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The map label for this gene is rumA [H]

Identifier: 54294503

GI number: 54294503

Start: 1752838

End: 1754172

Strand: Reverse

Name: rumA [H]

Synonym: lpl1572

Alternate gene names: 54294503

Gene position: 1754172-1752838 (Counterclockwise)

Preceding gene: 54294505

Following gene: 54294502

Centisome position: 52.43

GC content: 37.9

Gene sequence:

>1335_bases
ATGAGAAAAGTTAAGCCAAAATTGAATTTAACCTCACAAACCGCTCGGATCGTAAATTTAAGCCATGATGGAAGGGGGAT
AGCACGAATTAATGGCAAAGCAACTTTTATTCAGGGAGCATTACCCGGTGAGGTGGTTGAATTTCAGTATACACGGGTAA
AAAAGGATTTTGATGAAGGTAAATTATTATCAATTGTCGAGCCTTCGACTCTCAGGGTTGAACCAAAATGCCCTCATTAT
CAAATGTGTGGAGGCTGTTCTTTGCAACATATGAGTGCCGAGGAACAAATTCGTTTCAAACAATCCCATCTTTTGGATTT
ACTATCTCGGTATGGCCATACTGCACCTCAAACAGTATTGTCACCTTTAACAAGCCATCCTTGGAATTATAGAAATAAAG
CACGCTTAAGTACTCGCTTTGTAGAAAAGAAACAAAGCACCATGGTTGGATTTAGAGAGAGGAATAATCCAAGGTTCATT
ACAGAAATAAATCAATGTCCTATTCTTAATTCAAAAATAGATACTGATATTGTCCATTTGAGAAAATTAATCGATACGAT
GGAAGATAAGCAGTGTATCGCACAAATTGAAGTGGCTGCAGGTGATAATGAAGTGGCGCTGATTTTCCGCAATCTAACCC
CATTAACAGAGCAAGATGAGTCGAAAATCAGGAAATTTGCGCAACAATTCCAATACAAGGTATTCCTGCAGCCAGGTGGA
CTTGATTCGGTTTTTTGTTTTTATCCTTCAGATGCTCATGCTTATTTGAGTTATGAGCTGCCCGATTATCAAATAACTTT
TCAATTTCATCCCAACGATTTTACTCAGGTAAACGCCGAGTTAAATAGAAAGATGGTAAAGCAGGCAATTCAGTTAATGG
AATTAAAAAATTCTGATATCGTGCTAGACTTATTTTGTGGACTGGGTAATTTTTCGCTTCCCATGGCTAAACATTGCTCT
CGAGTTATCGGTGTCGAAGGCAATAGGAACATGGTTGAAAGAGCCTATATGAATGCCAAGTCTAATCATATTACCAATGT
GGATTTTTATGCTGCTAATCTTGATGATGTGATGGAAGTTAGGAATCTGGTTAATACGTCTTTTAGCAAGGTTTTAATCG
ATCCTCCCCGGTCTGGTGCGCTTGAAATTGTAAAACAAATTGATTCAATTGATCCCGAGCGTATAGTATATGTATCTTGT
AACCCGATAACATTAGCTCGAGATACAGATATATTGGTTAATCAAAAAGGTTACGTTTTGATAACAGCTGGGGTAATGGA
TATGTTTCCTCATACAGCGCATGTCGAGTCTATTGCCTTGTTTCAAAAAGGATAA

Upstream 100 bases:

>100_bases
CGGCCCTTTATTTGGGCATTTTTTGTTTTTTATATCCATACAACATTCTAAAAATGTAGCATGAGAATTTTGTAATTGAT
AATTATTTGAGAATCATTTC

Downstream 100 bases:

>100_bases
TATGTATGGTAAGAGTAAAAGATACGACTCCGTTGACGGCAGATGGCAGTATTGACGTAGAGATGTGGTTACATCATCTT
GGTTCAAAAGGATATTTGGA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARINGKATFIQGALPGEVVEFQYTRVKKDFDEGKLLSIVEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTAPQTVLSPLTSHPWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDESKIRKFAQQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVKQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNRNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG

Sequences:

>Translated_444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARINGKATFIQGALPGEVVEFQYTRVKKDFDEGKLLSIVEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTAPQTVLSPLTSHPWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDESKIRKFAQQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVKQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNRNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG
>Mature_444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARINGKATFIQGALPGEVVEFQYTRVKKDFDEGKLLSIVEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTAPQTVLSPLTSHPWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDESKIRKFAQQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVKQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNRNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=193, Percent_Identity=31.6062176165803, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI51173878, Length=193, Percent_Identity=31.6062176165803, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI269784678, Length=249, Percent_Identity=26.1044176706827, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI269784680, Length=249, Percent_Identity=26.1044176706827, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI269784676, Length=249, Percent_Identity=26.1044176706827, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI20149688, Length=249, Percent_Identity=26.1044176706827, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789148, Length=433, Percent_Identity=36.2586605080831, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1790403, Length=177, Percent_Identity=31.638418079096, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787083, Length=180, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666579, Length=200, Percent_Identity=32, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50447; Mature: 50447

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVKPKLNLTSQTARIVNLSHDGRGIARINGKATFIQGALPGEVVEFQYTRVKKDFDEG
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCCCCEEEEEHHHHHCCCCCC
KLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTAPQTVL
CEEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SPLTSHPWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFITEINQCPILNSKIDTDIVHL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
RKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDESKIRKFAQQFQYKVFLQPGG
HHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCC
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVKQAIQLMELKNSDI
CCCEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCCE
VLDLFCGLGNFSLPMAKHCSRVIGVEGNRNMVERAYMNAKSNHITNVDFYAANLDDVMEV
EEEEEECCCCCCCCHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
RNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSCNPITLARDTDILVNQKGYVL
HHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCEEE
ITAGVMDMFPHTAHVESIALFQKG
EEECHHHHCCCHHHHHHEEEEECC
>Mature Secondary Structure
MRKVKPKLNLTSQTARIVNLSHDGRGIARINGKATFIQGALPGEVVEFQYTRVKKDFDEG
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCCCCEEEEEHHHHHCCCCCC
KLLSIVEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTAPQTVL
CEEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SPLTSHPWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFITEINQCPILNSKIDTDIVHL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
RKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDESKIRKFAQQFQYKVFLQPGG
HHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCC
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVKQAIQLMELKNSDI
CCCEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCCE
VLDLFCGLGNFSLPMAKHCSRVIGVEGNRNMVERAYMNAKSNHITNVDFYAANLDDVMEV
EEEEEECCCCCCCCHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
RNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSCNPITLARDTDILVNQKGYVL
HHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCEEE
ITAGVMDMFPHTAHVESIALFQKG
EEECHHHHCCCHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA