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The map label for this gene is artJ [H]

Identifier: 54294066

GI number: 54294066

Start: 1256683

End: 1257378

Strand: Direct

Name: artJ [H]

Synonym: lpl1130

Alternate gene names: 54294066

Gene position: 1256683-1257378 (Clockwise)

Preceding gene: 54294063

Following gene: 54294067

Centisome position: 37.56

GC content: 34.2

Gene sequence:

>696_bases
ATGATTCTAAAGCCGGGAAAATTATTGGTGGCTACTGCCTATCCAGATCCACCGTTTGATATTGAGCCAGGTGATGGTTT
TGATGGTGATTTAATAAAAGAAATTTGTCGAATTTTAAATTTGCAGCTAGTCCAGATCAAATATGAGGGAGAAAATTTTA
ATGATATTTTTTTAGGCTTGGCTAATAAAGAATACGATGTTGTCATTTCAGGAACGACTATTACACCAAACCGATCAAAA
CAAGTACTTTTTTCGAATCCCTATTTAGAGTTTAATCAGGGTATCGCGGTTAATACTGAATTAAAGCCAAATGTTTCATC
TTTTGAAGATTTGAAAGGTTTAGTGGCTGGTATACAGAAAGGCAACACATCAGATGAAGTTGCTCAGGATTTATTACGTA
AGAAAATACTCAGTGGTATTAAATATTATGCATACAATGAAATAGAATTAGCCATACATGATCTGTCTGCCGGTAAAATT
GGTTTGGTCATTAAGTTATATCCTGTTATTTCCTGTTTAATTCGAAACTATACAAACCTGGCTGTTCCTTTGCAGGCTGA
AACACATGAAAAACTTGGTATCGCGGTTGCTAAAGATAATTTTAAACTACGGGATAATGTAAATCTTATTTTGGCCGATC
TTATACAAAATGGTACTTTAAGTCGTCTGCAACATAAGTGGATAAATACAACCTAG

Upstream 100 bases:

>100_bases
GTAAATTATTCATTTAAATCAATGGCATAAAAAATATTTGGCTGAATTTTTTCTGATTAATCTATACTTAGTGAATCGAT
TCCAAACAATAGTTATATCT

Downstream 100 bases:

>100_bases
GGTAAATTAGGCCAATGTCAGTTTCAACTCAGGAGATAACCTTATGTAAAGACAGGACTTACGATAAACTCCAATCTCTT
TGTTAAAAAAAGTTTTTAAC

Product: hypothetical protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKKILSGIKYYAYNEIELAIHDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDNVNLILADLIQNGTLSRLQHKWINTT

Sequences:

>Translated_231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKKILSGIKYYAYNEIELAIHDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDNVNLILADLIQNGTLSRLQHKWINTT
>Mature_231_residues
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGLANKEYDVVISGTTITPNRSK
QVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQKGNTSDEVAQDLLRKKILSGIKYYAYNEIELAIHDLSAGKI
GLVIKLYPVISCLIRNYTNLAVPLQAETHEKLGIAVAKDNFKLRDNVNLILADLIQNGTLSRLQHKWINTT

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=31.2217194570136, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787031, Length=232, Percent_Identity=31.4655172413793, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=26.8398268398268, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 25701; Mature: 25701

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEE
ANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQK
CCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHC
GNTSDEVAQDLLRKKILSGIKYYAYNEIELAIHDLSAGKIGLVIKLYPVISCLIRNYTNL
CCCHHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCE
AVPLQAETHEKLGIAVAKDNFKLRDNVNLILADLIQNGTLSRLQHKWINTT
EEEEECCCCCCEEEEEECCCEEEECCCCEEEEHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MILKPGKLLVATAYPDPPFDIEPGDGFDGDLIKEICRILNLQLVQIKYEGENFNDIFLGL
CCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEEEECCCCCCEEEEEE
ANKEYDVVISGTTITPNRSKQVLFSNPYLEFNQGIAVNTELKPNVSSFEDLKGLVAGIQK
CCCCEEEEEECCEECCCCCCEEEEECCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHC
GNTSDEVAQDLLRKKILSGIKYYAYNEIELAIHDLSAGKIGLVIKLYPVISCLIRNYTNL
CCCHHHHHHHHHHHHHHHCCCEEEECEEEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCE
AVPLQAETHEKLGIAVAKDNFKLRDNVNLILADLIQNGTLSRLQHKWINTT
EEEEECCCCCCEEEEEECCCEEEECCCCEEEEHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]