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The map label for this gene is nth [H]

Identifier: 54295705

GI number: 54295705

Start: 3193835

End: 3194470

Strand: Reverse

Name: nth [H]

Synonym: lpl2793

Alternate gene names: 54295705

Gene position: 3194470-3193835 (Counterclockwise)

Preceding gene: 54295706

Following gene: 54295704

Centisome position: 95.48

GC content: 41.19

Gene sequence:

>636_bases
ATGAATAAACAAAAATCACAAGAAATTTTCAAACGTTTTCAAGAACAAAACCCTCATCCTACAACGGAGTTGCAATATAG
TACCCCCTTTGAGTTATTAATTGCCGTCATGCTTTCAGCCCAGGCAACAGACGTTAGTGTGAACAAAGCGACAGCCAAAC
TATTCCCGGCAGCCAATACCCCACAAGCCTTGCTGGATTTGGGATTAGATAGATTAAAAGATTACATTAAGTCCATAGGT
CTTTATAATAGCAAAGCACAAAATATCATGAAAACCTGTGAAATACTTATTGATCAGCATGAGGGACAAGTTCCTGGCCA
GAGAGAGGCGCTGGAAGCATTGCCTGGTGTTGGCCGAAAAACTGCAAATGTGGTTTTGAATACAGCATTTGGTCAGCCAA
CCATAGCGGTCGACACCCATATTTTCAGAGTGGTTAACCGGATTGGCATAGCGAAGGGTAATACACCGCTAGCAGTTGAG
CAGGAGTTATTAAAAAAAGTGCCCCGTGAGTTTTTACATGACGCTCATCACTGGTTAATATTACATGGCCGTTATGTCTG
TACAGCACGTAAACCACATTGCAAGACTTGCATCATTGAAGATCTTTGCGAATACAAAGACAAGAATTTACTTTAA

Upstream 100 bases:

>100_bases
AAGAAACTTTCAGCCCATGCGCAAGATCATACTCAAGACGTACAGGCAAAACAAAATTATATTCTTGAGGCTTTGGCTCG
GGTGAAAGCAAAAAAGACAT

Downstream 100 bases:

>100_bases
GACAAAGCCATAATGAAGGAAAATCAAACGATAATGAACAATCTATGCCTCATAAAAATGACTGGCTGTTGTATTTCAAA
TTCATTCTATCAAATCATCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPAANTPQALLDLGLDRLKDYIKSIG
LYNSKAQNIMKTCEILIDQHEGQVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVVNRIGIAKGNTPLAVE
QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL

Sequences:

>Translated_211_residues
MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPAANTPQALLDLGLDRLKDYIKSIG
LYNSKAQNIMKTCEILIDQHEGQVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVVNRIGIAKGNTPLAVE
QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL
>Mature_211_residues
MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPAANTPQALLDLGLDRLKDYIKSIG
LYNSKAQNIMKTCEILIDQHEGQVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVVNRIGIAKGNTPLAVE
QELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=32.2033898305085, Blast_Score=94, Evalue=7e-20,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=72.5961538461538, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI1789331, Length=184, Percent_Identity=24.4565217391304, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=34.2857142857143, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=28.735632183908, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.638418079096, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23722; Mature: 23722

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPAANT
CCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
PQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCEILIDQHEGQVPGQREALEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
TANVVLNTAFGQPTIAVDTHIFRVVNRIGIAKGNTPLAVEQELLKKVPREFLHDAHHWLI
HHHHEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LHGRYVCTARKPHCKTCIIEDLCEYKDKNLL
EECEEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKQKSQEIFKRFQEQNPHPTTELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPAANT
CCCHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
PQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCEILIDQHEGQVPGQREALEALPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
TANVVLNTAFGQPTIAVDTHIFRVVNRIGIAKGNTPLAVEQELLKKVPREFLHDAHHWLI
HHHHEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LHGRYVCTARKPHCKTCIIEDLCEYKDKNLL
EECEEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]