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The map label for this gene is metG [H]

Identifier: 54295707

GI number: 54295707

Start: 3195077

End: 3197089

Strand: Reverse

Name: metG [H]

Synonym: lpl2795

Alternate gene names: 54295707

Gene position: 3197089-3195077 (Counterclockwise)

Preceding gene: 54295709

Following gene: 54295706

Centisome position: 95.56

GC content: 41.48

Gene sequence:

>2013_bases
ATGACTAGTGAACGAAAAATGTTAGTGACCAGTGCCCTGCCTTATGCTAATGGGCATTTGCATCTGGGACATTTGGTTGA
ACATATTCAAACGGATATTTGGGTCCGTACTCACAAAATGTTGGGTATTCAATGTATTAGCGTTTGTGGGGATGACGCTC
ATGGAACACCCATTATGCTGAAAGCAGAGCAGCTGGGAATTACTCCGGAAGCTTTAACCGCCGAAATTAAGTTAAGTCAT
GAAAAGGATTTTAAAGCCTTCGCCATTGATTATGATTATTACCACACTACTCATTCACCTGAAAATCAGGCATTGGCGAC
TACCATATTTGAACGTTTGCAGGCAGGCGGTGATATAGTCAAGAAAACAATTCGCCAGTTTTATGATCCCGTAAAGCAAA
TGTTTTTGCCGGACAGGTATGTGAAGGGAACTTGCCCCAAATGTGCTGCTGTCGATCAGTATGGCGATAATTGCGAGGTT
TGTGGCGCCACTTATTCGCCGACGGATTTGATTAACCCTGTTTCTGTTATATCGGGAGCCAGCCCTGTAGAAAAGGAATC
AGAGCATTATTTTTTTGATTTGCCGCGTTACGAAGAGTTACTTAAGGATTGGACAAGAAAGGGGCATTTACAAACCGAAG
TAACGAATAAACTCAGCGAATGGTTTGAGGCGGGTTTAAAGCAATGGGATATTTCTCGCGATGCTCCCTATTTTGGTTTC
CCTATTCCCGGTGTTCCGGATAAATACTTTTACGTTTGGTTAGATGCTCCCATCGGCTATATGGCAAGCTTTAAAAAGTA
TTGCGATGAGCGTGGCGTTTCTTTTGATGAGTTTTGGGATAAGACTTCAAAAACAGAATTATATCATTTTGTAGGCAAAG
ATATTGTGTATTTTCATGCTTTGTTCTGGCCTGCTATGTTAGCTGCCAGCGGTTTCAGAACACCTACTGCTGTGTATACC
CATGGGTTTTTAACGGTTGAGGGGCAGAAAATGTCCAAATCGCGCGGTACTTTTATAGAAGCACGCGCTTATCTGGCTCA
CTTGCATCCTGAATATCTGCGTTATTATTTCGCTGCCAAATTGAATGGTCGAGTAGACGATTTGGATTTGAATTTTGACG
ATTTTGTCAATAGGGTGAATGCGGATCTGGTTGGGAAGGTAGTGAATATTGCAAGCCGATGCGCGGGATTTATTAATAAG
CGTTTTGATAATCGGTTGAGCTCAGAGTTGAATGATCCAAAATTACACAACGATTTGCTGAGCGCAAGAGAGTCCGTGAT
TGACGCATTTGTTTCAAGAGACTATGCGCGAGCAATAAGACAAATCATGGATTGTGCTGACAAAGTCAATCAATACATCG
ATGCCAATAAACCTTGGGTATTGGCTAAAGATGAAAGTAAACTAAATGAAGTTCATGCCATATGTACAATGGGAATCAAT
TTATTCCGCATTTTAATAACCTATTTAAAACCAGTCTTGCCAATGATGGCCAAAGCTTCAGAAGAATTTTTAAATTCTGA
GCCTTTACACTGGGGTTCTATCGATAAGCCTTTGCTAAACCACCGTATCAATACTTTTAAGCCTTTGATGGTCAGAGTAG
AAAAAGAAAAAATTGAAGCGATGTTGGCTCAAAGCAAGGAATCTTTGATGACTACCCAAACAAAAGAAAATACGCCAGTT
GAGGACTCTAACTTAATCAGTATTGAGGATTTTGCCAAAGTTGATTTACGGATTGCAAAAATTGTCAATGCAGAACCTGT
GGAAGGCGCAGATAAACTAATGCGCTTGATTTTAGACTTGGGTGATGCTCAAAAGCAGGTTTTTGCCGGCATAAAAAAGG
CTTATGATGCTGAAGAGTTGATTGGGCGTTTGACTGTAATGGTTGCTAATTTGGAGCCCCGCACTATGCGTTTCGGTGTT
TCTGAAGGCATGGTATTGGCGGCAGGGGATGGACAGGGAATTTATTTATTACAGCCTGATGCTGGAGCACTTCCTGGCAT
GAAGGTGAAGTAA

Upstream 100 bases:

>100_bases
TAAATAACTACCAATAAAAACCTGTTTTCTATTGCAGCATTTCCATTGTCTTTGCGTTATAATTAGTCGCTTTATTATAG
GTGATGAGTATTCTATAACT

Downstream 100 bases:

>100_bases
TGGCCTCTGTTAAAGAAATTGATGCCCTATTGCCGCAAACCCAGTGTGGGGAATGCGGATATGCAGGTTGTTTGCCTTAT
GCTGAAGCGCTTGCCCAAGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSH
EKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
CGATYSPTDLINPVSVISGASPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKTSKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYT
HGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINK
RFDNRLSSELNDPKLHNDLLSARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLAQSKESLMTTQTKENTPV
EDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGV
SEGMVLAAGDGQGIYLLQPDAGALPGMKVK

Sequences:

>Translated_670_residues
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSH
EKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
CGATYSPTDLINPVSVISGASPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKTSKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYT
HGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINK
RFDNRLSSELNDPKLHNDLLSARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLAQSKESLMTTQTKENTPV
EDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGV
SEGMVLAAGDGQGIYLLQPDAGALPGMKVK
>Mature_669_residues
TSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHE
KDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVC
GATYSPTDLINPVSVISGASPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFP
IPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKTSKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTH
GFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKR
FDNRLSSELNDPKLHNDLLSARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGINL
FRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLAQSKESLMTTQTKENTPVE
DSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVS
EGMVLAAGDGQGIYLLQPDAGALPGMKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=27.2241992882562, Blast_Score=239, Evalue=9e-63,
Organism=Homo sapiens, GI24308436, Length=523, Percent_Identity=24.0917782026769, Blast_Score=146, Evalue=5e-35,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=60.0297176820208, Blast_Score=868, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=106, Percent_Identity=34.9056603773585, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=520, Percent_Identity=29.2307692307692, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=24.0704500978474, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71992781, Length=405, Percent_Identity=24.4444444444444, Blast_Score=125, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321704, Length=565, Percent_Identity=29.9115044247788, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=22.803738317757, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24655311, Length=567, Percent_Identity=29.9823633156966, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI21358483, Length=517, Percent_Identity=23.9845261121857, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75797; Mature: 75666

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIML
CCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIV
EHHHHCCCHHHHEEEEEECCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHCCHHHH
KKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHCCC
SPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKTSKTELYHFVGKDIVYFHA
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAK
HHHHHHHHHCCCCCCCEEEECCEEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELNDPKLHNDLL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHEEHHHHHHH
MLAQSKESLMTTQTKENTPVEDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDL
HHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPD
CHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCEEEEECCC
AGALPGMKVK
CCCCCCCCCC
>Mature Secondary Structure 
TSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIML
CCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIV
EHHHHCCCHHHHEEEEEECCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHCCHHHH
KKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHCCC
SPVEKESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKTSKTELYHFVGKDIVYFHA
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHH
LFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAK
HHHHHHHHHCCCCCCCEEEECCEEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELNDPKLHNDLL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SARESVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHEEHHHHHHH
MLAQSKESLMTTQTKENTPVEDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDL
HHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPD
CHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCEEEEECCC
AGALPGMKVK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA