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The map label for this gene is pdxJ [H]

Identifier: 54293919

GI number: 54293919

Start: 1094583

End: 1095329

Strand: Direct

Name: pdxJ [H]

Synonym: lpl0975

Alternate gene names: 54293919

Gene position: 1094583-1095329 (Clockwise)

Preceding gene: 54293918

Following gene: 54293920

Centisome position: 32.72

GC content: 42.7

Gene sequence:

>747_bases
ATGAATAAAGAGTTATTGTTAGGTGTTAATATTGATCATATAGCAACCATAAGACAAGCAAGAGGAACTCGTTATCCTGA
TCCTGTCCAGGCAGCAATGGATGCTGAAGAAGCTGGGGCAGATGGAATTACTTTGCATATGAGAGAGGACTTACGACATA
TTCAGGCGCGTGATGTCAGATTGATTAAACAGGTTCTGCAAACACGTATGAATCTTGAGCTGGCGGTTACTGAAGCCATG
CTTGATTTTGCCGAGGAAATATCGCCGGAGCATTCTTGCTTAGTGCCTGAAAAGCGAGAAGAACTAACTACAGAAGGTGG
ATTGGATATTTTGACTCACCGAAATGTGGTGGAAAAGGCAGTACGTCGCTTACAATTAATGGGAAGTGAGGTTTCTTTAT
TTATTGACCCGGATAAAGAGCAAATAAGAGCGGCGGTTGATGTTGGGGCGCCAGTTATCGAATTGCACACGGGATGTTAT
GCTGATGCCACCTCAGAGGAAAAGCAGCATTATGAATTGCAACGAATTAAAGAGGCAGCTGAATTTGCTGCCAGTTTGAA
CTTGGTAGTGAATGCCGGTCATGGATTGCACTATCATAATGTTAAACCGATTGCAGCGATTAGGGAGTTAAATGAACTTA
ACATAGGACATGCCATTATTGCCAGAGCATTATTCTGTGGATTAAAAGAGGCAGTAAGACACATGCGCCAATTAATGCAG
GAAGCGAGACTTTATGTCAACGACTGA

Upstream 100 bases:

>100_bases
CTTTAGATAGCGCCGTTTAAACTGATTGAAACAAGGTAACGTGAATAGCAGTTTGTCAAGAACTGCATTATACTCTGCAT
TTTATATTTTTTGGTGCAAA

Downstream 100 bases:

>100_bases
TGTCATTGTTGTACGTATCACTGGCGATTCTGGTGACGGAGTACAATTGGTAGGTGAACAATTAACCATTAGCGCGGCAT
TGACAGGACGTGATGTGCGT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAM
LDFAEEISPEHSCLVPEKREELTTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
EARLYVND

Sequences:

>Translated_248_residues
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAM
LDFAEEISPEHSCLVPEKREELTTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
EARLYVND
>Mature_248_residues
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVRLIKQVLQTRMNLELAVTEAM
LDFAEEISPEHSCLVPEKREELTTEGGLDILTHRNVVEKAVRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCY
ADATSEEKQHYELQRIKEAAEFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
EARLYVND

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=240, Percent_Identity=67.0833333333333, Blast_Score=323, Evalue=7e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 27845; Mature: 27845

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVR
CCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHCHHHCCCCCEEEEEHHHHHHHHHHHHH
LIKQVLQTRMNLELAVTEAMLDFAEEISPEHSCLVPEKREELTTEGGLDILTHRNVVEKA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
VRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCYADATSEEKQHYELQRIKEAA
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
EFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
HHHHHHHHEEECCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EARLYVND
HHHHCCCC
>Mature Secondary Structure
MNKELLLGVNIDHIATIRQARGTRYPDPVQAAMDAEEAGADGITLHMREDLRHIQARDVR
CCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHCHHHCCCCCEEEEEHHHHHHHHHHHHH
LIKQVLQTRMNLELAVTEAMLDFAEEISPEHSCLVPEKREELTTEGGLDILTHRNVVEKA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHH
VRRLQLMGSEVSLFIDPDKEQIRAAVDVGAPVIELHTGCYADATSEEKQHYELQRIKEAA
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHH
EFAASLNLVVNAGHGLHYHNVKPIAAIRELNELNIGHAIIARALFCGLKEAVRHMRQLMQ
HHHHHHHHEEECCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EARLYVND
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA