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The map label for this gene is nuoF [H]

Identifier: 54295613

GI number: 54295613

Start: 3082153

End: 3083430

Strand: Reverse

Name: nuoF [H]

Synonym: lpl2700

Alternate gene names: 54295613

Gene position: 3083430-3082153 (Counterclockwise)

Preceding gene: 54295614

Following gene: 54295612

Centisome position: 92.16

GC content: 43.04

Gene sequence:

>1278_bases
ATGGTTGAACTAAATCAAGTTTGTTACCGAACATTTCATTTACCAGAGCCCTGGTCGCTATCAACTTATGAAAGTATTGG
TGGCTATAGCGCATGGCGAAAAATTTTAAAAGAGCAAACATCACCTCAATTAATAATTGAAGAGTTGAAAACTTCTGCCT
TACGCGGACGAGGAGGGGCTGGATTCCCAACCGGTTTAAAATGGAGTTTTATGAATAGAAACTCCCCTGTTCAAAAATAC
GTAGTATGTAATTCTGATGAAGGTGAACCGGGTACTTGTAAGGACAGAGAGATTCTTACTTTAAATCCTCACCAATTAAT
CGAAGGAATGGCCATAGCTGGGTATGTTATGGGCGCTACGGTTGGTTACAACTACATAAGAGGAGAATTCTGGCTGCCTT
TTGAGCGTACTGAGGCTGCAATAAAAGAAGCATACGATGCTGGCTTGCTAGGTAAAAATATATTAGGCTCCGGCGTTGAT
TTTGATTTATATAATCATTTAGGAGCGGGAGCTTACATTTGCGGTGAAGAAACTGCATTGCTTAACTCGATTGAAGGAAA
AAAGGGAATGCCTCGTTTTAAACCACCATTCCCGGCAAATTATGGCTTGTATGGAAAACCGACAACCATAAATAATACGG
AAACATTTGCGTCTGTTCCGGTTATTATGGAAAAAGGTGGAGAATGGTTTTTAAAATTAGGCAAACCGAACAATGGCGGT
GCCAAATGCTTTAGTGTTAGCGGCCATGTTAACAAACCTGGTAACTATGAAATCCCCTTGGGCACTCCATTCAAAACGCT
GCTTGATCTGGCTGGCGGCGTCATTAAAGGCCGAAAAATCAAAGCAGTGATTCCTGGTGGTTCCTCTATGCCAGTGCTCC
CGGGTGACGTCATGCTGGGGCTGGATATGGATTATGACAGTATACAGAAAGCCGGCTCAGGCTTGGGTTCAGGCGCAGTT
ATTATCATGGATGAAACCACCTGCATGGTGGATGCTTTGTATCGTATTTCTGAGTTCTACATGGATGAATCCTGTGGACA
ATGCACGCCTTGTCGAGAAGGCACAGGCTGGTTAGTCAGGTTATTACACAGAATTATAAACGGTCATGGCGAGCCGGGAG
ACGTGGAGAAGTTAGTGAATGTGGCGAATAACATAGAGGGAAGAACCATTTGTGCATTAGGCGAGGCAGCAGCTTGGCCA
GTACAGAGTTTTGTTAAACATTTCTATGATGAATTTGAATATTTCATAAAGCATAAACGCTCGATCGTCGCAGCATAA

Upstream 100 bases:

>100_bases
GCCAATGATGCAAGTTGATAAAGATTATCACGAAAATTTGACTGCTGAATCTATAGATAAAGTATTGGAACAGTACCAAT
AGAAAATAAAGGTGTTTGCC

Downstream 100 bases:

>100_bases
TTAAGAAGGTTAGACCATGGCTACTATTGAAATCGACGGTAAAACATTTGAAGCAGAAAACGGTAAAATGATTATAGAGG
TTGCTGATGAGGCGGGCATC

Product: NADH dehydrogenase I chain F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQLIIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKY
VVCNSDEGEPGTCKDREILTLNPHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFASVPVIMEKGGEWFLKLGKPNNGG
AKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIKGRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAV
IIMDETTCMVDALYRISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICALGEAAAWP
VQSFVKHFYDEFEYFIKHKRSIVAA

Sequences:

>Translated_425_residues
MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQLIIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKY
VVCNSDEGEPGTCKDREILTLNPHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFASVPVIMEKGGEWFLKLGKPNNGG
AKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIKGRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAV
IIMDETTCMVDALYRISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICALGEAAAWP
VQSFVKHFYDEFEYFIKHKRSIVAA
>Mature_425_residues
MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQLIIEELKTSALRGRGGAGFPTGLKWSFMNRNSPVQKY
VVCNSDEGEPGTCKDREILTLNPHQLIEGMAIAGYVMGATVGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVD
FDLYNHLGAGAYICGEETALLNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFASVPVIMEKGGEWFLKLGKPNNGG
AKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIKGRKIKAVIPGGSSMPVLPGDVMLGLDMDYDSIQKAGSGLGSGAV
IIMDETTCMVDALYRISEFYMDESCGQCTPCREGTGWLVRLLHRIINGHGEPGDVEKLVNVANNIEGRTICALGEAAAWP
VQSFVKHFYDEFEYFIKHKRSIVAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=399, Percent_Identity=52.8822055137845, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI260656005, Length=399, Percent_Identity=52.8822055137845, Blast_Score=424, Evalue=1e-119,
Organism=Escherichia coli, GI1788620, Length=419, Percent_Identity=44.8687350835322, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17531727, Length=372, Percent_Identity=53.494623655914, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI19920794, Length=372, Percent_Identity=52.6881720430108, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45550452, Length=405, Percent_Identity=47.9012345679012, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24653819, Length=410, Percent_Identity=36.5853658536585, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24653817, Length=352, Percent_Identity=37.2159090909091, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46466; Mature: 46466

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQLIIEELKTSALRGRGGA
CCCHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLNPHQLIEGMAIAGYVMGAT
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
VGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVDFDLYNHLGAGAYICGEETAL
HCCCEECCCEECCHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHCCCCEEEECCHHHH
LNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFASVPVIMEKGGEWFLKLGKPNNGG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCEEEEEECCCCCCC
AKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIKGRKIKAVIPGGSSMPVLPGDVMLG
CEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCEECCCEEEEEECCCCCCCCCCCCEEEE
LDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALYRISEFYMDESCGQCTPCREGTGWLVR
ECCCHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LLHRIINGHGEPGDVEKLVNVANNIEGRTICALGEAAAWPVQSFVKHFYDEFEYFIKHKR
HHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
SIVAA
HHCCC
>Mature Secondary Structure
MVELNQVCYRTFHLPEPWSLSTYESIGGYSAWRKILKEQTSPQLIIEELKTSALRGRGGA
CCCHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GFPTGLKWSFMNRNSPVQKYVVCNSDEGEPGTCKDREILTLNPHQLIEGMAIAGYVMGAT
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHH
VGYNYIRGEFWLPFERTEAAIKEAYDAGLLGKNILGSGVDFDLYNHLGAGAYICGEETAL
HCCCEECCCEECCHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHCCCCEEEECCHHHH
LNSIEGKKGMPRFKPPFPANYGLYGKPTTINNTETFASVPVIMEKGGEWFLKLGKPNNGG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCEEEEEECCCCCCC
AKCFSVSGHVNKPGNYEIPLGTPFKTLLDLAGGVIKGRKIKAVIPGGSSMPVLPGDVMLG
CEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCEECCCEEEEEECCCCCCCCCCCCEEEE
LDMDYDSIQKAGSGLGSGAVIIMDETTCMVDALYRISEFYMDESCGQCTPCREGTGWLVR
ECCCHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LLHRIINGHGEPGDVEKLVNVANNIEGRTICALGEAAAWPVQSFVKHFYDEFEYFIKHKR
HHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
SIVAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA