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The map label for this gene is nadB [H]

Identifier: 54293777

GI number: 54293777

Start: 943913

End: 945562

Strand: Reverse

Name: nadB [H]

Synonym: lpl0833

Alternate gene names: 54293777

Gene position: 945562-943913 (Counterclockwise)

Preceding gene: 54293778

Following gene: 54293776

Centisome position: 28.26

GC content: 44.06

Gene sequence:

>1650_bases
ATGAGTGATCAGCTATCACAACAAGGCCAATCGTTCGAATTCGATGTTCTCGTCATTGGGACAGGGTTAGCAGGGTTACA
TTATTGTCTGCAGCTTTTAGCTATCCAACCCAGGCTAAACATTGCGTTAATCAGCAAAGCAGAGTCCATTGAGTGTAACA
GTCGCTATGCTCAAGGAGGGATTGCTGCTGCCATTTCGCCAGAAGATTCCCTAGAGTCTCATATATCTGATACTGTATTG
GCTGGAGATGGGCTATGCTACTTACCTGCGGTGGAATTTATTATCCGCCAAGGGCCAGATATTATAAAACAACTTGATCA
GTATAAAGTTCAATTTAAAAGAGAAAGTGATGGTCACTTTCATCTTGCCAAAGAAGGTGGCCATTCACACCGGCGCATTT
TTAATTGTGGCGATCAAACAGGATTAACGCTAACACAAACTCTCAATCATTTGGCAAGGCAACATCAACAGATCCAGTTT
TTTGAACATCACGTGGCGGTTAACCTGATTACTCAATATCATCCTCACCGGACAGATAACCAGGGTGAGGTATTAGGAGC
CTATATACTCGACTGCCAACGTAATCGCATTCACACCTTTCTTGCGAAATGCGTTATTTTAGCGACCGGTGGCGCCGGCA
AAACCTACAGGTATACGACTAACCCCATGGTTGCAACAGGTGATGGTGTTGCGATGGCTTACAGGGCTGGAGCTCGTGTG
GGTAACATGGAATTTTATCAATTTCACCCTACTCTCTTGCACCATCACACTATTAACAATTTTTTAATTTCTGAAGCAGT
TCGAGGCGAAGGCGCTTTGCTTAAAAACCCCGAAACAGGCGAACGTTTTATGAAACGATACGCGCCTGACCAACTTGAGT
TAGCAACCAGAGATGTCGTCGCAAGAGCTATTTTTAGTGAAATTGAACAAGGCCAGGCTGGATATGTGCATCTAGATATT
TCTCATCAAAGCAAATCTTTCCTGAAAAAACGTTTCCCACAAATTTATTCCACATTGTTATCTATTGGAATTGATATGAG
TCAGGATTTAATTCCTGTCGTTCCGGCCGCCCATTACCAATGCGGTGGGGTTCTTACTGATGTGGACGGCCGCACCGATC
TGGCACGATTATACGCTATCGGTGAAGTGGCTTTTACTGGGTTGCATGGTGCGAACAGGCTGGCAAGCAATTCCCTACTT
GAAGCCTTGGTCATGGGTTCCAATGCGGCACGCTGTACACTCAAAGACATTGCTACTCCGCTGAAACTTATCGATCATAT
CCCCAGCTGGAGCTCTCCAGGAGAAGTCAATGCAAGAAGAGCCAGTCAAATCAACGCTCAATGGAGAGGCTTAAGAGGGG
AAATGACCTCCTATGCAGGCATTGTGCGCACTGAAGCAGGCCTTGAAGATCTACTACAATTAATATTGAAGCGTAAGAAA
ATTATTGAAGAGTATTACTGGAAACATTGCATTACCAGAGATTTTATTGAGTTAAGGAACATTATCCTTAATGCAGAATT
GATTGTTAGAGCAGCCCTTTCCAGAAGGGAATCACGTGGCGGACATTTTCGTGAAGATTTCCCCAATAAAAATTTCAAAG
CCCAGGAAAGTATTGCAAAACTGGGACAGGCAGACAGTTCATTAGGATAG

Upstream 100 bases:

>100_bases
TTAAAACCCTGTCTATTCCCGAAGAAGCAAAATCAGAATTAATGAAATTAACTCCAGAGACTTACACCGGTCTTGCGACC
CAATTGGTCAAGGCTTTTTC

Downstream 100 bases:

>100_bases
CTTGGTAAAAGCTATCCCATTAACAATAATGAAAGAACCCTTCCCACAAATCCCTAATGACTAGAGGTAGATTTAATGTT
TAAAAATTCAACTACTTACC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPRLNIALISKAESIECNSRYAQGGIAAAISPEDSLESHISDTVL
AGDGLCYLPAVEFIIRQGPDIIKQLDQYKVQFKRESDGHFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQRNRIHTFLAKCVILATGGAGKTYRYTTNPMVATGDGVAMAYRAGARV
GNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKNPETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDI
SHQSKSFLKKRFPQIYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGANRLASNSLL
EALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRGLRGEMTSYAGIVRTEAGLEDLLQLILKRKK
IIEEYYWKHCITRDFIELRNIILNAELIVRAALSRRESRGGHFREDFPNKNFKAQESIAKLGQADSSLG

Sequences:

