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The map label for this gene is pcnB [H]

Identifier: 54294419

GI number: 54294419

Start: 1655897

End: 1657168

Strand: Reverse

Name: pcnB [H]

Synonym: lpl1488

Alternate gene names: 54294419

Gene position: 1657168-1655897 (Counterclockwise)

Preceding gene: 54294422

Following gene: 54294418

Centisome position: 49.53

GC content: 38.92

Gene sequence:

>1272_bases
CTGGCTTATATCATCCCGAGAAATAAACATAACATATCGAAAGCAGATATAAGTAATAATGCGCTTAAAGTTCTTAACCG
TTTAATCAGCCATGGTTTTCAGGCCTATATCGTTGGAGGCAGTGTCAGAGATTTACTGCTCCATAAAGCGCCTAAAGATT
TTGATATCGCGACAAATGCGACTCCCAATGAGGTAAAAAATCTTTTCAAAAATGCTCGTATCATTGGTCGTCGATTTAAA
CTGGTACATATTTTATTTCATCGTGAAATTATTGAGGTAGCAACTTTTCGTGGCCATAATTCAAGCGATGCAAGCCACCA
ATTTAATGAACGCGGCATGCTTGTAAGAGATAATGTTTATGGCTCTCTGGACGAAGACGCATGGAGACGTGATTTCACAA
TAAACTCTCTTTATTATAATATTTCTGATTCTTCCATCATTGATTTTACTGGTGGTGTAGAAGACGTTAAGCAAAAGTTA
ATTCGAATAATTGGTGATCCCGTTACGCGTTATCAAGAGGATCCTGTCCGTATGTTACGTGCTGTCAGGTTTAGTGCCAA
GCTGCAATTTAATTTATCTCCGGAAACCAAAGCTCCCTTCCCCAAAATAAGCCATCTTATAACCCATGTTTCCAGTTCGC
GACTATTTGATGAAATAACGAAACTTTATCAATGCGGAGAAGCCGAATCGGTTCAAAAATTATTGGTTGAGTATGGTTTA
TTTAATTATCTATTTCCTCAAACAGCTGCTCTATTTGATAGTCATTACCCGGTTAATCCTCTTTTGGGCATAGCACTTGA
AAATACTGATACAAGAATACGTGATGGAAAGCCTGTTACTCCGGCATTTCTTATTGCTGTTTTGTTATGGTTTCCAATGA
TAGAGTGTTCCAGAAAACTGCAAGAATCTGGGCTTCCGCCTTTACCCTCGATTGAAAAAGCCATGTCTATAGTGCTTAGT
GAACAGAATGCAATTATTTCGATACCTAAAAGGTATAGCCAGGTTATAAGGGAAATTTGGCTTTTGCAATATCGTTTCGA
CAAAAGATTTGGTGGCAGAGCATTTAATTTACTTCTTCATCCCCGATTCAGAGCAGCGTATGATTTTATGGCTCTTAGAG
CATTATCTGGTGATGAGTCAATGGAGCTTGCTCAGTGGTGGGACACGTTTCAAGTAGTCGATGATGTTGAAAAAAATAAC
ATGGTAACTCAATTGGCTGCAACAACGCCCGGGCGGCCTAAAAAACGTCGTAAACGGAAAGTAGCAAAATGA

Upstream 100 bases:

>100_bases
CAACCGAATTACTGTTTAATCAATTAAGAGAGAAACAACAATCATCAAGTTTATTAAAAAACTGTTAAGAAGAAACAGGT
CGCATGGACATGCAATTGAC

Downstream 100 bases:

>100_bases
ATGTTTGCTATCTGAGCTTGGGATCGAATCAAAAAAATCCTGAGCGTCAAATAAGACAAGCCATTAAAGAAATCAAACAG
ATTCCATCAACATGCCTTAT

Product: Poly(A) polymerase (PAP) (plasmid copy number protein)

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFK
LVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKL
IRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGEAESVQKLLVEYGL
FNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLS
EQNAIISIPKRYSQVIREIWLLQYRFDKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWDTFQVVDDVEKNN
MVTQLAATTPGRPKKRRKRKVAK

Sequences:

>Translated_423_residues
MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFK
LVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKL
IRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGEAESVQKLLVEYGL
FNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLS
EQNAIISIPKRYSQVIREIWLLQYRFDKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWDTFQVVDDVEKNN
MVTQLAATTPGRPKKRRKRKVAK
>Mature_422_residues
AYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKNLFKNARIIGRRFKL
VHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKLI
RIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGEAESVQKLLVEYGLF
NYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSE
QNAIISIPKRYSQVIREIWLLQYRFDKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWDTFQVVDDVEKNNM
VTQLAATTPGRPKKRRKRKVAK

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=243, Percent_Identity=30.0411522633745, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081691, Length=411, Percent_Identity=49.8783454987835, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1789436, Length=231, Percent_Identity=29.8701298701299, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=284, Percent_Identity=28.5211267605634, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281360112, Length=211, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357337, Length=211, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 48509; Mature: 48377

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNA
CEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCC
TPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCC
GSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKLIRIIGDPVTRYQEDPVRMLR
CCCCHHHHHCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHH
AVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGEAESVQKLLVEYGL
HHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKL
HHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFDKRFGGRAFNLLLH
HHCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PRFRAAYDFMALRALSGDESMELAQWWDTFQVVDDVEKNNMVTQLAATTPGRPKKRRKRK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHC
VAK
CCC
>Mature Secondary Structure 
AYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNA
EEEECCCCCCCCHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCC
TPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCC
GSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKLIRIIGDPVTRYQEDPVRMLR
CCCCHHHHHCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHH
AVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGEAESVQKLLVEYGL
HHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKL
HHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFDKRFGGRAFNLLLH
HHCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PRFRAAYDFMALRALSGDESMELAQWWDTFQVVDDVEKNNMVTQLAATTPGRPKKRRKRK
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHC
VAK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]