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The map label for this gene is purN [H]

Identifier: 54294561

GI number: 54294561

Start: 1823707

End: 1824285

Strand: Reverse

Name: purN [H]

Synonym: lpl1637

Alternate gene names: 54294561

Gene position: 1824285-1823707 (Counterclockwise)

Preceding gene: 54294562

Following gene: 54294558

Centisome position: 54.53

GC content: 38.51

Gene sequence:

>579_bases
ATGATTCGTTTAGGAATATTAGGGTCTACTCGTGGAACCAATATGTTGGCTCTTGTTGATGCAATCAATGAGGGAATTCT
TAAGGCAAAGATAGAGCTTGTTATTAGTAATAAATCTGATGCCATTATTCTGGAAAGAGCCAAATCGTTGGGTTTGAATG
CACAGTTTGTCAATCCTGAGGGATTAAACAGAATTGATTTTGATAAAAAAGTAAGTGACATACTGATCAACCATCAGATT
GATTTGATTGTTTTGATCGGGTACATGAGAATTTTGTCGGCGGATTTCGTAAATAAATGGAATAATCAGGTAATTAATGT
ACATCCCTCTTTATTGCCAGCATTTGCAGGGAAAATGGATATGGATGTACATCAAGCCGTTCTTGATTCCGGCCTAAAGG
AAACAGGTTGTACTATCCATTTTGTCACTGAAGAGGTGGATGCTGGCCCTGTTATTTTGCAAAAAAAATGCCCTGTCTTA
GAAGGTGATACAGCTCAAACGTTAAAAGCAAGGGTGCAGCAGTTGGAAGGAATGGCTTTGGTAGCTGCAATTAACCTGAT
TGCATCGAAAGGTCTCTAA

Upstream 100 bases:

>100_bases
AGATAAAAAATATAAAAGGCCCTTTATTTCACAGAAAAGATATTGGCACATCGCCTTTAATTAATACACGCGTTGAAGTA
ATGCGTAGGTTGCGTGCCTT

Downstream 100 bases:

>100_bases
TACTGGATTGTATTTCCAGACAGAGCAGTATGGTCTTCATTTCCATGAGAGTAGTTTTTTTCTTTTTCAATTGTAAAAAA
AGTGTTTAACAAAGTTTCTT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIRLGILGSTRGTNMLALVDAINEGILKAKIELVISNKSDAIILERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQI
DLIVLIGYMRILSADFVNKWNNQVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
EGDTAQTLKARVQQLEGMALVAAINLIASKGL

Sequences:

>Translated_192_residues
MIRLGILGSTRGTNMLALVDAINEGILKAKIELVISNKSDAIILERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQI
DLIVLIGYMRILSADFVNKWNNQVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
EGDTAQTLKARVQQLEGMALVAAINLIASKGL
>Mature_192_residues
MIRLGILGSTRGTNMLALVDAINEGILKAKIELVISNKSDAIILERAKSLGLNAQFVNPEGLNRIDFDKKVSDILINHQI
DLIVLIGYMRILSADFVNKWNNQVINVHPSLLPAFAGKMDMDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVL
EGDTAQTLKARVQQLEGMALVAAINLIASKGL

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=43.0851063829787, Blast_Score=160, Evalue=8e-40,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=43.0851063829787, Blast_Score=160, Evalue=8e-40,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=43.0851063829787, Blast_Score=160, Evalue=8e-40,
Organism=Escherichia coli, GI1788846, Length=172, Percent_Identity=38.953488372093, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787483, Length=162, Percent_Identity=34.5679012345679, Blast_Score=91, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=36.5079365079365, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320616, Length=204, Percent_Identity=27.4509803921569, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=36.7021276595745, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20849; Mature: 20849

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLGILGSTRGTNMLALVDAINEGILKAKIELVISNKSDAIILERAKSLGLNAQFVNPE
CEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEHHHHCCCCEEEECCC
GLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNNQVINVHPSLLPAFAGKMD
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCC
MDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVLEGDTAQTLKARVQQLEGMAL
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHH
VAAINLIASKGL
HHHHHHHHHCCC
>Mature Secondary Structure
MIRLGILGSTRGTNMLALVDAINEGILKAKIELVISNKSDAIILERAKSLGLNAQFVNPE
CEEEEEECCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEHHHHCCCCEEEECCC
GLNRIDFDKKVSDILINHQIDLIVLIGYMRILSADFVNKWNNQVINVHPSLLPAFAGKMD
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCC
MDVHQAVLDSGLKETGCTIHFVTEEVDAGPVILQKKCPVLEGDTAQTLKARVQQLEGMAL
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHH
VAAINLIASKGL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]