>Translated_549_residues
MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPRLNIALISKAESIECNSRYAQGGIAAAISPEDSLESHISDTVL
AGDGLCYLPAVEFIIRQGPDIIKQLDQYKVQFKRESDGHFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQF
FEHHVAVNLITQYHPHRTDNQGEVLGAYILDCQRNRIHTFLAKCVILATGGAGKTYRYTTNPMVATGDGVAMAYRAGARV
GNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKNPETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDI
SHQSKSFLKKRFPQIYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGANRLASNSLL
EALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRGLRGEMTSYAGIVRTEAGLEDLLQLILKRKK
IIEEYYWKHCITRDFIELRNIILNAELIVRAALSRRESRGGHFREDFPNKNFKAQESIAKLGQADSSLG
>Mature_548_residues
SDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPRLNIALISKAESIECNSRYAQGGIAAAISPEDSLESHISDTVLA
GDGLCYLPAVEFIIRQGPDIIKQLDQYKVQFKRESDGHFHLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQFF
EHHVAVNLITQYHPHRTDNQGEVLGAYILDCQRNRIHTFLAKCVILATGGAGKTYRYTTNPMVATGDGVAMAYRAGARVG
NMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKNPETGERFMKRYAPDQLELATRDVVARAIFSEIEQGQAGYVHLDIS
HQSKSFLKKRFPQIYSTLLSIGIDMSQDLIPVVPAAHYQCGGVLTDVDGRTDLARLYAIGEVAFTGLHGANRLASNSLLE
ALVMGSNAARCTLKDIATPLKLIDHIPSWSSPGEVNARRASQINAQWRGLRGEMTSYAGIVRTEAGLEDLLQLILKRKKI
IEEYYWKHCITRDFIELRNIILNAELIVRAALSRRESRGGHFREDFPNKNFKAQESIAKLGQADSSLG

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=531, Percent_Identity=32.015065913371, Blast_Score=229, Evalue=6e-60,
Organism=Escherichia coli, GI1788928, Length=541, Percent_Identity=44.731977818854, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1790597, Length=553, Percent_Identity=32.368896925859, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1786942, Length=565, Percent_Identity=33.0973451327434, Blast_Score=221, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17505833, Length=530, Percent_Identity=32.8301886792453, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17550100, Length=534, Percent_Identity=31.2734082397004, Blast_Score=232, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322701, Length=533, Percent_Identity=33.0206378986867, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6322416, Length=534, Percent_Identity=33.3333333333333, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6320788, Length=487, Percent_Identity=28.7474332648871, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322511, Length=484, Percent_Identity=26.4462809917355, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137288, Length=527, Percent_Identity=32.6375711574953, Blast_Score=234, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24655642, Length=527, Percent_Identity=32.6375711574953, Blast_Score=234, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24655647, Length=527, Percent_Identity=32.6375711574953, Blast_Score=234, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24663005, Length=545, Percent_Identity=31.743119266055, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 61065; Mature: 60934

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPRLNIALISKAESIECNSRYAQGG
CCCCHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCC
IAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDIIKQLDQYKVQFKRESDGHF
EEEEECCHHHHHHHHCCCEECCCCEEHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCE
HLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQFFEHHVAVNLITQYHPHRTDN
EEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QGEVLGAYILDCQRNRIHTFLAKCVILATGGAGKTYRYTTNPMVATGDGVAMAYRAGARV
CCCCHHHHHHHCCCHHHHHHHHHHHHEEECCCCCEEEEECCCEEECCCCEEEHHHCCCCC
GNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKNPETGERFMKRYAPDQLELATRDVV
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHH
ARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQIYSTLLSIGIDMSQDLIPVVPAAHYQ
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
CGGVLTDVDGRTDLARLYAIGEVAFTGLHGANRLASNSLLEALVMGSNAARCTLKDIATP
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
LKLIDHIPSWSSPGEVNARRASQINAQWRGLRGEMTSYAGIVRTEAGLEDLLQLILKRKK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
IIEEYYWKHCITRDFIELRNIILNAELIVRAALSRRESRGGHFREDFPNKNFKAQESIAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHH
LGQADSSLG
HCCCCCCCC
>Mature Secondary Structure 
SDQLSQQGQSFEFDVLVIGTGLAGLHYCLQLLAIQPRLNIALISKAESIECNSRYAQGG
CCCHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCC
IAAAISPEDSLESHISDTVLAGDGLCYLPAVEFIIRQGPDIIKQLDQYKVQFKRESDGHF
EEEEECCHHHHHHHHCCCEECCCCEEHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCCE
HLAKEGGHSHRRIFNCGDQTGLTLTQTLNHLARQHQQIQFFEHHVAVNLITQYHPHRTDN
EEECCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QGEVLGAYILDCQRNRIHTFLAKCVILATGGAGKTYRYTTNPMVATGDGVAMAYRAGARV
CCCCHHHHHHHCCCHHHHHHHHHHHHEEECCCCCEEEEECCCEEECCCCEEEHHHCCCCC
GNMEFYQFHPTLLHHHTINNFLISEAVRGEGALLKNPETGERFMKRYAPDQLELATRDVV
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHH
ARAIFSEIEQGQAGYVHLDISHQSKSFLKKRFPQIYSTLLSIGIDMSQDLIPVVPAAHYQ
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
CGGVLTDVDGRTDLARLYAIGEVAFTGLHGANRLASNSLLEALVMGSNAARCTLKDIATP
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
LKLIDHIPSWSSPGEVNARRASQINAQWRGLRGEMTSYAGIVRTEAGLEDLLQLILKRKK
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
IIEEYYWKHCITRDFIELRNIILNAELIVRAALSRRESRGGHFREDFPNKNFKAQESIAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHH
LGQADSSLG
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]