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The map label for this gene is dotB [H]

Identifier: 54295516

GI number: 54295516

Start: 2969519

End: 2970652

Strand: Direct

Name: dotB [H]

Synonym: lpl2603

Alternate gene names: 54295516

Gene position: 2969519-2970652 (Clockwise)

Preceding gene: 54295515

Following gene: 54295517

Centisome position: 88.76

GC content: 42.95

Gene sequence:

>1134_bases
ATGTCTATGGATAATATTAATTTAATGCCCGATGAGCCAACTCGTTTCACTCCAGTATTCATGGACAGAATGCTGGAGCA
TGCAGAAAGCCTGAATGCTTCAGATATAACAATCCAAACTGGCGAACCCATTTTTGCAGAGGTTTATGGAAGACTCCTTA
AAATTACAAATCGACGTCTTTCCAATACAGAATTGGGCGACCTTATAAATTCCATTTATGGCCCGAATGCCACGACACAA
TTACTTTCCGGTAAAGATATAGATACTCATTATGAGTTTCGGCCCAACCGTGGTGTACGCTATCGATATAGGGTTAACGC
GACTGCCTGCCTGGTAGAAGGTCATGATGCCATTCAGATCACATTAAGAACTATTCCGACCACACCACCCAAACTCAGTA
CCATGAACCTCCCTGATAACATTATTGAAGCGATTGCTCCACAGGAAGGAATCGTGTTTATCACAGGCGCTACCGGTTCG
GGCAAATCGACATTGTTGGCGTCCATTATTCGAGAGCTTATTGAAACATCAGACTCAAACCGCAAAGTATTAACTTACGA
ATCCCCAATAGAATTCGTCTATGATGAAATTGAAACAATTTCCGCTGTAGTAAGCCAATCAGAGATTCCAAGACACTTAC
CCAACTTTGCTGATGGAGTAAGAAACGCATTAAGACGAAAGCCGAGATTAATTATGGTGGGTGAATGTCGTGATGCAGAA
ACCATCAGTGCTGCTCTTGAAGCTGCTTTAACAGGACACCCGGTCTACACAACCCTGCATACTTCTGGTGTTGCAGAAAC
GATGCGACGTCTGGTCACGTCTTTTAGCGGGGAAGAGCGTTTGGGAAGAACCATCGATATTCTTGAAACAATTAGGCTTT
GCATTTGGCAAAAACTGGTCCCAACCGTTGATGAAAGACGTGTAGCCCTAAGGGAGTATCTGGTATTCGATGAAGAAGTG
AGAGATATTCTATTGGAGGGAGACCCTAACGAAGTGACTTCAGCCACTCGTAAGTTAGTGAGGCAAAAAGGGCAGTTAAT
GACTTGGGACGCAAAAATGAAATTTGAACAGGGGATTATCAGTGAGCGAGTTTATAAATTAATCATTGCTGGGGCCAAAG
AATATCAACAATAA

Upstream 100 bases:

>100_bases
ATGCTCTCGAACGTTTCAAAAATATGGAAAAACTGGCAAATCAGGCTAAAATTGTTATAACGAACAAATCCTGGCAACCT
ATTATAGCACCAGTAAGTTA

Downstream 100 bases:

>100_bases
TCAAAATTAATCTGCTTGCACTTGCCATAGAAAATCATTGATATACCACATATAAAACAATCAAGATCAAGGATTTGATT
CACGATGTTTCACAGGAAAT

Product: defect in organelle trafficking protein DotB (ATPase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQ
LLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGS
GKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAE
TISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEV
RDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ

Sequences:

>Translated_377_residues
MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQ
LLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGS
GKSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAE
TISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEV
RDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ
>Mature_376_residues
SMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRLSNTELGDLINSIYGPNATTQL
LSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQITLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSG
KSTLLASIIRELIETSDSNRKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAET
ISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLVPTVDERRVALREYLVFDEEVR
DILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGIISERVYKLIIAGAKEYQQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=216, Percent_Identity=38.4259259259259, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789723, Length=150, Percent_Identity=39.3333333333333, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786296, Length=133, Percent_Identity=39.0977443609023, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42456; Mature: 42325

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCC
SNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQI
CCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCCCCEEEEEECCEEEEEECCCEEEE
TLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASIIRELIETSDSN
EEEECCCCCCCCEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
RKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHH
TISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLV
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHC
PTVDERRVALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGII
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
SERVYKLIIAGAKEYQQ
HHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
SMDNINLMPDEPTRFTPVFMDRMLEHAESLNASDITIQTGEPIFAEVYGRLLKITNRRL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCC
SNTELGDLINSIYGPNATTQLLSGKDIDTHYEFRPNRGVRYRYRVNATACLVEGHDAIQI
CCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCCCCEEEEEECCEEEEEECCCEEEE
TLRTIPTTPPKLSTMNLPDNIIEAIAPQEGIVFITGATGSGKSTLLASIIRELIETSDSN
EEEECCCCCCCCEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCC
RKVLTYESPIEFVYDEIETISAVVSQSEIPRHLPNFADGVRNALRRKPRLIMVGECRDAE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCHH
TISAALEAALTGHPVYTTLHTSGVAETMRRLVTSFSGEERLGRTIDILETIRLCIWQKLV
HHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHC
PTVDERRVALREYLVFDEEVRDILLEGDPNEVTSATRKLVRQKGQLMTWDAKMKFEQGII
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHH
SERVYKLIIAGAKEYQQ
HHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]