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The map label for this gene is metG [H]

Identifier: 54294866

GI number: 54294866

Start: 2183318

End: 2184877

Strand: Direct

Name: metG [H]

Synonym: lpl1945

Alternate gene names: 54294866

Gene position: 2183318-2184877 (Clockwise)

Preceding gene: 54294865

Following gene: 54294867

Centisome position: 65.26

GC content: 36.92

Gene sequence:

>1560_bases
ATGACTAATAGCAAAAAGTATTTACTTATCCCCTCAATCCCGACTCCCAATGGTCGCTTGCATTTAGGTCATATTGGCGG
TCCTTTCTTGAGTGCTGATATTTTAGCAAGATATTTAAGGACACGCGGACATTCAGCCTGGGTTATTTCTGGAACAGATA
GCTTTGAATCCTATGCCGCAGGAAAAGCAGAAGAAGAACATCGTAGTCCGGAAGAAACATGCAATTATTACCATCCATTA
ATTGAAGAGGATTTAAGATTAATGGGTATCCAAATGGATCTCTTCATTAATCCGCTAAAAGCACCGTGGAAATATCTCTA
CCAGCACTGGCACCACAACATTTACAATCAGCTAATTAATAATGGAACTACACTAGTCATTAAAGAGAAGTTTCCCTGGG
ATGAGGACAGTCAACGGTATCTATCAGGTTATTGGCTCAAAGGCGATTGTCCAATTTGCAGGAAAAAAACAGCCAGTTAT
TTTTGTGAGAATTGCGGGACACATTTTCGTCCTGAAGAACTGATTCAGGAGGATGCTTTTAATCAGAAAGAAGTAGAAAA
TATTTTTTTAAAACTGCCGAACGGCATCAATTTAAGTTCCAAAGGCGTCAGCAAAGAAATTCAAGCCATTTTCCATAACT
ATTTAGAACGACAAAACCAAATCTTGAGATTAACAACTTTTTGTGATTGGGGTTTGGCTGATCCTAATGATAAGACAAGA
ACATTATTTAGTTATGGTTTTGTATTTGCCTATTTCTTAATGATTGGAGAAATCGCTGGTCAATTAATCGGTATCAATAG
AAATGCTTTTGCCTCCGATTCAGACGTAATCACTATATCCAGTTTTGGTATCGATAATTCAATACCTTTTTTAAGTAGCT
CCATAGGTGTTAGTTCTGCTTGCCCTCAATACAAGCCCTTTGATTATTATCTTGTTAATTATTTTTATTATTTGGATGGG
AACAAATTTTCCACTAGTCGGCAACATATGATCGGAGTAAAGGATATTATCACCTGCAAGGAACTTTCAAGTGATCTTGT
CCGACTTTTCCTTGCTGCAATTGACGTGCGCCATCAAACCGGCGACTTTCTCACCGATGAATTTATTTATTATTATAATC
AAACCATTGACTGGCTTGAGGAATTGGTCATAACGGGCATACAAAACATAGCTCCACCAACAGTTTGTGATGATTTTTTT
AAAGATGAGTTAAATGAACTTTTGAAGACTCAAGAGTGTGCCTTGCAGCCTGAAAATTACTTACCACATCTCGCAGTAAA
TGCAATTAATCATTGGATCATTCAAGGAAAACAACTGAATCAGGACTCAAAGCACTATTTTTGGTGGTTAAAAGGATTAA
GTCTGGTGATTTATCCTTTTATGCCTAATCTTGGACAGACTATTTGGGGATCCTTAGGGTATGAAAACTCACCAAGGATA
AAAGATTTTTTTGCTCTCCCCCTGCATTCTATACAAAAAAATGTGCCAATAAATCGAAATCGAATTTCTCAAGAATTAAT
GTTTATCAAGGAGGAGCATGATGATGAAATCATTGCGTAA

Upstream 100 bases:

>100_bases
GATAAAGAAAGAGCGCTGAAAATATCACAAGAAGGCTGGGAAAATTCTCTCGAAATCATGGCGAGCATTATAAAAAGCAC
AGGCATTGGGACATTAAAAA

Downstream 100 bases:

>100_bases
TACTGAGGTTGTTGATGTAATCGGAGTTGGCATTGGACCCTCCAATTTAAGTCTCGCTGCTTTACTATCACCGATAAAAA
ACATTTCCAGTTGTTTTTAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTNSKKYLLIPSIPTPNGRLHLGHIGGPFLSADILARYLRTRGHSAWVISGTDSFESYAAGKAEEEHRSPEETCNYYHPL
IEEDLRLMGIQMDLFINPLKAPWKYLYQHWHHNIYNQLINNGTTLVIKEKFPWDEDSQRYLSGYWLKGDCPICRKKTASY
FCENCGTHFRPEELIQEDAFNQKEVENIFLKLPNGINLSSKGVSKEIQAIFHNYLERQNQILRLTTFCDWGLADPNDKTR
TLFSYGFVFAYFLMIGEIAGQLIGINRNAFASDSDVITISSFGIDNSIPFLSSSIGVSSACPQYKPFDYYLVNYFYYLDG
NKFSTSRQHMIGVKDIITCKELSSDLVRLFLAAIDVRHQTGDFLTDEFIYYYNQTIDWLEELVITGIQNIAPPTVCDDFF
KDELNELLKTQECALQPENYLPHLAVNAINHWIIQGKQLNQDSKHYFWWLKGLSLVIYPFMPNLGQTIWGSLGYENSPRI
KDFFALPLHSIQKNVPINRNRISQELMFIKEEHDDEIIA

Sequences:

>Translated_519_residues
MTNSKKYLLIPSIPTPNGRLHLGHIGGPFLSADILARYLRTRGHSAWVISGTDSFESYAAGKAEEEHRSPEETCNYYHPL
IEEDLRLMGIQMDLFINPLKAPWKYLYQHWHHNIYNQLINNGTTLVIKEKFPWDEDSQRYLSGYWLKGDCPICRKKTASY
FCENCGTHFRPEELIQEDAFNQKEVENIFLKLPNGINLSSKGVSKEIQAIFHNYLERQNQILRLTTFCDWGLADPNDKTR
TLFSYGFVFAYFLMIGEIAGQLIGINRNAFASDSDVITISSFGIDNSIPFLSSSIGVSSACPQYKPFDYYLVNYFYYLDG
NKFSTSRQHMIGVKDIITCKELSSDLVRLFLAAIDVRHQTGDFLTDEFIYYYNQTIDWLEELVITGIQNIAPPTVCDDFF
KDELNELLKTQECALQPENYLPHLAVNAINHWIIQGKQLNQDSKHYFWWLKGLSLVIYPFMPNLGQTIWGSLGYENSPRI
KDFFALPLHSIQKNVPINRNRISQELMFIKEEHDDEIIA
>Mature_518_residues
TNSKKYLLIPSIPTPNGRLHLGHIGGPFLSADILARYLRTRGHSAWVISGTDSFESYAAGKAEEEHRSPEETCNYYHPLI
EEDLRLMGIQMDLFINPLKAPWKYLYQHWHHNIYNQLINNGTTLVIKEKFPWDEDSQRYLSGYWLKGDCPICRKKTASYF
CENCGTHFRPEELIQEDAFNQKEVENIFLKLPNGINLSSKGVSKEIQAIFHNYLERQNQILRLTTFCDWGLADPNDKTRT
LFSYGFVFAYFLMIGEIAGQLIGINRNAFASDSDVITISSFGIDNSIPFLSSSIGVSSACPQYKPFDYYLVNYFYYLDGN
KFSTSRQHMIGVKDIITCKELSSDLVRLFLAAIDVRHQTGDFLTDEFIYYYNQTIDWLEELVITGIQNIAPPTVCDDFFK
DELNELLKTQECALQPENYLPHLAVNAINHWIIQGKQLNQDSKHYFWWLKGLSLVIYPFMPNLGQTIWGSLGYENSPRIK
DFFALPLHSIQKNVPINRNRISQELMFIKEEHDDEIIA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=392, Percent_Identity=24.234693877551, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788432, Length=204, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=400, Percent_Identity=23.5, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321704, Length=546, Percent_Identity=24.1758241758242, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655311, Length=528, Percent_Identity=22.1590909090909, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 60013; Mature: 59882

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSKKYLLIPSIPTPNGRLHLGHIGGPFLSADILARYLRTRGHSAWVISGTDSFESYAA
CCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHC
GKAEEEHRSPEETCNYYHPLIEEDLRLMGIQMDLFINPLKAPWKYLYQHWHHNIYNQLIN
CCCHHHHCCHHHHHHHHCHHHHHHHHHHEEEHHEEECCCHHHHHHHHHHHHHHHHHHHHC
NGTTLVIKEKFPWDEDSQRYLSGYWLKGDCPICRKKTASYFCENCGTHFRPEELIQEDAF
CCCEEEEEECCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
NQKEVENIFLKLPNGINLSSKGVSKEIQAIFHNYLERQNQILRLTTFCDWGLADPNDKTR
CHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHH
TLFSYGFVFAYFLMIGEIAGQLIGINRNAFASDSDVITISSFGIDNSIPFLSSSIGVSSA
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCC
CPQYKPFDYYLVNYFYYLDGNKFSTSRQHMIGVKDIITCKELSSDLVRLFLAAIDVRHQT
CCCCCCCCEEEEEEEEEECCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDFLTDEFIYYYNQTIDWLEELVITGIQNIAPPTVCDDFFKDELNELLKTQECALQPENY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPHLAVNAINHWIIQGKQLNQDSKHYFWWLKGLSLVIYPFMPNLGQTIWGSLGYENSPRI
CCHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCEEEEECCCCCCHHHHHCCCCCCCCCC
KDFFALPLHSIQKNVPINRNRISQELMFIKEEHDDEIIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNSKKYLLIPSIPTPNGRLHLGHIGGPFLSADILARYLRTRGHSAWVISGTDSFESYAA
CCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHC
GKAEEEHRSPEETCNYYHPLIEEDLRLMGIQMDLFINPLKAPWKYLYQHWHHNIYNQLIN
CCCHHHHCCHHHHHHHHCHHHHHHHHHHEEEHHEEECCCHHHHHHHHHHHHHHHHHHHHC
NGTTLVIKEKFPWDEDSQRYLSGYWLKGDCPICRKKTASYFCENCGTHFRPEELIQEDAF
CCCEEEEEECCCCCCCCHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
NQKEVENIFLKLPNGINLSSKGVSKEIQAIFHNYLERQNQILRLTTFCDWGLADPNDKTR
CHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHH
TLFSYGFVFAYFLMIGEIAGQLIGINRNAFASDSDVITISSFGIDNSIPFLSSSIGVSSA
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCCCC
CPQYKPFDYYLVNYFYYLDGNKFSTSRQHMIGVKDIITCKELSSDLVRLFLAAIDVRHQT
CCCCCCCCEEEEEEEEEECCCEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDFLTDEFIYYYNQTIDWLEELVITGIQNIAPPTVCDDFFKDELNELLKTQECALQPENY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPHLAVNAINHWIIQGKQLNQDSKHYFWWLKGLSLVIYPFMPNLGQTIWGSLGYENSPRI
CCHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCEEEEECCCCCCHHHHHCCCCCCCCCC
KDFFALPLHSIQKNVPINRNRISQELMFIKEEHDDEIIA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA