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The map label for this gene is proS [H]

Identifier: 54293678

GI number: 54293678

Start: 826518

End: 828227

Strand: Reverse

Name: proS [H]

Synonym: lpl0731

Alternate gene names: 54293678

Gene position: 828227-826518 (Counterclockwise)

Preceding gene: 54293680

Following gene: 54293674

Centisome position: 24.76

GC content: 41.75

Gene sequence:

>1710_bases
ATGCGCGCATCTCAATGGTTTTTGGTCACTCAAAAAGAAACTCCCAACGATGCAGAAATAGCATCCCATCAATTAATGCT
AAGATCAGGAATGATTCGTAAGCTTGGCTCAGGACTTTATACCTGGATGCCACTTGGCCTTAGAGTGCTGCGTAAAGTTG
AAAATATAGTTCGGGAAGAAATGAACAAAACTCATGCCATGGAACTGCTTATGCCATCAGTACAACCTGCTGAATTATGG
CAAGAGACGGGACGTTGGGAAACCTTTGGTGGTCAATTATTAACTATGAAAGACAGTAATCAAAGAGAGTACTGCTTTGG
GCCTACCCATGAAGAAGTCATTACCGATATCATGCGTAATGAATTGCAATCGTATAAACAATTGCCAGTCAATTTTTATC
AAATACAAACCAAGTTTCGCGATGAAATCAGACCACGGTTTGGGGTAATGCGAGCCCGTGAGTTTATTATGAAAGATGCT
TACTCCTTCCACCTCAGTCTTGAAAGCCTGCAAGAAACCTATAAGGATATGTATCAAGCCTATTGCAGGATTTTTGATAG
AATGGGACTCAAATATCGAGCAGTTGAAGCTGATACGGGAGCCATTGGCGGTTCAGCGTCCCATGAGTTTCAGGTTTTGG
CTGAATCGGGTGAAGATCTGATTTTTTATAGTGACGCCAGCGACTATGCTGCAAATATTGAACAAGCGACCAGCTTAAAA
CCACCCAAAGCGAATCAATCATGCAATGAAACAATCACATTGGTCGATACTCCAAATAAAAAAACAATTGATGAAGTAGC
CAGCTTCCTTGGTATTGCCAGTAATCAAACCATCAAAACATTGATTGTCAAAGGCAAGGAACATCCTATGGTGGCTCTGG
TCTTAAGAGGAGACGATGAGCTCAATGAAGTCAAAGCAACCAAACATCCCTTAGTTCACTCTCCCCTGTCCTTTATTGAT
GAAGAGCTGATTTTAAAAACGCTAAAAACTCCCTTAGGCTCCATAGGACCAGTCAAATTAAACATTCCTGTCATTGTTGA
TCATCAAGCGTTGGCCATGCCTTCATTTGTTTGTGGTGCCAATCAGGCGGATAAACATTTTATCAATGCTGCATGGGAGC
GCGATGCAAAATACGATGATGCCTATGATTTACGCAACGTTAAAGAAGGTGATCCAAGCCCTGATGGAAGAGGAACGCTT
CACTGCTGTCGTGGAATAGAGGTAGGACACGTTTTCCAATTAGGTGATAAATACGCCAAAGCTATGAATGCGTCTGTTAT
CAATGAGCAAGGACAATTACAAACCATGATCATGGGCTGCTATGGGTTAGGGATCACTCGTGTAGTCGCTGCCGCGATTG
AGCAGCATCATGACGAACACGGAATCATCTGGCCTCAAGCTTTAGCACCTTTCCAAGTCAATATTATTCCTCTTAATGGC
GCGAGATCACACGCCGTTAAAGAACAGGCAGAGTCACTTTATCAGCAACTCAAATCTCATGGCATCGATGTATTACTCGA
TGATCGTAATGAAAGAGCAGGTGTATTATTTGCTGATAATGACTTGATAGGAATTCCTCACCGATTAGTGGTCAGTGAGA
GAAATCTGGAACAAGGCTGTATTGAGTACAAATCCCGAACTTCAAGCGAAACTCAACTGATCAATCTGGATAAGGTGGTT
AATTTTATTATTGAGTTAATTAATAAATAA

Upstream 100 bases:

>100_bases
GAACAGAGAAACGAAGAGCCTAAACTAAACATATCGCAAATTGAAGGGTGATAAGTATATAATATCGACCTTAATTATTT
ATAGAAATCCAGGAATAGAC

Downstream 100 bases:

>100_bases
GATTTTCTACCCCATTGACACTGCGTTATCTGCATTCCGGCAATGCTGTTCGGATTAAAAATAACCCGAACGGCATTAAA
GAGAAGCCAATAATACAGGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMPLGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELW
QETGRWETFGGQLLTMKDSNQREYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHLSLESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGEDLIFYSDASDYAANIEQATSLK
PPKANQSCNETITLVDTPNKKTIDEVASFLGIASNQTIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFID
EELILKTLKTPLGSIGPVKLNIPVIVDHQALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDPSPDGRGTL
HCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVAAAIEQHHDEHGIIWPQALAPFQVNIIPLNG
ARSHAVKEQAESLYQQLKSHGIDVLLDDRNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVV
NFIIELINK

