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The map label for this gene is mltD [H]

Identifier: 54294063

GI number: 54294063

Start: 1253376

End: 1254704

Strand: Direct

Name: mltD [H]

Synonym: lpl1127

Alternate gene names: 54294063

Gene position: 1253376-1254704 (Clockwise)

Preceding gene: 54294062

Following gene: 54294066

Centisome position: 37.46

GC content: 38.53

Gene sequence:

>1329_bases
TTGATTAAGACATTCATCGATAAAAAAATACAGCTGCTATTCAGTTGCCTTATTTTTTTTATCTTCAGTACTCATGCTAA
ATCATTTGAAACACCTGATGTATGGGATGTTTTGCGCAGTGAATTCAAGATGAATCATGAAGCCTATCGCCCAGAGGTTC
AAGATCAAATTCGCTGGCTACTGGATCATCCAGGGTTTGTGACTAAGGCCTGTAAACAGGCTGAGCCTTATCTTTATCAT
GTAACTCGAGAAATACAAAGAAGAAATTTACCCGGTGAGTTGGCATTACTCCCTATGATAGAAAGCGCTTACGATCCTTT
TGCTTACTCAAAAGTTGGTGCTGCAGGTTTATGGCAAATAATGCCGGTGACGGGGAATCAATTAGGTTTAATTCAAGATT
GGTGGTTTGATGGAAGACGAAGCATTAATCATTCAACTGATGCAGCCCTGACTCACCTGATGCATTTAAATAAAGTGTTT
AACGGAAGCTGGATACTTTCCATCGCAGCTTACGATGCTGGCGAAGGAGCCATAGGCAGAGCTGTAAAAGCAAATAACAC
TAACGGAGGTAGCGTCAGTTTTTGGAATCTGGATGTACCTAAAGAAACTCAAATTTATGTCCCTCGATTTTTAGCTTTGG
CTGAAATCATGAGCAATCCTCGGGCTTATAAAATAAATCTTCCTGCAATGCCATTTCGACCTTATTTTGAAGAGGTGAAT
ATTGGTAGTCAAATTGACTTGAATCATGCGGCTAGTTTAGCAGGAATTTCGGTTAGTGAATTAACCAAATTAAATCCGGG
TTATAATAGATGGGCTACCGCACCCTATAAACCCTTTACTTTGCTTATCCCTATAGCAAAAGTCACTCAATTTTATTTCA
ATTTATCCCATTCACCTGTTGATAAACGAGTGAGCTGGAGAAAACATCTCGTCCTTCCGAGTGACAGTTTACAAAGTATT
GCCAGAAAATATCATACGACTGTTTCATTAATAAAAAAATTAAATCGTCTGTCAGATCACTCGATAAGACCCAATCAGAT
CTTGTTAATTCCGAGCGCCAAAAGTACTCCAGCATTGCCTTTCCACGAAATTTCAAAGTCAAAATTATTAAGCCAGACAC
CAACAAAAGGTAAAGTATACAGGGTCATACATATTGTCCAAGCCAATGAAACGTATAATACTCTTGAGCGACTTTATAAA
GTTACAGCTCAGGATATTATGAAATGGAATCATATTGGCCCGGGAGAAGCGTTGACAAAAGGGCGACAGTTAATTATTTG
GAAAAAGTCAAGCAATCTTGAATGGAACAGGACTTTCAAAAAACCTTAA

Upstream 100 bases:

>100_bases
GAGTATATTTTATAAGACGCGCTCAGGGCCGGTTCAGGCGAAGAGAAACAATACATTATAAACGGGCTCCAGAGAAGATT
TATAGAGCAGGAATAGTAAT

Downstream 100 bases:

>100_bases
GTTTCCGGTAATCCTGCATTCTAAACATAACCCGTATTCGGTCAAAGTTTCACGCTTGCCTATTCGATAACTTATTAATA
AATTTGGTTAAATTTCTCAT

Product: hypothetical protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYH
VTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
NGSWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSI
ARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPALPFHEISKSKLLSQTPTKGKVYRVIHIVQANETYNTLERLYK
VTAQDIMKWNHIGPGEALTKGRQLIIWKKSSNLEWNRTFKKP

Sequences:

>Translated_442_residues
MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYH
VTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
NGSWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSI
ARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPALPFHEISKSKLLSQTPTKGKVYRVIHIVQANETYNTLERLYK
VTAQDIMKWNHIGPGEALTKGRQLIIWKKSSNLEWNRTFKKP
>Mature_442_residues
MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWLLDHPGFVTKACKQAEPYLYH
VTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQIMPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVF
NGSWILSIAAYDAGEGAIGRAVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPVDKRVSWRKHLVLPSDSLQSI
ARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPALPFHEISKSKLLSQTPTKGKVYRVIHIVQANETYNTLERLYK
VTAQDIMKWNHIGPGEALTKGRQLIIWKKSSNLEWNRTFKKP

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=408, Percent_Identity=31.3725490196078, Blast_Score=228, Evalue=5e-61,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 50498; Mature: 50498

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHH
LDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQI
HCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHCCCCCCEEE
MPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVFNGSWILSIAAYDAGEGAIGR
EECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
EEECCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCC
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPV
CCCEECCHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCH
DKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPALP
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
FHEISKSKLLSQTPTKGKVYRVIHIVQANETYNTLERLYKVTAQDIMKWNHIGPGEALTK
HHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
GRQLIIWKKSSNLEWNRTFKKP
CCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MIKTFIDKKIQLLFSCLIFFIFSTHAKSFETPDVWDVLRSEFKMNHEAYRPEVQDQIRWL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHCCCCHHHHHHHH
LDHPGFVTKACKQAEPYLYHVTREIQRRNLPGELALLPMIESAYDPFAYSKVGAAGLWQI
HCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHCCCCCCEEE
MPVTGNQLGLIQDWWFDGRRSINHSTDAALTHLMHLNKVFNGSWILSIAAYDAGEGAIGR
EECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
AVKANNTNGGSVSFWNLDVPKETQIYVPRFLALAEIMSNPRAYKINLPAMPFRPYFEEVN
EEECCCCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCC
IGSQIDLNHAASLAGISVSELTKLNPGYNRWATAPYKPFTLLIPIAKVTQFYFNLSHSPV
CCCEECCHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCH
DKRVSWRKHLVLPSDSLQSIARKYHTTVSLIKKLNRLSDHSIRPNQILLIPSAKSTPALP
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
FHEISKSKLLSQTPTKGKVYRVIHIVQANETYNTLERLYKVTAQDIMKWNHIGPGEALTK
HHHHHHHHHHHCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHC
GRQLIIWKKSSNLEWNRTFKKP
CCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]