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The map label for this gene is rpoD [H]

Identifier: 54295203

GI number: 54295203

Start: 2601678

End: 2603543

Strand: Direct

Name: rpoD [H]

Synonym: lpl2283

Alternate gene names: 54295203

Gene position: 2601678-2603543 (Clockwise)

Preceding gene: 54295202

Following gene: 54295206

Centisome position: 77.76

GC content: 42.18

Gene sequence:

>1866_bases
ATGACCATGAATGACCAAGATCAGCAAAGCTCACAAATTACGAAAGTCATTAGCCTGGGTAAAGAACAAGGTTATTTGAC
TTACAGTCAAATTAATGACTTATTACCTAACATTGTGGATACAGAGCATTTTGATGTAATCATTAGCATGCTGGAAGGCA
TGAACATCAAAGTTTTTGAGCTTCCACCCAGTGAAGATGAACTTGCGCTCTTATTAAATACTGAGGAAGTCCCAGATATT
GAAGAAGCAGCCATGGTACTCGCCTCTGTGGATAAAGAAACAGGTAGAACAACTGACCCGGTTCGTATGTATATGCGCGA
AATGGGCACAGTAGAACTGCTGACTCGTGAAGGTGAAATCCGCATTGCCAAACGCATTGAAGAAGGAATTTACCAGGTTC
TAAAATCCCTGGCGCATTATCCGGAAACAGTGCAACTGGTTCTTGAAGACTATGATCGTTTCCTTGCCGAAGAAATTCGC
CTTAATGAAATCATTAGTGGTTTTGCGGATGTTGATGAAGAAATAGCACCTGCCACTAACATTGGCTCCATGCTGGATGA
AGAGCAACAGGAAGAAATACTTCTTGATGTGGATGAGGAAGATGAAGACGGCGAAGGTGGCATATCTGATGTGGATGATG
GACCCAATCCTGAGCAAGCGAAGATTTACTTTGATGAACTGCGTGAAGCCTTTGAAAAGGCAATGAAAGCCCTCAAAGAA
CATGGCCGTACGAATAAAAAAACGGTCGAGTTGCTTGATTCCATGTCAGAATCTTTTCTAAAACTCAAATTGACTTCCAG
GCAAGTTGATAAATTAACTCGTCATTTTCGCCAATTGCGAAATCATATCCGTGAATTTGAGAGAAGCATTATGCGTCTTT
GCATCGAAAAAGCACGCATCCCCAGAAAATTGTTTATAGATACCTTCCCAGGTCAGGAAACAGATCTAGGCTGGTTAGAA
ACCATTATCAGCAAACATGCCAAAAAACTCGATCTTCAGCGTATAGAAGAAAATCGGGAAGAAATACTGCGCTTACAATC
CAAATTGGCCTCTTTTGAAATCGAATATGGTTTAACCATTAGTGAAATCAAAGACATTAACCGCAAGATGTCTATTGGCG
AAGCCAAAGCAAGACGCGCTAAAAAAGAAATGGTCGAGGCTAACTTGCGTCTCGTGATTTCTATCGCCAAAAAATACACC
AACCGCGGTTTGCAGTTTCTTGATTTGATCCAGGAAGGCAACATTGGCTTGATGAAAGCAGTCGATAAATTCGAATACCG
TCGTGGCTATAAGTTTTCTACCTATGCGACTTGGTGGATTAGACAAGCGATTACACGCTCCATAGCCGATCAGGCGCGGA
CTATTCGTATTCCGGTTCATATGATTGAAACAATCAATAAGCTCAATCGTATTTCTCGCCAAATTTTGCAAGAAACTGGC
AGGGAAGCGACACCGGAAGAATTGGCCGAAAAAATGGAGCTAAGTGAAGATAAAATTCGCAAAGTTCTAAAGATAGCCAA
AGAGCCTATTTCCATGGAAACTCCTGTCGGTGATGATGACGACTCTCATCTGGGTGATTTTATTGAAGACAACAACATTG
AATCGCCAATCGATATGGCTACAGCGGAAGGTTTAAGAGAAGCCACTTTAGAAATACTGGAAACCTTGACCCCACGAGAA
GCCAAGGTTCTGCGTATGCGTTTTGGTATCGAAATGAATACAGACCATACCCTGGAAGAAGTTGGTAAACAATTTGACGT
AACCCGCGAGCGTATTCGCCAAATCGAAGCAAAAGCACTACGCAAATTGCGCCACCCTTCACGTTCAGAAAAGCTAAGAA
GCTTCCTTGAAGGAGACGAAGGTTAA

Upstream 100 bases:

>100_bases
AAATTGAAAAATAAAAATACTGAAATACTGGTATGTTGCAGCTACCCAGTTTATAATTTAAAACTTTTTGTAATTTCCCA
TTGTTCCAACAGAAGTGCCT

Downstream 100 bases:

>100_bases
TTTAATGACTGTTTTTACAAACAGTCATTAAAAAAATAAAAAAATAGTAGAACATTAAAGAAATTAAGTTTAAAATGACC
CCCTCTCCGGGCCCATAGCT

Product: RNA polymerase sigma factor rpoD (sigma-70)

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDI
EEAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKIYFDELREAFEKAMKALKE
HGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLGWLE
TIISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYT
NRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETG
REATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPRE
AKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG

Sequences:

>Translated_621_residues
MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDI
EEAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIR
LNEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKIYFDELREAFEKAMKALKE
HGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLGWLE
TIISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYT
NRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETG
REATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPRE
AKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG
>Mature_620_residues
TMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFELPPSEDELALLLNTEEVPDIE
EAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEIRIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRL
NEIISGFADVDEEIAPATNIGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKIYFDELREAFEKAMKALKEH
GRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARIPRKLFIDTFPGQETDLGWLET
IISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTISEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTN
RGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETGR
EATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMATAEGLREATLEILETLTPREA
KVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEKLRSFLEGDEG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=611, Percent_Identity=63.1751227495908, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=39.1791044776119, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.9256198347107, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=30.1980198019802, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 71693; Mature: 71562

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFE
CCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEE
LPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEI
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCH
RIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLNEIISGFADVDEEIAPATN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKIYFDELREAFEKAMKALKE
HHHHHCHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRKLFIDTFPGQETDLGWLETIISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTI
CHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCH
SEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCC
REATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMA
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHH
TAEGLREATLEILETLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKAL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKLRHPSRSEKLRSFLEGDEG
HHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMNDQDQQSSQITKVISLGKEQGYLTYSQINDLLPNIVDTEHFDVIISMLEGMNIKVFE
CCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEE
LPPSEDELALLLNTEEVPDIEEAAMVLASVDKETGRTTDPVRMYMREMGTVELLTREGEI
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCH
RIAKRIEEGIYQVLKSLAHYPETVQLVLEDYDRFLAEEIRLNEIISGFADVDEEIAPATN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IGSMLDEEQQEEILLDVDEEDEDGEGGISDVDDGPNPEQAKIYFDELREAFEKAMKALKE
HHHHHCHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HGRTNKKTVELLDSMSESFLKLKLTSRQVDKLTRHFRQLRNHIREFERSIMRLCIEKARI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRKLFIDTFPGQETDLGWLETIISKHAKKLDLQRIEENREEILRLQSKLASFEIEYGLTI
CHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCH
SEIKDINRKMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
VDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQILQETG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCC
REATPEELAEKMELSEDKIRKVLKIAKEPISMETPVGDDDDSHLGDFIEDNNIESPIDMA
CCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHH
TAEGLREATLEILETLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKAL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RKLRHPSRSEKLRSFLEGDEG
HHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]