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The map label for this gene is mrdA [H]

Identifier: 54294261

GI number: 54294261

Start: 1482901

End: 1484754

Strand: Reverse

Name: mrdA [H]

Synonym: lpl1326

Alternate gene names: 54294261

Gene position: 1484754-1482901 (Counterclockwise)

Preceding gene: 54294262

Following gene: 54294260

Centisome position: 44.38

GC content: 40.18

Gene sequence:

>1854_bases
ATGTATCGGAATCAATCATTTAAAAACTATCGCGCTGAATCCCAACATCAACGATTTAGACTTAATCTATTAATTGCGAT
TCTGATTATTTTATCTTTAATACTGATTTTAAGATTGGCCTTCCTGCAAATATCCGAATTTAAACGATATCAGACCTTGT
CTTTAAAAAATCAAATGAGCGTGATACCTATTGCTCCTCCAAGAGGGGTTATTTTGGATAGAAATGGAGTGTTACTCGCT
GAAAACATACCAGTGTATGTGCTGGAAATCACTCCCGAACGTGTTAAGGACATGAAACAGACTTTGACTAAACTTCGAGA
GTTAATTCCTTCGATTACAGACGAAGATATTGATAGCTTCAATAAAACCAGATTACAAAATCGTTCTTTTGTTCCCATTC
CTATCAAATTAAAATTAAGCCAGGAAGAAGTAGCTACCTTTGCCAGCAATCAGTATCATTTCCCGGGAGTAAACATTAAA
GCGAGATTGATGCGTCATTACCCTCTTGGAGAGATTACTGCCCACGCCTTGGGGTATGTCGGCCGCATTAATATTCAGGA
ACTAAAACAAGTGGACTCCACTAATTATCGTGCTACCAACTTTATTGGCAAGGCCGGTATTGAAAAATACTATGAAGATA
TTCTCCATGGTAAAGTGGGTTATCAAATGGTAGAAACTGATGTTAGTGGCCGCACTTTAAGAATAATAAATAAAGTCAAT
CCTCATTCAGGAGCGAAACTGTATTTAAGCATAGACGCCAGACTTCAGGAAGCAGCTTATAATGCGTTGAAGGATAAACG
AGGTGCTGTAGTAGTCATAAACTCAAGGAATGGCGAAGTACTTGCGATGGTGAGTTCTCCAAGTTTTGATCCCAATATTT
TCGTCAGTGGAGTCAGTAAAGCGGATTATAAAGTGTTATCCAATGCCCTCCAGCGCCCTCTTTTTAATCGAGCAGTCCGA
GGTGTTTATCCCCCTGCATCAACAATCAAACCGTTTGTTGGACTGGCTGGTTTGGATAAAGGATTCATTACCACGACCAC
TGAAATATATGACCCCGGAAAGTACAAACTCCCTACTTCCAGCCATGTTTACCGTGATTGGAAAAAAACAGGCCACGGGG
TCATTAATTTCAAACGAGCCATTACCGTCTCTTGTGACACTTTCTTTTACCAACTAGGTAATAAAATGGGAATCTCTAAC
ATTGAAGACATGCTTGTCAAGTTTGGTTTGGGACAACTAACGCACGTTGACCTTCATGAAGAAGCTAATGGAGTAATACC
AAGTGCTCGCTGGAAAAGACAAACAAAAGGTGTATCATGGTATCCTGGAGATACTCTCATTTCTGCAATAGGGCAAGGAT
TTATGCTGGCAACTCCTTTGCAAATGGCTAATGCCACAGCCTCACTAAGTCAGCATGGACAACGATTCAGACCTCACCTA
CTGATAAAAACAGTAAACAGCGACACTAATGAGACCGAAGAATACAAACCATTTGAAGAATATCCAGTTTCCCTGAGAGA
TGAAGCAAATTGGGATGTTGTTATTGATGCGATGCATAGCGTGCTTACCAGCAATGAAGGAACCGGTTATCGTTTTGGCA
GAAATCCTCCCTACCCGGTAGCAGGAAAAACAGGTACAGCACAAGTATACAGTGGTAGACAATATGAAAAGGCAAAATAC
GAAGACATACCTGAACACTTGAGGGATAATTCGTTATTTATTGCATTTACTCCTGTAGAAAAACCGGAAATTGCCATTGC
CGTGGTGGTTGAAAATGATACCATCGCTTCCACTGTAGCACGTAAGGTGCTTGACACTTATTATCAACTTTATCCTATTA
AGAAAACACCATGA

Upstream 100 bases:

>100_bases
AATAAAAAACCACAAGCTTTATAAATATTTTATAATTGGTAAAAATCTGTAATTTCAGGTACTATTCCTACTTTTGTTTT
GTCATATAACATTTTCTGCG

Downstream 100 bases:

>100_bases
GTAGACAAACGACTAAGCCCGTATATCGTTTTACAGCAAAGTCCCTTCACCTGGATTTTCCATTACTGGGATTAATACTT
ACTCTTATTACCTTCGGACT

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQISEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLA
ENIPVYVLEITPERVKDMKQTLTKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVNIK
ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGKVGYQMVETDVSGRTLRIINKVN
PHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSRNGEVLAMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVR
GVYPPASTIKPFVGLAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQLGNKMGISN
IEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAIGQGFMLATPLQMANATASLSQHGQRFRPHL
LIKTVNSDTNETEEYKPFEEYPVSLRDEANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKY
EDIPEHLRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP

Sequences:

>Translated_617_residues
MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQISEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLA
ENIPVYVLEITPERVKDMKQTLTKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVNIK
ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGKVGYQMVETDVSGRTLRIINKVN
PHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSRNGEVLAMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVR
GVYPPASTIKPFVGLAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQLGNKMGISN
IEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAIGQGFMLATPLQMANATASLSQHGQRFRPHL
LIKTVNSDTNETEEYKPFEEYPVSLRDEANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKY
EDIPEHLRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP
>Mature_617_residues
MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQISEFKRYQTLSLKNQMSVIPIAPPRGVILDRNGVLLA
ENIPVYVLEITPERVKDMKQTLTKLRELIPSITDEDIDSFNKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVNIK
ARLMRHYPLGEITAHALGYVGRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGKVGYQMVETDVSGRTLRIINKVN
PHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSRNGEVLAMVSSPSFDPNIFVSGVSKADYKVLSNALQRPLFNRAVR
GVYPPASTIKPFVGLAGLDKGFITTTTEIYDPGKYKLPTSSHVYRDWKKTGHGVINFKRAITVSCDTFFYQLGNKMGISN
IEDMLVKFGLGQLTHVDLHEEANGVIPSARWKRQTKGVSWYPGDTLISAIGQGFMLATPLQMANATASLSQHGQRFRPHL
LIKTVNSDTNETEEYKPFEEYPVSLRDEANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPVAGKTGTAQVYSGRQYEKAKY
EDIPEHLRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVARKVLDTYYQLYPIKKTP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=614, Percent_Identity=42.9967426710098, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1786272, Length=605, Percent_Identity=25.2892561983471, Blast_Score=138, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69556; Mature: 69556

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQISEFKRYQTLSLKNQMS
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCE
VIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTLTKLRELIPSITDEDIDSF
EEEECCCCCEEECCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVNIKARLMRHYPLGEITAHALGYV
HHHHHCCCCCCEEEEEEEECHHHHHHHHCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHH
GRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGKVGYQMVETDVSGRTLRIINKVN
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCC
PHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSRNGEVLAMVSSPSFDPNIFVSGVSK
CCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCH
ADYKVLSNALQRPLFNRAVRGVYPPASTIKPFVGLAGLDKGFITTTTEIYDPGKYKLPTS
HHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEHEEECCCCEECCCC
SHVYRDWKKTGHGVINFKRAITVSCDTFFYQLGNKMGISNIEDMLVKFGLGQLTHVDLHE
HHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECHH
EANGVIPSARWKRQTKGVSWYPGDTLISAIGQGFMLATPLQMANATASLSQHGQRFRPHL
CCCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCEE
LIKTVNSDTNETEEYKPFEEYPVSLRDEANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPV
EEEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCC
AGKTGTAQVYSGRQYEKAKYEDIPEHLRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVA
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHH
RKVLDTYYQLYPIKKTP
HHHHHHHHHEECCCCCC
>Mature Secondary Structure
MYRNQSFKNYRAESQHQRFRLNLLIAILIILSLILILRLAFLQISEFKRYQTLSLKNQMS
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCE
VIPIAPPRGVILDRNGVLLAENIPVYVLEITPERVKDMKQTLTKLRELIPSITDEDIDSF
EEEECCCCCEEECCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NKTRLQNRSFVPIPIKLKLSQEEVATFASNQYHFPGVNIKARLMRHYPLGEITAHALGYV
HHHHHCCCCCCEEEEEEEECHHHHHHHHCCCEECCCCCHHHHHHHCCCCHHHHHHHHHHH
GRINIQELKQVDSTNYRATNFIGKAGIEKYYEDILHGKVGYQMVETDVSGRTLRIINKVN
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCC
PHSGAKLYLSIDARLQEAAYNALKDKRGAVVVINSRNGEVLAMVSSPSFDPNIFVSGVSK
CCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCCEEEECCCH
ADYKVLSNALQRPLFNRAVRGVYPPASTIKPFVGLAGLDKGFITTTTEIYDPGKYKLPTS
HHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEHEEECCCCEECCCC
SHVYRDWKKTGHGVINFKRAITVSCDTFFYQLGNKMGISNIEDMLVKFGLGQLTHVDLHE
HHHHHHHHHCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECHH
EANGVIPSARWKRQTKGVSWYPGDTLISAIGQGFMLATPLQMANATASLSQHGQRFRPHL
CCCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCEE
LIKTVNSDTNETEEYKPFEEYPVSLRDEANWDVVIDAMHSVLTSNEGTGYRFGRNPPYPV
EEEEECCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCC
AGKTGTAQVYSGRQYEKAKYEDIPEHLRDNSLFIAFTPVEKPEIAIAVVVENDTIASTVA
CCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHH
RKVLDTYYQLYPIKKTP
HHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]