| Definition | Legionella pneumophila str. Lens, complete genome. |
|---|---|
| Accession | NC_006369 |
| Length | 3,345,687 |
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The map label for this gene is iolD [H]
Identifier: 54294540
GI number: 54294540
Start: 1787953
End: 1789824
Strand: Reverse
Name: iolD [H]
Synonym: lpl1616
Alternate gene names: 54294540
Gene position: 1789824-1787953 (Counterclockwise)
Preceding gene: 54294541
Following gene: 54294539
Centisome position: 53.5
GC content: 40.38
Gene sequence:
>1872_bases ATGAAGATTAAATTAACTATGGCTCAGGCCCTATTAAGATTTTTAGCTAATCAGTATGTCGTTCTGGATAATAATGAATA CCGTTTCATCAATGGTGTGTTTGGAATATTTGGCCATGGTAATGTAACCGGATTAGGTGAGGCATTGGAATACGATGATC AGGGTTTAATTTATTATCAGGGACATAATGAACAAGGAATGGCTCACGCAGCAACCGCCTATGCCAAACAAAAAAATCGG CTAGGGATAATGGCTTGTACTTCATCAATTGGTCCTGGCGCTACTAATATGATTACTGCAGCGGCGACAGCTACAACCAA TAGAATCCCATTACTGTTGTTGCCAGGTGATGTATTCAGTTGCAGGCAACCAGATCCGGTTCTCCAACAATTGGAGGTGC CATATGACTACACCATATCTGTTAATGATTGCTTTAAACCAGTAAGTAAGTTTTGGGACAGGATAACCAGACCTGAACAG TTAATGACAGCATGTATGCATGCTATGAGGGTATTAACCGATCCAGTTGAAACAGGTACTGTTACTTTGTGTTTACCTCA AGACGTTCAGTCTGAAGCTTATGAATATGAGGAATCTTTTTTTAAAAAGCGCTTATGGCGTATTGAGAGAGAAAGGATAA GTGATGCAATGTTGGCAGAGGCCATACGGCATATCAGAAAGGCCAAGCAACCATTAATCATTGCAGGTGGTGGTGTGCAT TATTCACTGGCTTCGGAGACTTTATCAGAGTTTGCATTAAAACATCGCATTCCTGTTGCTGAAACCCAAGCAGGAAAAAG TAGCCTGGCAGCGAAACACCCCATGAACGTTGGTGGAATAGGTGTTACTGGATCTGAAATAGCGAATTCTCTGGCAGCTC AGGCTGATGTTATTTTGGTTATCGGGTCTCGATTACAGGATTTTACGACAGCTTCCAAATGGGCTTTTAAAAACCAGGAC TGCCAAATCATTCATCTTAATGTATCACGTCTTGATGCCATGAAAATGAATGGAGTTATGTTAAAAGGAGATGCTAAAAC TGGTTTGGAGCAATTGGGGAAAGAGCTGGCAGATTATCAAACCTCTCTCTCTTACCAGCAGTTAATATGTCATTATCAAA ATGAATGGGCTCAAGAGTTAGATAAATTGACTCAGTCTTATTCTACAGAGCAGGCTGGGTTACACCAAACTACTGTTTTG GGACTGCTCAACCAGTATGTTACTGAAGAGGATGTGATAATTAGTGCAGCGGGGAGTTTGCCAGGTGATTTGCATCGAAT TTGGCAATCCAAAAAGGCAAAAGATTATCATTTGGAGTATGCTTATTCCTGTATGGGATATGAGGTTGCAGCTGGCCTAG GAGTACGTATTGCAAAAGAAAATCAGGAAGGAGAGGTCTATATTTTAGTTGGAGATGGCAGTTTTGTCATGATGCATTCA GAGCTTTTAACTGCCATCCAGGAACGCAAGAAAATTACAATTCTCATTTTTGATAACCATGGATTTCAGTGCATTAGAAA TTTACAGGAAGGGAGTGGTAGTCTTGGTTTTGGTAATGAATTTCGTTATAGGGAGCCAACTACTAATGCTTTAAATGGCG ATTATTTACCCATAGATTTTTGTAAGTACGCAGAGGGATTGGGGGCAGCTACTTATTTTGTTAAATCCTATGAGCAATTC CGGACTACTTTAGACTCAGCAAAAAAACAGGAAAATAGTGCAGTTATTGTTCTCCCTGTATTACCAAAGACCATGAGTCA AGGATATAAGACTTGGTGGCGGGTTGGAGTTGCTGAGGTATCTCAATCAGAATCAGTGAGTAAAGCTTACCAAATGATGC GAGAGCAAATTGAAACAGTTAGACAATATTGA
Upstream 100 bases:
>100_bases AAGACCATGAATGGACAAGGACAAGCAGGGCAAATTTAAGAGCATGGCAACAGAAACAGTAACAATCCTTATCTGCAGTA TGATTTTGAGAGTTAAATAA
Downstream 100 bases:
>100_bases AGATGGAAGTGAAGATGAGTATTCAACTTGGTATCGCACCAATTAATTGGTGCAATGATGATGATCCGAATTTAGGTAAA GACATTAGTTTTGAGCAATG
Product: hypothetical protein
Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]
Alternate protein names: THcHDO hydrolase 1 [H]
Number of amino acids: Translated: 623; Mature: 623
Protein sequence:
>623_residues MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDDQGLIYYQGHNEQGMAHAATAYAKQKNR LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD CQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLICHYQNEWAQELDKLTQSYSTEQAGLHQTTVL GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYILVGDGSFVMMHS ELLTAIQERKKITILIFDNHGFQCIRNLQEGSGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQF RTTLDSAKKQENSAVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY
Sequences:
>Translated_623_residues MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDDQGLIYYQGHNEQGMAHAATAYAKQKNR LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD CQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLICHYQNEWAQELDKLTQSYSTEQAGLHQTTVL GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYILVGDGSFVMMHS ELLTAIQERKKITILIFDNHGFQCIRNLQEGSGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQF RTTLDSAKKQENSAVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY >Mature_623_residues MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDDQGLIYYQGHNEQGMAHAATAYAKQKNR LGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFSCRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQ LMTACMHAMRVLTDPVETGTVTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILVIGSRLQDFTTASKWAFKNQD CQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQTSLSYQQLICHYQNEWAQELDKLTQSYSTEQAGLHQTTVL GLLNQYVTEEDVIISAAGSLPGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYILVGDGSFVMMHS ELLTAIQERKKITILIFDNHGFQCIRNLQEGSGSLGFGNEFRYREPTTNALNGDYLPIDFCKYAEGLGAATYFVKSYEQF RTTLDSAKKQENSAVIVLPVLPKTMSQGYKTWWRVGVAEVSQSESVSKAYQMMREQIETVRQY
Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]
COG id: COG3962
COG function: function code E; Acetolactate synthase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family [H]
Homologues:
Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=23.2682060390764, Blast_Score=82, Evalue=2e-15, Organism=Escherichia coli, GI1790104, Length=510, Percent_Identity=24.7058823529412, Blast_Score=133, Evalue=3e-32, Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=23.4347048300537, Blast_Score=103, Evalue=2e-23, Organism=Escherichia coli, GI1788716, Length=509, Percent_Identity=25.7367387033399, Blast_Score=99, Evalue=9e-22, Organism=Escherichia coli, GI1786717, Length=527, Percent_Identity=21.8216318785579, Blast_Score=94, Evalue=2e-20, Organism=Escherichia coli, GI1787096, Length=445, Percent_Identity=20, Blast_Score=69, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17531299, Length=511, Percent_Identity=21.9178082191781, Blast_Score=70, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17531301, Length=511, Percent_Identity=21.9178082191781, Blast_Score=70, Evalue=3e-12, Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=24.6478873239437, Blast_Score=122, Evalue=2e-28, Organism=Drosophila melanogaster, GI19922626, Length=594, Percent_Identity=21.8855218855219, Blast_Score=85, Evalue=1e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012000 - InterPro: IPR012001 - InterPro: IPR000399 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]
EC number: 2.2.1.6 [C]
Molecular weight: Translated: 69496; Mature: 69496
Theoretical pI: Translated: 6.46; Mature: 6.46
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDDQGLIYYQ CEEEEHHHHHHHHHHCCCEEEEECCCEEEEECEEEECCCCCCCCCCHHHCCCCCCEEEEE GHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFS CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHCCCCCCEEEEECCCCCC CRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQLMTACMHAMRVLTDPVETGT CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE VTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILV EHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEE IGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQ ECCHHHHHHHHHHCEECCCCCEEEEEEHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH TSLSYQQLICHYQNEWAQELDKLTQSYSTEQAGLHQTTVLGLLNQYVTEEDVIISAAGSL HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCC PGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYILVGDGSFVMMHS CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCEEEEEEECCCEEEEHH ELLTAIQERKKITILIFDNHGFQCIRNLQEGSGSLGFGNEFRYREPTTNALNGDYLPIDF HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH CKYAEGLGAATYFVKSYEQFRTTLDSAKKQENSAVIVLPVLPKTMSQGYKTWWRVGVAEV HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH SQSESVSKAYQMMREQIETVRQY HHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MKIKLTMAQALLRFLANQYVVLDNNEYRFINGVFGIFGHGNVTGLGEALEYDDQGLIYYQ CEEEEHHHHHHHHHHCCCEEEEECCCEEEEECEEEECCCCCCCCCCHHHCCCCCCEEEEE GHNEQGMAHAATAYAKQKNRLGIMACTSSIGPGATNMITAAATATTNRIPLLLLPGDVFS CCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEEEHHCCCCCCEEEEECCCCCC CRQPDPVLQQLEVPYDYTISVNDCFKPVSKFWDRITRPEQLMTACMHAMRVLTDPVETGT CCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCE VTLCLPQDVQSEAYEYEESFFKKRLWRIERERISDAMLAEAIRHIRKAKQPLIIAGGGVH EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEE YSLASETLSEFALKHRIPVAETQAGKSSLAAKHPMNVGGIGVTGSEIANSLAAQADVILV EHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEE IGSRLQDFTTASKWAFKNQDCQIIHLNVSRLDAMKMNGVMLKGDAKTGLEQLGKELADYQ ECCHHHHHHHHHHCEECCCCCEEEEEEHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH TSLSYQQLICHYQNEWAQELDKLTQSYSTEQAGLHQTTVLGLLNQYVTEEDVIISAAGSL HCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCC PGDLHRIWQSKKAKDYHLEYAYSCMGYEVAAGLGVRIAKENQEGEVYILVGDGSFVMMHS CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCEEEEECCCCCEEEEEEECCCEEEEHH ELLTAIQERKKITILIFDNHGFQCIRNLQEGSGSLGFGNEFRYREPTTNALNGDYLPIDF HHHHHHHHCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH CKYAEGLGAATYFVKSYEQFRTTLDSAKKQENSAVIVLPVLPKTMSQGYKTWWRVGVAEV HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH SQSESVSKAYQMMREQIETVRQY HHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: FAD [C]
Metal ions: Mg2+ [C]
Kcat value (1/min): 4002 [C]
Specific activity: 53
Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]
Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]
Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]
General reaction: C-C bond formation; decarboxylation [C]
Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA