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The map label for this gene is trmJ [H]

Identifier: 54294634

GI number: 54294634

Start: 1908299

End: 1909072

Strand: Reverse

Name: trmJ [H]

Synonym: lpl1711

Alternate gene names: 54294634

Gene position: 1909072-1908299 (Counterclockwise)

Preceding gene: 54294636

Following gene: 54294633

Centisome position: 57.06

GC content: 38.76

Gene sequence:

>774_bases
ATGAAGTTAAGTTCTATTCGAATTGTTTTAGTTTCAACTTCCCATCCTGGCAATATAGGCTCTACTGCAAGAGCCATGAA
AACCATGGGGCTAAGTACTCTGTACCTGGTTAATCCAAAGTCATTTCCTGATCTCAAAGCAAGAGAAATGGCTGCTGGAG
CCGATGATGTTCTGGATGCAGCAGTTGTTACAAATACCCTGGACGAAGCTTTAATTGGATGTCAGTTGATTTTAGGGACG
AGTGCCAGACCCCGTGGACTGTCATTACCTGGATTAATTCCAGCTTCCTGTGCAGAATTGATAAATCAACAATCAGATAA
TACTCAAGTGGCCATTGTATTTGGTAGAGAACATGCGGGCCTTACTAATGAAGAGCTGTTAAAATGTCACTACCATATCA
ATATTCCAAGCAATCCTGAGTACAGTTCATTAAATTTGGCTCAGGCAGTACAAATTATCGCTTATGAACTTCGTATGAAG
TTATTATCACCGAGTGCTCAAGTAGCTCTGCGCAATGAAGAACCTGCTACAGCTGATGAAATAGAGCAATTTTATGAGCA
TCTGAAAGAAGTTTTTATTGAAATTAATTTTTTGAAGCCCTCTAATCCAAGGAGATTAATGCAAAGAGTGAGGCGATTGT
TTAATAGAATCAACCTTGAAAAAATGGAAGTTAGTATTTTAAGAGGTATGCTTAGCCAGGTTCAGAAATCATTGGAGTGG
GCAAGGAAAGACAATAGGAGCGATAGAATTGGCAATAAAATCGATTTATTTTGA

Upstream 100 bases:

>100_bases
CTATAAAATCAGCAATTCTAACACATTTTTTTTAAACAAAGAGAAGTTCAATTAATAAATCCTATGATATAGTTATCTAT
TGTATTTGGAATAATTTTTT

Downstream 100 bases:

>100_bases
TTATATGGCAACAACACCGGTTGATCCTCGCGTTGTTGAGCAAATGATAAAATATTTAGGCCCTGAAGGGGATTTTGGTA
ATCCTGCATCAGCAACTCAT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAREMAAGADDVLDAAVVTNTLDEALIGCQLILGT
SARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
ARKDNRSDRIGNKIDLF

Sequences:

>Translated_257_residues
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAREMAAGADDVLDAAVVTNTLDEALIGCQLILGT
SARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
ARKDNRSDRIGNKIDLF
>Mature_257_residues
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAREMAAGADDVLDAAVVTNTLDEALIGCQLILGT
SARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
ARKDNRSDRIGNKIDLF

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=249, Percent_Identity=51.004016064257, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1790865, Length=234, Percent_Identity=30.7692307692308, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28681; Mature: 28681

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAREMAAGADDVLDA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCHHHHHHH
AVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAG
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
LTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMKLLSPSAQVALRNEEPATADE
CCHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
IEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARKDNRSDRIGNKIDLF
HHCCCCCHHCCCCCCCC
>Mature Secondary Structure
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAREMAAGADDVLDA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCHHHHHHH
AVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAG
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
LTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMKLLSPSAQVALRNEEPATADE
CCHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
IEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARKDNRSDRIGNKIDLF
HHCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]