- SMR:   Q5X5Q4
- STRING:   Q5X5Q4
- GeneID:   3118886
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1266
- LegioList:   lpp1266
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   LPNE297246:LPP1266-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29559; Mature: 29559

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQV
CEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEE
IHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRP
EEEECCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEECCCCC
GLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVECQSNTPMRNIHQLQVTRHGDL
CEEECCCEEEHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCEEEEEEEECCCE
VVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
EEEEECHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCHHCCCCCCCCCCCEEEEE
VTYPKEFGLLQNNPGPLFLWEK
EECCCCCCCEECCCCCEEEECC
>Mature Secondary Structure
MRIALVVEYDGSQYHGWQAQTGLHTIQQAVEFALSKVADSSISVVCAGRTDTGVHATNQV
CEEEEEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEE
IHFDCEKDRSIRAWIHGANTFLPKDICVKWGKEMPENFHARYSAVSRRYRYVIYNGAIRP
EEEECCCCCCEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEEECCCCC
GLLRGNVTWQYRQLDHRLMQQGGQCLLGENDFTSFRSVECQSNTPMRNIHQLQVTRHGDL
CEEECCCEEEHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCCEEEEEEEECCCE
VVLDITANAFLHHMVRNIAGVLIAVGSGKHPVGWVKDVLNAKDRKLGAETAPSYGLYLVQ
EEEEECHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCHHCCCCCCCCCCCEEEEE
VTYPKEFGLLQNNPGPLFLWEK
EECCCCCCCEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA