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The map label for this gene is pilS [H]

Identifier: 54294117

GI number: 54294117

Start: 1318710

End: 1320296

Strand: Direct

Name: pilS [H]

Synonym: lpl1181

Alternate gene names: 54294117

Gene position: 1318710-1320296 (Clockwise)

Preceding gene: 54294116

Following gene: 54294118

Centisome position: 39.42

GC content: 35.1

Gene sequence:

>1587_bases
ATGTCTGCTGCTCGCCAAATCAACAATCCGAAAATGAGACAATGGCGAATATTGTTGGTTTACAATATTTACAGAATAGT
AAGTATTTTTCTATTTTTAGGCATTTATTATTTCAGTTATATCAATAAATATTATCCCTTATTATTTCTCTGTATCGCTT
TTGCCTATTTAGTATTTGCTTTGATTTTTCTTTATTTTTGGCATAGAAAAATTTTAAGTTTTGATAAGCAGGTGTTTTTA
TCGGGTACGATTGATGTCATAGCCATTTCCAGCATGCTAGGCATCATTGGTAATTTGGAATCCGGTTATGGCATATTACT
AAATGTTACTATTGCTGCTTTAAGTATTTTAGTTCCTGGGCGCTTGGCCATCTATTTTGCATCACTAGCCAGTTGTTTAT
TGTTATGTGGCAATGTTTTGCAATTTTTTATTTATAATCAAAAAGACTTAAGCACTTTTTTTTATAGTGGAATCTATGGG
GCTGGTTTTTTTGCAACGGCTATCACCGCATGGTATTTATCCAATTGGGTCAGAATGTCTGAGAGTCTGGCCAGACACCG
TAGCTATGAGTTGGCAGGAATGCAAAGGCTCAATGAATATATTGTCGAGCGATTGCATTCAGGCATTATTTATGTTGCAG
AGGGAAAGCGAATTCGATTGATGAATACTGCTGCCCGTGAATTTTTTAATCTGAGTAAACATCATGCAATTCAAAAATTG
GACCAGCTATCTGATTCACTTGTATCCAAATTTGATGATTTTCTTTTAAAAACAACGAATAATGAACGTACAGCTCAAAC
TATTATTGAAGATCCTTATCTAAGAGTACATTTTTTTTCTACTTCTGTTGGTCACAAGTCTGAAGTACTCATTATTCTGG
AAGACATGACCTATATTGCACAACAAGCACAACAACTCAAATTAGCGGCTTTAGGGCGTTTTTCGGCAAGTATTGCTCAT
GAGTTACGTAATCCCCTGGGGGCAATTGCTCATGCTATTCAATTGTTTGGAGATAATGAGGATTTAAATGAAGAGAACAC
TCGTTTAAAACAATTAATCATGAACAATTGTGAGCGAATGAACAGGGTGATTAAAAATGTATTGCAGCTTTCAAGGCGTG
AGAAATCTCAACCGCAGGTTAATGAACTTACAACATTTTTAGAACATTTTAAGCAGGATTTTACTCACAATAATCCCTGT
GATCTTACGATAAAACTACCTGTAAATAAGCCATTATCAGTTGTTTTTGATAAAAGTCAATTAGAACAGATTTTAGTAAT
CTTGTGTGAAAATGCTATTCAACATGGGCGGGATCATGAGGGTAATGTGAATATCGTCATTGCTGCCAAATGTTCTTCGA
ATAAAATCACTTTGACCGTATGTGATTCTGGACCTGGTATACCCTCTGAGCACAGAGACAATATATTTGAACCATTTTTT
ACCACCTTGCGAGGTGGTACAGGGATGGGGTTATTTATTGCCAGAGATTTGTGTGAAATTAATCAAGCCAGGTTAAATTT
GGTAAAATCAAATAAAGGATGTTGTTTTGCGATCACTGTTAATCCATCGGATGAACTTTTAATATGA

Upstream 100 bases:

>100_bases
AGGGATCATTGGTGTGGGTCTTAGTTGATGTATTGTGCAAAATGCTCAAGTCTGGTTATATAATTATAATACGCTGAATA
ATCTAATGGATTAGAAAAGG

Downstream 100 bases:

>100_bases
CCAAAAGTAAAGTGCTTGTTATAGATGATGAACCAGATATAAGAGAGTTATTAACTCTTACTTTATCACGCATGGGTTTA
ACTTGCGATGCAGTGTCTGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFL
SGTIDVIAISSMLGIIGNLESGYGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAH
ELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELTTFLEHFKQDFTHNNPC
DLTIKLPVNKPLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAKCSSNKITLTVCDSGPGIPSEHRDNIFEPFF
TTLRGGTGMGLFIARDLCEINQARLNLVKSNKGCCFAITVNPSDELLI

Sequences:

>Translated_528_residues
MSAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFL
SGTIDVIAISSMLGIIGNLESGYGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYG
AGFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAH
ELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELTTFLEHFKQDFTHNNPC
DLTIKLPVNKPLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAKCSSNKITLTVCDSGPGIPSEHRDNIFEPFF
TTLRGGTGMGLFIARDLCEINQARLNLVKSNKGCCFAITVNPSDELLI
>Mature_527_residues
SAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFALIFLYFWHRKILSFDKQVFLS
GTIDVIAISSMLGIIGNLESGYGILLNVTIAALSILVPGRLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYGA
GFFATAITAWYLSNWVRMSESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKLD
QLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIAQQAQQLKLAALGRFSASIAHE
LRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERMNRVIKNVLQLSRREKSQPQVNELTTFLEHFKQDFTHNNPCD
LTIKLPVNKPLSVVFDKSQLEQILVILCENAIQHGRDHEGNVNIVIAAKCSSNKITLTVCDSGPGIPSEHRDNIFEPFFT
TLRGGTGMGLFIARDLCEINQARLNLVKSNKGCCFAITVNPSDELLI

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=31.1627906976744, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788549, Length=356, Percent_Identity=25.561797752809, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693157, Length=356, Percent_Identity=25.2808988764045, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=247, Percent_Identity=26.7206477732794, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=27.9475982532751, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=249, Percent_Identity=26.1044176706827, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790300, Length=263, Percent_Identity=23.9543726235741, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1786912, Length=249, Percent_Identity=25.7028112449799, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790346, Length=194, Percent_Identity=27.319587628866, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60100; Mature: 59968

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
SAARQINNPKMRQWRILLVYNIYRIVSIFLFLGIYYFSYINKYYPLLFLCIAFAYLVFA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIFLYFWHRKILSFDKQVFLSGTIDVIAISSMLGIIGNLESGYGILLNVTIAALSILVPG
HHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCH
RLAIYFASLASCLLLCGNVLQFFIYNQKDLSTFFYSGIYGAGFFATAITAWYLSNWVRMS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESLARHRSYELAGMQRLNEYIVERLHSGIIYVAEGKRIRLMNTAAREFFNLSKHHAIQKL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHCCHHHHHHHHH
DQLSDSLVSKFDDFLLKTTNNERTAQTIIEDPYLRVHFFSTSVGHKSEVLIILEDMTYIA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEHHHHHHH
QQAQQLKLAALGRFSASIAHELRNPLGAIAHAIQLFGDNEDLNEENTRLKQLIMNNCERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NRVIKNVLQLSRREKSQPQVNELTTFLEHFKQDFTHNNPCDLTIKLPVNKPLSVVFDKSQ
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHEEECHHH
LEQILVILCENAIQHGRDHEGNVNIVIAAKCSSNKITLTVCDSGPGIPSEHRDNIFEPFF
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCHHHCCHHHHHH
TTLRGGTGMGLFIARDLCEINQARLNLVKSNKGCCFAITVNPSDELLI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]