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The map label for this gene is mnmC [H]

Identifier: 54294425

GI number: 54294425

Start: 1662447

End: 1664447

Strand: Direct

Name: mnmC [H]

Synonym: lpl1494

Alternate gene names: 54294425

Gene position: 1662447-1664447 (Clockwise)

Preceding gene: 54294424

Following gene: 54294426

Centisome position: 49.69

GC content: 37.68

Gene sequence:

>2001_bases
GTGAGTAGTCCCTTTGTCCCAATTATAACGGCAGATATCGATTGGCGTGATGATCTGCCGTATTCCTTACAATTTGATGA
TATTTACTATTCTGCTGAAGGTGGAATTAATCAAAGTCTTTATGTTTTTGTCGAAGGTAATAATTTGATTAATCGTTGGC
AACAATTACCAACCAATGAATCAAATGTATTTACAATCGCTGAAACTGGTTTCGGTACAGGTATGAATTTCTTATTAACC
TGGAAACTATGGGAAAAATTTGCACCTCAAAATGCCCGCTTACATTATATTTCCTGTGATAAACATCCATTAAAAAAAGA
TGACCTAATCAAATGCTTGCAAAAGTGGCCAGAACTGAGTGTGCAGACTGAAAAATTAATAGAACACTATCCTGTTTTGA
CACCTGGTTATCATCATTTAGCATTTAGTAACAATCGGATTACTTTAACATTAATGCTAGGAGACGTGCTGGAGTGCTAT
GAGCAATTATTATTCTGTGGGGACATTAACCTTGAACATCAATTGAGAGAATCCTATGTTAATGCTTGGTACCTGGATGG
ATTTTCACCATCAAAAAACCAATCCATGTGGTCTGACAATCTGTTCACTGTGATTGCCATGCTATCTAAAGAAAGCACCA
CAGTAGCTACCTATTCTGCTTCCAGTATCGTCAAAACTGCTTTAACCAATGCAGGATTTGTTATTAATAAAAGAAAGGGA
TTTGGGCCCAAACGACACATGATATGCGCCCATTATGAAAAAGCTTATTCAAGTTCAAAAAAAAATCGCCATACCCCGTG
GCACATAAACTATCCTGTGACAAAGGAAGAAAGAACAGCGCTCATTGTTGGAGGAGGGCTTGCAGGTTGTTTTATTGCAA
ATAGCCTCGCTAAGCGTGGATGGGAAGTGACCATATTAGAAGAAAAAGAGAAAGTGGGATGTGGTGGATCGGCAAATCAA
CAGGCTGTATTGTTCCCTAAGTTATCCATCTATAAATCACCATTTACTCAGTTTATGCTTTATTCGTTTCTTTATGCTAA
TGACGTTTACAAGGAACTTTTGAAACATTATAACCTTGGTGAATTAAAAGGCTCTTTGTTGTTAGCGCATAACGAGAGGG
AAAAAGCAAACCAGCAAAGTCTTATTCATTGGCTAGAATTATACCCTGAATTAGGGCAATTAGTGGATGAAAAGCAATCT
TCTGAATTATCAGGGATATCCTTGCCATGTGGTGGTTTATTTATTCCATCTTCCGGCTGGATTAATTCCCCTGAACTATG
CGACATTCTAATTGATAATAAAAGAATTTCTCTAATCACTGGCAATAGAGTTCAATCTATTAACTATAATCAAAAAAACT
GGGTAGTTAATGACATCGACGCTTCGGTACTAATTTTGGCAAATGGACAGCAAGTCAATTATTTTCACGAAACCAATCAT
TTGCCTGTAAAAGCCATTAGAGGACAAATGACTACGATTCAATCAACGCAAGAAAGTACCAAATTGAAGATTCCTTTATG
TGCTGAGGGGCATGTTTTACCAGCTTTAAACAATAGTCATAGAGTGGGGGCAAGTTATGATATTGGCACATCTGAACCTG
AACTTAATGCTCTGGATGATCAATTAAACCTCGACCGATTAAAACGGATAGCGCCTGATATAATCTGGTCCCAAAACGTG
TTAGACCATTGGGCTGGTATACGAGCCGCATCACCAGATTACTTACCTATAGTGGGACCTCTCCCCAATGCGCTTGAATT
TAAAGAAATTTATTCAGAATTAAAATCTAACTCCAAACGATGGATAGCTGAAGCAGCTCCTTGTTATCCAAATCTATATG
TGTGTGCGGCTTTTGGATCAAGAGGATTAACAACAATACCTCTCGCAACTGAATGGTTAGCTGGTTTAATTAATAGGGAA
ATTTCAATACTGCCCAGAAAATTGATACAAGCTATATCGCCCGCACGATTCTTAAGAAAAAAAATTATACAGGGACCATA
A

Upstream 100 bases:

>100_bases
CTTTTCATTTTAGTGCCTAAACTAAGAAAGCTGATCAAAGAAAAAAACTTGAATGGCTTTAAACAGTCTGGCTTAGAGGT
AACTGAAAGAGTAGCTTTAT

Downstream 100 bases:

>100_bases
GAGATATATTGAAGATATATTGATAATTCGACTTAACTTCATCTTTCTTTATCAGTTATAATTTTAATTTTTATGTGAGC
TGATTGTGCAGAATATTGAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MSSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLT
WKLWEKFAPQNARLHYISCDKHPLKKDDLIKCLQKWPELSVQTEKLIEHYPVLTPGYHHLAFSNNRITLTLMLGDVLECY
EQLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATYSASSIVKTALTNAGFVINKRKG
FGPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKEERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQ
QAVLFPKLSIYKSPFTQFMLYSFLYANDVYKELLKHYNLGELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQS
SELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIDASVLILANGQQVNYFHETNH
LPVKAIRGQMTTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIIWSQNV
LDHWAGIRAASPDYLPIVGPLPNALEFKEIYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINRE
ISILPRKLIQAISPARFLRKKIIQGP

Sequences:

>Translated_666_residues
MSSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLT
WKLWEKFAPQNARLHYISCDKHPLKKDDLIKCLQKWPELSVQTEKLIEHYPVLTPGYHHLAFSNNRITLTLMLGDVLECY
EQLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATYSASSIVKTALTNAGFVINKRKG
FGPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKEERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQ
QAVLFPKLSIYKSPFTQFMLYSFLYANDVYKELLKHYNLGELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQS
SELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIDASVLILANGQQVNYFHETNH
LPVKAIRGQMTTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIIWSQNV
LDHWAGIRAASPDYLPIVGPLPNALEFKEIYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINRE
ISILPRKLIQAISPARFLRKKIIQGP
>Mature_665_residues
SSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNESNVFTIAETGFGTGMNFLLTW
KLWEKFAPQNARLHYISCDKHPLKKDDLIKCLQKWPELSVQTEKLIEHYPVLTPGYHHLAFSNNRITLTLMLGDVLECYE
QLLFCGDINLEHQLRESYVNAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATYSASSIVKTALTNAGFVINKRKGF
GPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKEERTALIVGGGLAGCFIANSLAKRGWEVTILEEKEKVGCGGSANQQ
AVLFPKLSIYKSPFTQFMLYSFLYANDVYKELLKHYNLGELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQSS
ELSGISLPCGGLFIPSSGWINSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIDASVLILANGQQVNYFHETNHL
PVKAIRGQMTTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDDQLNLDRLKRIAPDIIWSQNVL
DHWAGIRAASPDYLPIVGPLPNALEFKEIYSELKSNSKRWIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINREI
SILPRKLIQAISPARFLRKKIIQGP

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=665, Percent_Identity=33.3834586466165, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 75130; Mature: 74999

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNE
CCCCCEEEEEECCCCCCCCCCEEEECCEEEECCCCCCCEEEEEEECCHHHHHHHHCCCCC
SNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKHPLKKDDLIKCLQKWPELS
CCEEEEEECCCCCCCHHEEEEEHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
VQTEKLIEHYPVLTPGYHHLAFSNNRITLTLMLGDVLECYEQLLFCGDINLEHQLRESYV
CCHHHHHHHCCCCCCCCEEEEEECCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATYSASSIVKTALTNAGFVINKRKG
CEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCEEEECCCC
FGPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKEERTALIVGGGLAGCFIANSLAKRG
CCCCCCEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCC
WEVTILEEKEKVGCGGSANQQAVLFPKLSIYKSPFTQFMLYSFLYANDVYKELLKHYNLG
CEEEEEECCCCCCCCCCCCCCEEEEECHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQSSELSGISLPCGGLFIPSSGW
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
INSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIDASVLILANGQQVNYFHETNH
CCCCCCEEEEECCCEEEEEECCEEEECCCCCCCCEEEECCCEEEEEECCCEEEEEECCCC
LPVKAIRGQMTTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDD
CCHHHHCCCHHHHHCCCCCCEEEEEEECCCCEECCCCCCCCCCCEECCCCCCCCCCCCCC
QLNLDRLKRIAPDIIWSQNVLDHWAGIRAASPDYLPIVGPLPNALEFKEIYSELKSNSKR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCE
WIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINREISILPRKLIQAISPARFLRK
EEHHCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
KIIQGP
HHCCCC
>Mature Secondary Structure 
SSPFVPIITADIDWRDDLPYSLQFDDIYYSAEGGINQSLYVFVEGNNLINRWQQLPTNE
CCCCEEEEEECCCCCCCCCCEEEECCEEEECCCCCCCEEEEEEECCHHHHHHHHCCCCC
SNVFTIAETGFGTGMNFLLTWKLWEKFAPQNARLHYISCDKHPLKKDDLIKCLQKWPELS
CCEEEEEECCCCCCCHHEEEEEHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCC
VQTEKLIEHYPVLTPGYHHLAFSNNRITLTLMLGDVLECYEQLLFCGDINLEHQLRESYV
CCHHHHHHHCCCCCCCCEEEEEECCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
NAWYLDGFSPSKNQSMWSDNLFTVIAMLSKESTTVATYSASSIVKTALTNAGFVINKRKG
CEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCEEEECCCC
FGPKRHMICAHYEKAYSSSKKNRHTPWHINYPVTKEERTALIVGGGLAGCFIANSLAKRG
CCCCCCEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHCC
WEVTILEEKEKVGCGGSANQQAVLFPKLSIYKSPFTQFMLYSFLYANDVYKELLKHYNLG
CEEEEEECCCCCCCCCCCCCCEEEEECHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELKGSLLLAHNEREKANQQSLIHWLELYPELGQLVDEKQSSELSGISLPCGGLFIPSSGW
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
INSPELCDILIDNKRISLITGNRVQSINYNQKNWVVNDIDASVLILANGQQVNYFHETNH
CCCCCCEEEEECCCEEEEEECCEEEECCCCCCCCEEEECCCEEEEEECCCEEEEEECCCC
LPVKAIRGQMTTIQSTQESTKLKIPLCAEGHVLPALNNSHRVGASYDIGTSEPELNALDD
CCHHHHCCCHHHHHCCCCCCEEEEEEECCCCEECCCCCCCCCCCEECCCCCCCCCCCCCC
QLNLDRLKRIAPDIIWSQNVLDHWAGIRAASPDYLPIVGPLPNALEFKEIYSELKSNSKR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCE
WIAEAAPCYPNLYVCAAFGSRGLTTIPLATEWLAGLINREISILPRKLIQAISPARFLRK
EEHHCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
KIIQGP
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA