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The map label for this gene is ksgA [H]

Identifier: 54295765

GI number: 54295765

Start: 3259523

End: 3260293

Strand: Reverse

Name: ksgA [H]

Synonym: lpl2855

Alternate gene names: 54295765

Gene position: 3260293-3259523 (Counterclockwise)

Preceding gene: 54295767

Following gene: 54295764

Centisome position: 97.45

GC content: 38.52

Gene sequence:

>771_bases
GTGAGGCATAGCCCACGTAAACGTTTTGGTCAAAATTTCTTACAAGACAAGTATATTATTAATGAGATTCTACGCGCTAT
CAATCCTTTGGCGGATGATAATATGCTGGAGATTGGCCCTGGTTTAGGCGCTTTAACTCAGCCATTATTGCAAAAACTGA
ATCGATTAACAGCCATTGAAATCGATACGGATTTACAAAGCTATTTAACCTGCTTGCCTGTATCTCAAGGCAAACTGAAT
TTAATTCCTGCTGATGCGTTAACCGTTGACTTTTGTCAATTCGGCCCCCACTTAAGAGTAGTGGGTAATTTACCTTATAA
TATTTCTACTCCATTGCTGATCTATTTATTGAAATTTATAACTTGTATTGATGACATGCATTTTATGTTACAAAAAGAAG
TTGTAGAACGAATAGCAGCAGCCCACGGCACTAAAGCCTATGGGCGACTAAGCGTGATGTTGCAGTATCATTGTGAGGTA
GAGTATTTATTTGATGTTCCTCCAGAAGCATTTGAGCCCAGGCCCAAGGTAGACTCAGCAATAGTTCGATTAACACCTCA
TCGCGTTTCTCCATTTGAGTCTGTAAACACTGAGAAACTGGAAAATATTGTTGCTAAAGCATTTGCTATGAGACGCAAAA
CTCTTACTAATAATTTGAAAGGGATTATCTCATTATCTCAACTCAATGATTTAGGCATTGATGGAGGTAAACGGCCAGAA
CAAATTTCGGTGGCGGAATATGTCCAACTAGCGAAATTTATTTCCAATTAG

Upstream 100 bases:

>100_bases
ACTAACCTACGGTAAAATTAACAGGCTTCCCCAATGGAGTATACCTTGTGATGTCAACAAGATTACTGAATTAATTTTAT
CCTAACATAGGTGATAGAAA

Downstream 100 bases:

>100_bases
TGTAAAATTATATGATAAGTTATTGTGGAGGTTGTCTTGTTTCATCGTCAGCGTAAAAGCTCTTTATCTGCTCAGTCCAA
GTCATTAAAAGATTTAATCC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIEIDTDLQSYLTCLPVSQGKLN
LIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEV
EYLFDVPPEAFEPRPKVDSAIVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
QISVAEYVQLAKFISN

Sequences:

>Translated_256_residues
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIEIDTDLQSYLTCLPVSQGKLN
LIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEV
EYLFDVPPEAFEPRPKVDSAIVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
QISVAEYVQLAKFISN
>Mature_256_residues
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIEIDTDLQSYLTCLPVSQGKLN
LIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFITCIDDMHFMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEV
EYLFDVPPEAFEPRPKVDSAIVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
QISVAEYVQLAKFISN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=281, Percent_Identity=32.7402135231317, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI7657198, Length=219, Percent_Identity=33.7899543378995, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=50.1945525291829, Blast_Score=256, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI25146882, Length=210, Percent_Identity=34.7619047619048, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=244, Percent_Identity=29.0983606557377, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324989, Length=218, Percent_Identity=27.0642201834862, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21358017, Length=218, Percent_Identity=34.8623853211009, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357273, Length=287, Percent_Identity=29.616724738676, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 28844; Mature: 28844

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHEEEE
IDTDLQSYLTCLPVSQGKLNLIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFI
ECCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
TCIDDMHFMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEVEYLFDVPPEAFEPRPKVDSA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCEEEEECCCCHHCCCCCCCCCH
IVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
HEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCCCCCCC
QISVAEYVQLAKFISN
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRHSPRKRFGQNFLQDKYIINEILRAINPLADDNMLEIGPGLGALTQPLLQKLNRLTAIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHEEEE
IDTDLQSYLTCLPVSQGKLNLIPADALTVDFCQFGPHLRVVGNLPYNISTPLLIYLLKFI
ECCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
TCIDDMHFMLQKEVVERIAAAHGTKAYGRLSVMLQYHCEVEYLFDVPPEAFEPRPKVDSA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEECCCEEEEECCCCHHCCCCCCCCCH
IVRLTPHRVSPFESVNTEKLENIVAKAFAMRRKTLTNNLKGIISLSQLNDLGIDGGKRPE
HEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHCCCCCCCCCC
QISVAEYVQLAKFISN
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA