Click here to switch to the map view.

The map label for this gene is pdxJ

Identifier: 54296967

GI number: 54296967

Start: 1119775

End: 1120521

Strand: Direct

Name: pdxJ

Synonym: lpp1008

Alternate gene names: 54296967

Gene position: 1119775-1120521 (Clockwise)

Preceding gene: 54296964

Following gene: 54296968

Centisome position: 31.96

GC content: 42.57

Gene sequence:

>747_bases
ATGAATAAAGAGTTATTGTTAGGTGTTAATATTGATCATATAGCAACCATAAGACAAGCACGAGGAACTCGTTATCCTGA
TCCTGTTCAGGCAGCAATGGATGCTGAAGAAGCTGGGGCAGATGGAATTACTTTGCATATGAGAGAGGACTTACGACATA
TTCAGGCGCGTGATGTCAGATTGATTAAACAGGTTCTGCAAACACGTATGAATCTTGAGCTGGCGGTTACTGAAGCCATG
CTTGATTTTGCCGAGGAAATATTGCCGGAGCATGCTTGCTTAGTGCCTGAAAAGCGGGAAGAACTAACTACAGAAGGTGG
ATTAGATATTTTGACTCACCGCAATGTGGTGGAAAGGGCAGTACGTCGTTTACAATTAATGGGAAGTGAGGTTTCTTTAT
TTATTGACCCGGATAAAGAGCAAATAAGAGCGGCGGTTGATGTTGGGGCGCCAGTTATTGAATTGCACACGGGGTGTTAT
GCTGATGCTACCTCAGAAGAAAAGCAGCATTATGAATTGCAACGAATTAAAGAGGCAGCTGAATTTGCTGCCAGTTTGAA
CTTGGTAGTGAATGCAGGTCATGGGTTGCACTATCATAATGTTAAACCGATTGCAGCGATTAGGGAGTTAAATGAACTTA
ACATAGGACATGCCATTATTGCCAGAGCATTATTCTGTGGATTAAAAGAGGCAGTAAGACACATGCGCCAATTAATGCAG
GAAGCGAGACTTTATGTCAACGACTGA

Upstream 100 bases:

>100_bases
CTTTAGGTAGTGCCGTTTAAACTGATTGAAACAAGGTAACGTGAATAGCAGTTTGTCAAGAACTGCATTATACTCTGCAT
TTTATATTTTTTGGTGCAAA

Downstream 100 bases:

>100_bases
TGTCATTGTTGTACGTATCACTGGCGATTCTGGTGACGGAGTACAATTGGTAGGTGAACAATTAACCATTAGCGCGGCAT
TGACAGGACGTGATGTACGT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAM
LDFAEEILPEHACLVPEKREELTTEGGLDILTHRNVVERAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
EARLYVND

Sequences:

>Translated_248_residues
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAM
LDFAEEILPEHACLVPEKREELTTEGGLDILTHRNVVERAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
EARLYVND
>Mature_248_residues
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAM
LDFAEEILPEHACLVPEKREELTTEGGLDILTHRNVVERAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
EARLYVND

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=240, Percent_Identity=67.0833333333333, Blast_Score=321, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_LEGPA (Q3V7G1)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123336.1
- ProteinModelPortal:   Q3V7G1
- SMR:   Q3V7G1
- STRING:   Q3V7G1
- GeneID:   3117221
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1008
- LegioList:   lpp1008
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   LPNE297246:LPP1008-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27883; Mature: 27883

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: ACT_SITE 46-46 ACT_SITE 73-73 ACT_SITE 194-194 BINDING 10-10 BINDING 21-21 BINDING 48-48 BINDING 53-53 BINDING 103-103 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVR
CCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHCHHHCCCCCEEEEEHHHHHHHHHHHHH
LIKQVLQTRMNLELAVTEAMLDFAEEILPEHACLVPEKREELTTEGGLDILTHRNVVERA
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
VRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCYADATSEEKQHYELQRIKEAA
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
EFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
HHHHHHHHEEECCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EARLYVND
HHHHCCCC
>Mature Secondary Structure
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVR
CCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHCHHHCCCCCEEEEEHHHHHHHHHHHHH
LIKQVLQTRMNLELAVTEAMLDFAEEILPEHACLVPEKREELTTEGGLDILTHRNVVERA
HHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
VRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCYADATSEEKQHYELQRIKEAA
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
EFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
HHHHHHHHEEECCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EARLYVND
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA