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The map label for this gene is empA [H]

Identifier: 54294544

GI number: 54294544

Start: 1794441

End: 1796114

Strand: Reverse

Name: empA [H]

Synonym: lpl1620

Alternate gene names: 54294544

Gene position: 1796114-1794441 (Counterclockwise)

Preceding gene: 54294545

Following gene: 54294543

Centisome position: 53.68

GC content: 36.62

Gene sequence:

>1674_bases
ATGAACACACGGATAGTTTCATTATTAGTTGTTTTTATGGCAAGTGCTAATTTGCAAGCTGCTTCAGAAAGTATTATTTG
GCAGGCGGGCGATAACAATTTAAAAAAATACAATGTTAGTATCTTGCAAGATAAAAAAGGATTTATATCAATTACTAATA
AAATCACGCCGTTAAATTATCAGCTGAAACCAGTTGATCATGAGGGCAATCGTGATTATAACGATATTCGTTACCAGGTT
TATTATAGAAATATTCCTGTTTGGGGTCATGAGCTAATCCTTCATAAGACCAGCAAGTCAAAAATTTTTCTGACCGGTGT
CGATGTGTCTGAAATTGAAAAGGATGTTCATCAGACTGATGGGAAATTAAGTGCTGCTGATATTGAAAGCCAGATAATAA
AATATAATAACAATTCGATCGTTTACAAAAATATAAAAAAAATCATTTATCTGGATTCACATAAAAAAGCTTATCTAGCT
TATCATTTATCAATGTATGCAAATGATCCATTACATTTTGTCTCCGCTCCGAATTATATTATCGATGCCAATTCTGGTGA
GATTTTAAAAAGCTGGGATGATTTAACACATAAGAAAGTTGGTCAGGGTTTGGGTGGTAATGTATTCATTCTTCCCTATC
GGTCGGGATTGTTTCAACATGGTGATGCAATGAAGGATATTCCTTCTCTCGGCAAATTTGAGGTAACAATCAAGGGGAAA
AATTGTGTTGTAGAAAGCCCTGAAGTAAGGGTCATTAATGCCGCCTATACTGATTTGGACAAGAGATCCTTCCCCGTTCT
AAGTATTGTGGAAATCTATAAAAATATACCAGCATTTTTCTACCCCTGTAGCAAAGAATCACAATATATCAATCTTAATG
ATGGAGATACATCACCTGCAAACTACAGTTTTTCTTCAATTAATGATGCTATGTATTTTGCCGGAGTCACACTGGAAATG
TACAAAGATTATTATGGTGTTGAAAAACCTTTAGGCGATGACTTGCCTTTGCGAGCCTATACACATATTAAAAATTTTGA
TAATGCCTTTGCTGTGCCTTCTATTAAAATAAAAGGATTGTACTTAATGCATCAACAAATTGTGATTGGCGATGGGGATA
CTCAATTAACAGCACCGGCACAGAGCACTTTATCGCATGAGTTGTCTCATAATTTTACCAGATTGCATTCTAACCTGGTC
TATAATGGGCAATCCGGAGGAATCAATGAAGCATTCTCGGATATGGCATCGATTGCCATGCAGGATTACTTGCGTAAAGA
TTTTCCATGGTATTGGGATGGTGAAGATTGGTCTATAGGGCGTGAGGCTACTATAAGTGGTGAGCCAATTCGTTATATGG
ACTATCCAACGAAAGATGGTCATTCTATTGAGCATTTCGATGATTATGATGATGACTTGGATGTTCATCTGACCAGCGGG
ATCTTTAACAGAGCTTTTTATCTTTTAGCACATCAACCGAACTGGTCAGTGAGAAAGGCATTTCAAGTGATGGTAGATGC
CAATATAAAATATTGGACGTCAGGGACTTCATTTGAAGCAGCCTCTTGTGGTGTTATCCAAGCGGCAATTGACAGGCAAT
ATAATAAGCAGGATGTCATCAATGCTTTTAAAGAGGTAGGAGTGATTTGTCCTGTTATTGCTTTGGTCAATTAA

Upstream 100 bases:

>100_bases
CGTCTGCTATGGCTGATGCAGTATGCAAACACATATCAGGAGTCAGTGAATATAAATAAACGCTCTAAGACACAACAATA
GTCAAAGACAGGGAAAAAAT

Downstream 100 bases:

>100_bases
CCACTGTCATTCGACTAATAAATTCCCTTCATTAATGGAGGGGATTCTACCCAGCAACAGCATATCACCTGTGAAGAGGT
ATTCTTACTCTATGTTTGAT

Product: hypothetical protein

Products: NA

Alternate protein names: Vibriolysin [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNTRIVSLLVVFMASANLQAASESIIWQAGDNNLKKYNVSILQDKKGFISITNKITPLNYQLKPVDHEGNRDYNDIRYQV
YYRNIPVWGHELILHKTSKSKIFLTGVDVSEIEKDVHQTDGKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLA
YHLSMYANDPLHFVSAPNYIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLFQHGDAMKDIPSLGKFEVTIKGK
NCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNIPAFFYPCSKESQYINLNDGDTSPANYSFSSINDAMYFAGVTLEM
YKDYYGVEKPLGDDLPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNFTRLHSNLV
YNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPIRYMDYPTKDGHSIEHFDDYDDDLDVHLTSG
IFNRAFYLLAHQPNWSVRKAFQVMVDANIKYWTSGTSFEAASCGVIQAAIDRQYNKQDVINAFKEVGVICPVIALVN

Sequences:

>Translated_557_residues
MNTRIVSLLVVFMASANLQAASESIIWQAGDNNLKKYNVSILQDKKGFISITNKITPLNYQLKPVDHEGNRDYNDIRYQV
YYRNIPVWGHELILHKTSKSKIFLTGVDVSEIEKDVHQTDGKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLA
YHLSMYANDPLHFVSAPNYIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLFQHGDAMKDIPSLGKFEVTIKGK
NCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNIPAFFYPCSKESQYINLNDGDTSPANYSFSSINDAMYFAGVTLEM
YKDYYGVEKPLGDDLPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNFTRLHSNLV
YNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPIRYMDYPTKDGHSIEHFDDYDDDLDVHLTSG
IFNRAFYLLAHQPNWSVRKAFQVMVDANIKYWTSGTSFEAASCGVIQAAIDRQYNKQDVINAFKEVGVICPVIALVN
>Mature_557_residues
MNTRIVSLLVVFMASANLQAASESIIWQAGDNNLKKYNVSILQDKKGFISITNKITPLNYQLKPVDHEGNRDYNDIRYQV
YYRNIPVWGHELILHKTSKSKIFLTGVDVSEIEKDVHQTDGKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLA
YHLSMYANDPLHFVSAPNYIIDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLFQHGDAMKDIPSLGKFEVTIKGK
NCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNIPAFFYPCSKESQYINLNDGDTSPANYSFSSINDAMYFAGVTLEM
YKDYYGVEKPLGDDLPLRAYTHIKNFDNAFAVPSIKIKGLYLMHQQIVIGDGDTQLTAPAQSTLSHELSHNFTRLHSNLV
YNGQSGGINEAFSDMASIAMQDYLRKDFPWYWDGEDWSIGREATISGEPIRYMDYPTKDGHSIEHFDDYDDDLDVHLTSG
IFNRAFYLLAHQPNWSVRKAFQVMVDANIKYWTSGTSFEAASCGVIQAAIDRQYNKQDVINAFKEVGVICPVIALVN

Specific function: Extracellular zinc metalloprotease involved in the virulence mechanism of V.anguillarum [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR007280
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C; PF04151 PPC [H]

EC number: NA

Molecular weight: Translated: 63012; Mature: 63012

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRIVSLLVVFMASANLQAASESIIWQAGDNNLKKYNVSILQDKKGFISITNKITPLNY
CCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEECCCCCCCE
QLKPVDHEGNRDYNDIRYQVYYRNIPVWGHELILHKTSKSKIFLTGVDVSEIEKDVHQTD
EEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHCC
GKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLAYHLSMYANDPLHFVSAPNYI
CCEEHHHHHCEEEEECCCEEEEEECEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCEE
IDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLFQHGDAMKDIPSLGKFEVTIKGK
EECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHCCCHHHHCCCCCEEEEEEECC
NCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNIPAFFYPCSKESQYINLNDGDTSPA
EEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCC
NYSFSSINDAMYFAGVTLEMYKDYYGVEKPLGDDLPLRAYTHIKNFDNAFAVPSIKIKGL
CCCHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEE
YLMHQQIVIGDGDTQLTAPAQSTLSHELSHNFTRLHSNLVYNGQSGGINEAFSDMASIAM
EEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
QDYLRKDFPWYWDGEDWSIGREATISGEPIRYMDYPTKDGHSIEHFDDYDDDLDVHLTSG
HHHHHHCCCEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECC
IFNRAFYLLAHQPNWSVRKAFQVMVDANIKYWTSGTSFEAASCGVIQAAIDRQYNKQDVI
HHCCEEEEEEECCCCHHHHHEEEEEECCEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHH
NAFKEVGVICPVIALVN
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTRIVSLLVVFMASANLQAASESIIWQAGDNNLKKYNVSILQDKKGFISITNKITPLNY
CCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCCEEEEECCCCCCCE
QLKPVDHEGNRDYNDIRYQVYYRNIPVWGHELILHKTSKSKIFLTGVDVSEIEKDVHQTD
EEEECCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHCC
GKLSAADIESQIIKYNNNSIVYKNIKKIIYLDSHKKAYLAYHLSMYANDPLHFVSAPNYI
CCEEHHHHHCEEEEECCCEEEEEECEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCEE
IDANSGEILKSWDDLTHKKVGQGLGGNVFILPYRSGLFQHGDAMKDIPSLGKFEVTIKGK
EECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHCCCHHHHCCCCCEEEEEEECC
NCVVESPEVRVINAAYTDLDKRSFPVLSIVEIYKNIPAFFYPCSKESQYINLNDGDTSPA
EEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCEEECCCCCCCCC
NYSFSSINDAMYFAGVTLEMYKDYYGVEKPLGDDLPLRAYTHIKNFDNAFAVPSIKIKGL
CCCHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEE
YLMHQQIVIGDGDTQLTAPAQSTLSHELSHNFTRLHSNLVYNGQSGGINEAFSDMASIAM
EEEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
QDYLRKDFPWYWDGEDWSIGREATISGEPIRYMDYPTKDGHSIEHFDDYDDDLDVHLTSG
HHHHHHCCCEEECCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEECC
IFNRAFYLLAHQPNWSVRKAFQVMVDANIKYWTSGTSFEAASCGVIQAAIDRQYNKQDVI
HHCCEEEEEEECCCCHHHHHEEEEEECCEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHH
NAFKEVGVICPVIALVN
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429449; 2228244 [H]