Sequences:

>Translated_569_residues
MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMPLGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELW
QETGRWETFGGQLLTMKDSNQREYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHLSLESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGEDLIFYSDASDYAANIEQATSLK
PPKANQSCNETITLVDTPNKKTIDEVASFLGIASNQTIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFID
EELILKTLKTPLGSIGPVKLNIPVIVDHQALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDPSPDGRGTL
HCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVAAAIEQHHDEHGIIWPQALAPFQVNIIPLNG
ARSHAVKEQAESLYQQLKSHGIDVLLDDRNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVV
NFIIELINK
>Mature_569_residues
MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMPLGLRVLRKVENIVREEMNKTHAMELLMPSVQPAELW
QETGRWETFGGQLLTMKDSNQREYCFGPTHEEVITDIMRNELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDA
YSFHLSLESLQETYKDMYQAYCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGEDLIFYSDASDYAANIEQATSLK
PPKANQSCNETITLVDTPNKKTIDEVASFLGIASNQTIKTLIVKGKEHPMVALVLRGDDELNEVKATKHPLVHSPLSFID
EELILKTLKTPLGSIGPVKLNIPVIVDHQALAMPSFVCGANQADKHFINAAWERDAKYDDAYDLRNVKEGDPSPDGRGTL
HCCRGIEVGHVFQLGDKYAKAMNASVINEQGQLQTMIMGCYGLGITRVVAAAIEQHHDEHGIIWPQALAPFQVNIIPLNG
ARSHAVKEQAESLYQQLKSHGIDVLLDDRNERAGVLFADNDLIGIPHRLVVSERNLEQGCIEYKSRTSSETQLINLDKVV
NFIIELINK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=42.5438596491228, Blast_Score=213, Evalue=5e-55,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=60.5263157894737, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI1788013, Length=127, Percent_Identity=29.1338582677165, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115532348, Length=257, Percent_Identity=33.852140077821, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=29.5238095238095, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=555, Percent_Identity=29.3693693693694, Blast_Score=235, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24656200, Length=231, Percent_Identity=35.0649350649351, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 64133; Mature: 64133

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMPLGLRVLRKVENIVREE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQREYCFGPTHEEVITDIMRN
HHHHHHHHHHCCCCCHHHHHHHCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
ELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHLSLESLQETYKDMYQA
HHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
YCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGEDLIFYSDASDYAANIEQATSLK
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCC
PPKANQSCNETITLVDTPNKKTIDEVASFLGIASNQTIKTLIVKGKEHPMVALVLRGDDE
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCH
LNEVKATKHPLVHSPLSFIDEELILKTLKTPLGSIGPVKLNIPVIVDHQALAMPSFVCGA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCHHHHCCHHHCCC
NQADKHFINAAWERDAKYDDAYDLRNVKEGDPSPDGRGTLHCCRGIEVGHVFQLGDKYAK
CCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHH
AMNASVINEQGQLQTMIMGCYGLGITRVVAAAIEQHHDEHGIIWPQALAPFQVNIIPLNG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
ARSHAVKEQAESLYQQLKSHGIDVLLDDRNERAGVLFADNDLIGIPHRLVVSERNLEQGC
CHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCEECCCCEEEEECCCHHHHH
IEYKSRTSSETQLINLDKVVNFIIELINK
HHHHHCCCCCCEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MRASQWFLVTQKETPNDAEIASHQLMLRSGMIRKLGSGLYTWMPLGLRVLRKVENIVREE
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNKTHAMELLMPSVQPAELWQETGRWETFGGQLLTMKDSNQREYCFGPTHEEVITDIMRN
HHHHHHHHHHCCCCCHHHHHHHCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
ELQSYKQLPVNFYQIQTKFRDEIRPRFGVMRAREFIMKDAYSFHLSLESLQETYKDMYQA
HHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
YCRIFDRMGLKYRAVEADTGAIGGSASHEFQVLAESGEDLIFYSDASDYAANIEQATSLK
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCC
PPKANQSCNETITLVDTPNKKTIDEVASFLGIASNQTIKTLIVKGKEHPMVALVLRGDDE
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCH
LNEVKATKHPLVHSPLSFIDEELILKTLKTPLGSIGPVKLNIPVIVDHQALAMPSFVCGA
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCHHHHCCHHHCCC
NQADKHFINAAWERDAKYDDAYDLRNVKEGDPSPDGRGTLHCCRGIEVGHVFQLGDKYAK
CCHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHH
AMNASVINEQGQLQTMIMGCYGLGITRVVAAAIEQHHDEHGIIWPQALAPFQVNIIPLNG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
ARSHAVKEQAESLYQQLKSHGIDVLLDDRNERAGVLFADNDLIGIPHRLVVSERNLEQGC
CHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCEECCCCEEEEECCCHHHHH
IEYKSRTSSETQLINLDKVVNFIIELINK
HHHHHCCCCCCEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA