Click here to switch to the map view.

The map label for this gene is thiDE [H]

Identifier: 54294388

GI number: 54294388

Start: 1624260

End: 1625726

Strand: Reverse

Name: thiDE [H]

Synonym: lpl1457

Alternate gene names: 54294388

Gene position: 1625726-1624260 (Counterclockwise)

Preceding gene: 54294389

Following gene: 54294387

Centisome position: 48.59

GC content: 38.92

Gene sequence:

>1467_bases
ATGAAAAAACCGATTGTTTGGACAATTGCTGGTGTTGACTCATCCGGATTAGCTGGTGTCCATGCTGACATGGAAACTTT
TAGTCGCTTGAATGTCAGGGCTTGTTCAGTCATCACCGCAGTAACTGCACAAAATGCTCACTCAATTACAGCAGTAGAAG
CGATATCCAGAGATCAGGTTGCTGCTCAGTGCCGTGTCTTGGAATTAAATTTAAAACCGGATGCCATTAAAATTGGTATG
TTATGTTCTACACCAATCTGTGAAGAAATAGCTTATTTTTTAAAGGGTTATGAAGGATTTGTTGTTCTTGATCCGATAAT
TACCTCCTCCTCGGGAACTAACCTGTTTTTTCCAGATTTACAACAGCATAAAAAAAATTTAATTCAATTGTTTCCGTACA
TTACTATTATTACTCCTAATCGGATAGAAGCAGAAATTATTTTAAATCGGAGCATTTCTTCTTATCAGGATATAATCAAT
GCTGCTTCAGATCTATTATCACTGGGAGCAAAGCAGGTATTACTGAAAGGAGGACATGTAAAAGATAACTCATTCAGTCA
AGATTACTGGACTGATGGAAAAGAGTCGTTTTGGATTGCAAATCATCGTTTTCCTGAAAGAAATTACCGTGGGACAGGCT
GTGCTCTATCTTCAGCACTCACAGCATGTCTTGCGCTTGGCTATTCAATGAAAGATGCTATCGTTATTGCTAAAATGTAT
GTCAGTCGCGGCATACGTCAATCAATAGAAATTGATAAAGAGGCCTCGCAACTTTATCATGATGGTTGGCCAGAAGATGA
GGCCGATCTCCCCTATTTATCACCAACACCGTTCATTAAACCAGTTCCTTCATTTAAAAAATATTCTATGGGTTTTTATC
CCATTGTGGATAGTAGCCACTGGTTAGAAATGCTTTTGCCATCGGGAATTAAGTGCATTCAGTTACGAATAAAGGAAACC
TCTCAAGAGCGGTTGGAAGAAGAGATAAAACGGAGTGTCCATTTGGCAAATCAGTATAATGCTGCATTATTTATTAATGA
TCACTGGGAACTTGCTATTCATTATGGTGCTGCAGGAGTTCACCTTGGCCAAGAGGATCTGGAAAAAGCTGATGTCGATA
GAATTAATCGAGCAGGTTTATTCTTAGGCATAAGTACGCATTGCTATTACGAAGTTGCTCGGGCTCATGCTCTTAACCCT
TCCTACGTCGCTTGTGGCCCCATATATGAAACAACCAGCAAAATAATGCCATTTCAAGCACAAGGAATTGCACGACTTGA
ACGTTGGCGAAAAACGCTTCGTTATCCATTGGTTGCCATTGGAGGTATAACTTTAAAGAACCTTTCTGATGTCTTAAAAA
CAAAAATTGATGGTGTGTCAGTGATCTCTGCCATTACAAAAGCATCAGCACCTTTAGTTGCAGCCAAACAATTTTTAACT
CAAATGAATGAATCACATAATGAATAA

Upstream 100 bases:

>100_bases
GTCATCAGGCATTTACAGGAGGAATGATGTCTGAGAGAAATGTAGCTCACCCAAGTACTCCATTAATCGATACTCCATTT
TGGCATCAGGTAAACAATTT

Downstream 100 bases:

>100_bases
ATCAACTTTGGCAATGAACGAATTAACCCGCTATTCTCAACAAATAAAGATGGAAGAAATTGGATTAGACGGACAGGAGA
AACTTAAGAATTCTCGAGTA

Product: hypothetical protein

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQVAAQCRVLELNLKPDAIKIGM
LCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKKNLIQLFPYITIITPNRIEAEIILNRSISSYQDIIN
AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANHRFPERNYRGTGCALSSALTACLALGYSMKDAIVIAKMY
VSRGIRQSIEIDKEASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPSGIKCIQLRIKET
SQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNP
SYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLVAAKQFLT
QMNESHNE

Sequences:

>Translated_488_residues
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQVAAQCRVLELNLKPDAIKIGM
LCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKKNLIQLFPYITIITPNRIEAEIILNRSISSYQDIIN
AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANHRFPERNYRGTGCALSSALTACLALGYSMKDAIVIAKMY
VSRGIRQSIEIDKEASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPSGIKCIQLRIKET
SQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNP
SYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLVAAKQFLT
QMNESHNE
>Mature_488_residues
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQVAAQCRVLELNLKPDAIKIGM
LCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKKNLIQLFPYITIITPNRIEAEIILNRSISSYQDIIN
AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKESFWIANHRFPERNYRGTGCALSSALTACLALGYSMKDAIVIAKMY
VSRGIRQSIEIDKEASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSHWLEMLLPSGIKCIQLRIKET
SQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNP
SYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLVAAKQFLT
QMNESHNE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=204, Percent_Identity=45.0980392156863, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788420, Length=265, Percent_Identity=35.4716981132075, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=28.9855072463768, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=269, Percent_Identity=32.7137546468402, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324517, Length=269, Percent_Identity=31.9702602230483, Blast_Score=94, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 54229; Mature: 54229

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AAQCRVLELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDL
HHEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCEEECCCCCEEECCCH
QQHKKNLIQLFPYITIITPNRIEAEIILNRSISSYQDIINAASDLLSLGAKQVLLKGGHV
HHHHHHHHHHCCEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
KDNSFSQDYWTDGKESFWIANHRFPERNYRGTGCALSSALTACLALGYSMKDAIVIAKMY
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSRGIRQSIEIDKEASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSH
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCH
WLEMLLPSGIKCIQLRIKETSQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGV
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCC
HLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNPSYVACGPIYETTSKIMPFQA
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCH
QGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLVAAKQFLT
HHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
QMNESHNE
HHHHCCCC
>Mature Secondary Structure
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AAQCRVLELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDL
HHEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCEEECCCCCEEECCCH
QQHKKNLIQLFPYITIITPNRIEAEIILNRSISSYQDIINAASDLLSLGAKQVLLKGGHV
HHHHHHHHHHCCEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
KDNSFSQDYWTDGKESFWIANHRFPERNYRGTGCALSSALTACLALGYSMKDAIVIAKMY
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
VSRGIRQSIEIDKEASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKYSMGFYPIVDSSH
HHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCH
WLEMLLPSGIKCIQLRIKETSQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGV
HHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCC
HLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNPSYVACGPIYETTSKIMPFQA
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCH
QGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASAPLVAAKQFLT
HHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHH
QMNESHNE
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]