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The map label for this gene is pyrF [H]

Identifier: 54294311

GI number: 54294311

Start: 1535735

End: 1536424

Strand: Direct

Name: pyrF [H]

Synonym: lpl1376

Alternate gene names: 54294311

Gene position: 1535735-1536424 (Clockwise)

Preceding gene: 54294310

Following gene: 54294312

Centisome position: 45.9

GC content: 40.29

Gene sequence:

>690_bases
ATGACACCTAAATTAATCGTTGCGTTGGATTTTGATAATCAAGATAACGCTTTACAGTTGGTTGAAAAACTTGATCCGAA
TCATTGCGCACTTAAGGTTGGGAGTGAGCTTTTTACTCTATTAGGGCCTCAGTTTGTAAAAGAATTGGTAAGAAGAGAGT
TTAAAGTATTTTTAGATTTAAAATTTCACGATATCCCTAATACAGTTGCTAAAGCATGCTACTCCGCTGCAGAATTAGGG
GTATGGATGATAAATGTTCATGCCATCGGCGGCTTAAAAATGTTGCAAGCTGCCAGGGAATCTTTAAAAACGTATGGTAA
AGACAGGCCTTTGTTGATTGCAGTGACTGTGTTGACCAGTTTTGAAGAAGGGGAGCTGGCCAGTGTTGGTATTAGGAATA
CATTGCCTGAGCAAGCAACTCATCTGGCTATGCTTGCCAGAGAGGCTGGGCTTGATGGTGTGGTAAGCTCTGCACATGAA
GTTAAAATTATAAAACAGAAGTGTGGTGAGAATTTTATTACCGTTACTCCTGGCATAAGACTGCCAAATAATCTAAAAGA
TGATCAATCGCGAGTAATGACTCCACAACAAGCCATCAGAGAAGGAAGTGATTTTCTGGTTATTGGAAGACCAATTACGC
AAGCGAGCAATCCTTATGAGGTTGTCTCAGCACTATTGCGTGATCTTTGA

Upstream 100 bases:

>100_bases
CCTGCCTATGCACCCTTATTTACAAGAAGATGAGCAATTGAAAATAGTTGCAGCAGTTAGAAATGCTTTACTTGTGATGA
ATGAAGAGGCCATGGTATAA

Downstream 100 bases:

>100_bases
TGGTGCATACTTTTAAAAAATTATACATATGGTATAATTTTGTTCATTTTTGAATTAGTTGTACCATTCCTATTTTATTC
CATAGAAATTTATGATAATT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDLKFHDIPNTVAKACYSAAELG
VWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGIRNTLPEQATHLAMLAREAGLDGVVSSAHE
VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL

Sequences:

>Translated_229_residues
MTPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDLKFHDIPNTVAKACYSAAELG
VWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGIRNTLPEQATHLAMLAREAGLDGVVSSAHE
VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL
>Mature_228_residues
TPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDLKFHDIPNTVAKACYSAAELGV
WMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGIRNTLPEQATHLAMLAREAGLDGVVSSAHEV
KIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=221, Percent_Identity=55.2036199095023, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25231; Mature: 25099

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDL
CCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEE
KFHDIPNTVAKACYSAAELGVWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
FEEGELASVGIRNTLPEQATHLAMLAREAGLDGVVSSAHEVKIIKQKCGENFITVTPGIR
CCCCCEEECCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL
CCCCCCCHHHHCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TPKLIVALDFDNQDNALQLVEKLDPNHCALKVGSELFTLLGPQFVKELVRREFKVFLDL
CCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEE
KFHDIPNTVAKACYSAAELGVWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
FEEGELASVGIRNTLPEQATHLAMLAREAGLDGVVSSAHEVKIIKQKCGENFITVTPGIR
CCCCCEEECCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL
CCCCCCCHHHHCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA