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The map label for this gene is dnaG [H]

Identifier: 54295202

GI number: 54295202

Start: 2599855

End: 2601591

Strand: Direct

Name: dnaG [H]

Synonym: lpl2282

Alternate gene names: 54295202

Gene position: 2599855-2601591 (Clockwise)

Preceding gene: 54295201

Following gene: 54295203

Centisome position: 77.71

GC content: 39.32

Gene sequence:

>1737_bases
ATGTCTGGCTTAATCCCACAGCCATTCATTGATGATCTATTAAACCGCACTGATTTGGTGGAATTAATAGACGGATATGT
TCCTTTGAAAAAAAGAGGCAACAGCCATATTGCATGTTGCCCTTTCCATAATGAGAAAACACCATCATTCAATGTCGTTG
CAAAAAAGCAATTTTATCATTGTTTTGGTTGCGGCGCTTCAGGTAACGCCATCAGTTTTGTAATGAATTATCTGAATCAA
GGTTTTACGGATGCCGTGGAAACCCTTGCGACACGCTTGGGTTTAAGTGTCCCAAGGGATGGTACAGGAGAGAAACATAA
ATCATCTCTCAATCTCTATAATCTCATGTCAGAAGTCAGCCAATATTACCAAAAAAAATTAAAATATAATGGACAAGTGG
CAATTGACTACCTTCGCAACCGAGGTCTTAGTGGGGAAATTGCCAAACGATATCATTTAGGCTATGCACCTGAAGGTTGG
CATAACCTGGAAAAAACTTTCCCAAACAATCAGCGTGAACTATTGAGTACCGGTATGCTCATTAAAAGCGATGAGGGTAA
AATTTATGACAGATACCGTAATCGAATTATGTTCCCCATCCATGACAGAAACGGTCGTGTGATTGGTTTTGGCGGACGTC
TACTCGATAAAGATCAAAAACCCAAATACCTTAACTCGCCTGAAACAGTACTTTTTCAAAAAAGTCGTGAGTTATATGGC
TTACATCAAGTCCTGTCTCAGCAGAAAAATCCCGACAGTATTATTGTTGTTGAAGGTTATATGGATGTCATTGCCTTGGC
ACAACATGGCATTTTTAATGTGGTGGCAACATTGGGAACTGCCACCAGCACATTCCATATACAACTATTGGCAAAGCATA
CCAAACATCTCATTTTTTGCTTTGATGGTGATGCGGCAGGTAAACAAGCAGCCTGGCGAGCATTGGAGAGCAGTTTACCG
CATTTGAATGCCGGATTAGATGCCGGATTTATTTTCTTGCCGAATGAACATGACCCGGATAGCCTGGTAAGAAATGAAGG
AAAAAACGGTTTCCTCGAGCTCTTGCAACAAGCAACACCTCTCCATCGCTTTTTCTTTGATACTTTAAGTAAAGACATTA
ATTTGTCAAACCCTGCAGGAAAAACACAGCTTATCAACGCAGTGAAACCCTATTTGCAAAAAATGGTTGAGGGCTCATAC
AAACAGTTGCTTATTGATGACCTGTCACGCTTAACCCATATTGAAAGCCATCGGCTAACTAATTTGATTACTGATAAATC
GGAAAGCAAGCCGGAAGTACAAGCTGCTATTTCTCGCTCGCCTCTACGTATTGCTATCGCGCTCCTCTTACAAAAACCAG
AAATTTATCCCGCAGCAATACAACAAATCAATCCCGATCTGCTCAATGGGCAAGAGCATCATATTTTATTGAAATTATTG
CAACGATTGAAAGACAATCCCAATGCCAATACTGCCACACTCATTGAATATTGGAGGAATACCCGCTATTTTGAATCTAT
TATTAAATTAGCAGCATGGGATCATAATGTCCCCGAACCGGAACTGATTAATGAATTTATTGATACTGTATTGCTTTTAC
AAAAACAAAGTAATCGTGAGGCATTAATAGAACAATATATAAAAAAATCTCAACAAAAGGGGTTAACGAAAGAAGAGCAA
ATTATCTTGCAAAATTTGATAAAGGAAAGACATGCCCAAGCCGAAATTGAAAAATAA

Upstream 100 bases:

>100_bases
TGGGTAAAGTCATGGCTATCCTTAAGCCAAAATTACAAGGACGCGCGGACATGGCCCAAGTCAGCACCCGGATAAAGGCG
AAATTAAGCTGAGTTCCAAT

Downstream 100 bases:

>100_bases
AAATACTGAAATACTGGTATGTTGCAGCTACCCAGTTTATAATTTAAAACTTTTTGTAATTTCCCATTGTTCCAACAGAA
GTGCCTATGACCATGAATGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQ
GFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
HNLEKTFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRLLDKDQKPKYLNSPETVLFQKSRELYG
LHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLP
HLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSY
KQLLIDDLSRLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLLQKPEIYPAAIQQINPDLLNGQEHHILLKLL
QRLKDNPNANTATLIEYWRNTRYFESIIKLAAWDHNVPEPELINEFIDTVLLLQKQSNREALIEQYIKKSQQKGLTKEEQ
IILQNLIKERHAQAEIEK

Sequences:

>Translated_578_residues
MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQ
GFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGW
HNLEKTFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRLLDKDQKPKYLNSPETVLFQKSRELYG
LHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLP
HLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSY
KQLLIDDLSRLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLLQKPEIYPAAIQQINPDLLNGQEHHILLKLL
QRLKDNPNANTATLIEYWRNTRYFESIIKLAAWDHNVPEPELINEFIDTVLLLQKQSNREALIEQYIKKSQQKGLTKEEQ
IILQNLIKERHAQAEIEK
>Mature_577_residues
SGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYHCFGCGASGNAISFVMNYLNQG
FTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVSQYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGWH
NLEKTFPNNQRELLSTGMLIKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRLLDKDQKPKYLNSPETVLFQKSRELYGL
HQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFCFDGDAAGKQAAWRALESSLPH
LNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATPLHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSYK
QLLIDDLSRLTHIESHRLTNLITDKSESKPEVQAAISRSPLRIAIALLLQKPEIYPAAIQQINPDLLNGQEHHILLKLLQ
RLKDNPNANTATLIEYWRNTRYFESIIKLAAWDHNVPEPELINEFIDTVLLLQKQSNREALIEQYIKKSQQKGLTKEEQI
ILQNLIKERHAQAEIEK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=594, Percent_Identity=42.7609427609428, Blast_Score=458, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 65641; Mature: 65510

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYH
CCCCCCCHHHHHHHCHHHHHHHHHCCCCHHHCCCCCEEEECCCCCCCCCCEEEEHHHHHH
CFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVS
EECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGWHNLEKTFPNNQRELLSTGML
HHHHHHHCCCCCEEEHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHCCCE
IKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRLLDKDQKPKYLNSPETVLFQKSRELYG
EECCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFC
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHEECCCCEEEEE
FDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATP
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHCH
LHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSYKQLLIDDLSRLTHIESHRLT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NLITDKSESKPEVQAAISRSPLRIAIALLLQKPEIYPAAIQQINPDLLNGQEHHILLKLL
HHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCHHHHCCCHHHHHHHHH
QRLKDNPNANTATLIEYWRNTRYFESIIKLAAWDHNVPEPELINEFIDTVLLLQKQSNRE
HHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHH
ALIEQYIKKSQQKGLTKEEQIILQNLIKERHAQAEIEK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLIPQPFIDDLLNRTDLVELIDGYVPLKKRGNSHIACCPFHNEKTPSFNVVAKKQFYH
CCCCCCHHHHHHHCHHHHHHHHHCCCCHHHCCCCCEEEECCCCCCCCCCEEEEHHHHHH
CFGCGASGNAISFVMNYLNQGFTDAVETLATRLGLSVPRDGTGEKHKSSLNLYNLMSEVS
EECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QYYQKKLKYNGQVAIDYLRNRGLSGEIAKRYHLGYAPEGWHNLEKTFPNNQRELLSTGML
HHHHHHHCCCCCEEEHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHCCCE
IKSDEGKIYDRYRNRIMFPIHDRNGRVIGFGGRLLDKDQKPKYLNSPETVLFQKSRELYG
EECCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LHQVLSQQKNPDSIIVVEGYMDVIALAQHGIFNVVATLGTATSTFHIQLLAKHTKHLIFC
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHEECCCCEEEEE
FDGDAAGKQAAWRALESSLPHLNAGLDAGFIFLPNEHDPDSLVRNEGKNGFLELLQQATP
ECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHHHHCH
LHRFFFDTLSKDINLSNPAGKTQLINAVKPYLQKMVEGSYKQLLIDDLSRLTHIESHRLT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
NLITDKSESKPEVQAAISRSPLRIAIALLLQKPEIYPAAIQQINPDLLNGQEHHILLKLL
HHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCHHHHCCCHHHHHHHHH
QRLKDNPNANTATLIEYWRNTRYFESIIKLAAWDHNVPEPELINEFIDTVLLLQKQSNRE
HHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHH
ALIEQYIKKSQQKGLTKEEQIILQNLIKERHAQAEIEK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA