Click here to switch to the map view.

The map label for this gene is parE [H]

Identifier: 54293674

GI number: 54293674

Start: 816801

End: 818681

Strand: Reverse

Name: parE [H]

Synonym: lpl0727

Alternate gene names: 54293674

Gene position: 818681-816801 (Counterclockwise)

Preceding gene: 54293678

Following gene: 54293669

Centisome position: 24.47

GC content: 43.01

Gene sequence:

>1881_bases
ATGTCAGAAAACTACACTGCGGAAGCGATTGAAGTCTTAAGTGGTCTTGAGCCTGTTCAACGACGTCCCGGTATGTATAC
CGACACGACACGCCCCAACCATTTGGCTCAGGAAGTGATTGATAATAGCGTCGATGAAGTACTTGCTGGATTTGCCAGCC
GTATTATTGTTACTTTACATGAAGACGGCTCTATTGAAGTCGAGGATGATGGCCGAGGTATGCCAGTCGATTTACACCCT
CAACTCGGCTTAAGTGGTGTCGAGGTCATTATGACTCGACTTCATGCCGGAGGTAAGTTTTCAGATAAAAATTACAGCTT
TTCCGGCGGCTTGCATGGCGTTGGGGTATCAGTGGTAAATGCCTTGTCAGAACGACTTGACGTCACCATAAAGCGCAATG
GGATCATTTATCAAATGACCTTTGCTAATGGCGACAAATTATGTGAATTAAATGAAACCGGGGTTACAAAAAAAAGAGAA
ACCGGGACTGTTATACGTTTTTGGCCTAATGGCAAATATTTTGATACTACAAAAATTTCTGTAAAACACCTGACTCATGT
ATTAAGAGCAAAAGCGGTATTGTGTAGTGGCTTGTCCATGACCTTTATCAATAAAATCACTAATGAAGAAATGAACTGGT
GTTATGAACAAGGTTTAACTGATTATTTACGGCAAACCTTGCCCGAAGACTATTTGCCTGAAGAACCTTTTGCCGGAGCA
TTCAAAAGTGATGAAGCAACCGTTGATTGGGCTTTGGCCTGGTCAGACTCTGCCAATGGCAATTTAAATGAAAGTTATGT
GAATTTAATCCCGACAATACAAGGAGGCACGCACGTCAATGGATTGCGTTCGGGATTATTTGAAGCCATGTCTGAATTTT
GTGAGCTGAGAAACCTTCTTCCTCGTGGAGTAAAATTGACAGCGGATGATCTATGGGAATCATGTCAGTATGTTTTATCA
ATCAAAATGAAAGAGCCGCAATTTGCTGGGCAAACCAAAGAGCGCTTAAGCTCACGCCAAACCTCAGGACTAGTCAGTAA
TGTCATAAAAGACGCTTTTGCAATTTGGTTGAATCAGCATCGCAACCAGGGTGAAGCCATAGCAGCTCTTGCAATAGAAC
GCGCACAGAAACGGCTAAAGCAAGCCAAACAAGTGGCACGCAAACGTGTTAGCCAAGGGCCTGCTTTACCTGGAAAATTA
GCCGACTGCTTGCAAACGGACTTAGGTCAAGCGGAACTATTCCTGGTTGAAGGAGATTCGGCAGGAGGGTCGGCCAAACA
AGCCCGCAATAAAGATTATCAAGCCATTTTACCCTTACGTGGGAAAATACTTAATTCATGGGAAGTAGATTCTTCACAAG
TTCTGGCATCCCAGGAAATTCATGATATATCTGTTGCTATAGGCGTAGATCCTGGCTCTAGCGATCTTGAGGGTCTTCGC
TATGGAAAAATCTGTATTCTTGCCGATGCAGACTCTGATGGAGCTCATATTGCCACACTAATATGTGCCTTGTTTTTAAG
ACACTTCAAACCTCTGGTTAAAGCAGGCCATGTTTTTGTAGCCATGCCCCCGCTCTATCGAATTGATGCAGGTAAGCTGA
TTCACTATGCCCTGGATGATGAAGAAAAAAATAGAATCGTTCAACATCTCAGTCAAACCACAAAATCAAAAGTGAATGTA
CAGCGATTCAAAGGATTAGGTGAAATGAATCCCATTCAGCTTAGAGAAACAACAATGGATCCCAATACAAGGCGTCTGGT
ACAGCTTACTCTTGATGATGAAGAGCAGACGGAAGCCATTATGGATATGATGCTCAACAAAAAAAGGGCCTCCGACAGAA
AACTCTGGCTAGAAACAAAAGGAAACCTTGCTGAAATTTGA

Upstream 100 bases:

>100_bases
AACAAGAGAGGGGTGATAGTAGCAATAAAAATACCATACCACTCTGAGCAAAAGAATGTTTATGATAGAATAAGCACAAT
TAATATTTGAGTGAATGCCC

Downstream 100 bases:

>100_bases
GCTATGGATAGCCCGCATCACGATAAAGCAAGGATACAATTGAATTTGATAGCTTATCTTTTTACCCTACCATTTCAGTA
AGGGAATAATATTAGAATAA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSVDEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHP
QLGLSGVEVIMTRLHAGGKFSDKNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQGLTDYLRQTLPEDYLPEEPFAGA
FKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGGTHVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLS
IKMKEPQFAGQTKERLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQGPALPGKL
ADCLQTDLGQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVDSSQVLASQEIHDISVAIGVDPGSSDLEGLR
YGKICILADADSDGAHIATLICALFLRHFKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIVQHLSQTTKSKVNV
QRFKGLGEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRASDRKLWLETKGNLAEI

Sequences:

>Translated_626_residues
MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSVDEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHP
QLGLSGVEVIMTRLHAGGKFSDKNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRE
TGTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQGLTDYLRQTLPEDYLPEEPFAGA
FKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGGTHVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLS
IKMKEPQFAGQTKERLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQGPALPGKL
ADCLQTDLGQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVDSSQVLASQEIHDISVAIGVDPGSSDLEGLR
YGKICILADADSDGAHIATLICALFLRHFKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIVQHLSQTTKSKVNV
QRFKGLGEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRASDRKLWLETKGNLAEI
>Mature_625_residues
SENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSVDEVLAGFASRIIVTLHEDGSIEVEDDGRGMPVDLHPQ
LGLSGVEVIMTRLHAGGKFSDKNYSFSGGLHGVGVSVVNALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRET
GTVIRFWPNGKYFDTTKISVKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQGLTDYLRQTLPEDYLPEEPFAGAF
KSDEATVDWALAWSDSANGNLNESYVNLIPTIQGGTHVNGLRSGLFEAMSEFCELRNLLPRGVKLTADDLWESCQYVLSI
KMKEPQFAGQTKERLSSRQTSGLVSNVIKDAFAIWLNQHRNQGEAIAALAIERAQKRLKQAKQVARKRVSQGPALPGKLA
DCLQTDLGQAELFLVEGDSAGGSAKQARNKDYQAILPLRGKILNSWEVDSSQVLASQEIHDISVAIGVDPGSSDLEGLRY
GKICILADADSDGAHIATLICALFLRHFKPLVKAGHVFVAMPPLYRIDAGKLIHYALDDEEKNRIVQHLSQTTKSKVNVQ
RFKGLGEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAIMDMMLNKKRASDRKLWLETKGNLAEI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=580, Percent_Identity=25.1724137931034, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI19913406, Length=619, Percent_Identity=24.5557350565428, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1789408, Length=631, Percent_Identity=65.13470681458, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=554, Percent_Identity=40.072202166065, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=595, Percent_Identity=24.0336134453782, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=595, Percent_Identity=22.8571428571429, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=250, Percent_Identity=24.8, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=680, Percent_Identity=25.4411764705882, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI17136538, Length=584, Percent_Identity=24.8287671232877, Blast_Score=117, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.- [C]

Molecular weight: Translated: 69427; Mature: 69296

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSVDEVLAGFASRIIVTLH
CCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCEEEEEEE
EDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSDKNYSFSGGLHGVGVSVVN
CCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
ALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRETGTVIRFWPNGKYFDTTKIS
HHHHHHEEEEECCCEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCCCEECCHHHH
VKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQGLTDYLRQTLPEDYLPEEPFAGA
HHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGGTHVNGLRSGLFEAMSEFCELRNLL
CCCCCCEEEEEEEECCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
PRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKERLSSRQTSGLVSNVIKDAFAIWLNQH
CCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RNQGEAIAALAIERAQKRLKQAKQVARKRVSQGPALPGKLADCLQTDLGQAELFLVEGDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCC
AGGSAKQARNKDYQAILPLRGKILNSWEVDSSQVLASQEIHDISVAIGVDPGSSDLEGLR
CCCCHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCE
YGKICILADADSDGAHIATLICALFLRHFKPLVKAGHVFVAMPPLYRIDAGKLIHYALDD
ECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCEEEEECCC
EEKNRIVQHLSQTTKSKVNVQRFKGLGEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAI
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHH
MDMMLNKKRASDRKLWLETKGNLAEI
HHHHHHHCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SENYTAEAIEVLSGLEPVQRRPGMYTDTTRPNHLAQEVIDNSVDEVLAGFASRIIVTLH
CCCCHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCEEEEEEE
EDGSIEVEDDGRGMPVDLHPQLGLSGVEVIMTRLHAGGKFSDKNYSFSGGLHGVGVSVVN
CCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
ALSERLDVTIKRNGIIYQMTFANGDKLCELNETGVTKKRETGTVIRFWPNGKYFDTTKIS
HHHHHHEEEEECCCEEEEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCCCEECCHHHH
VKHLTHVLRAKAVLCSGLSMTFINKITNEEMNWCYEQGLTDYLRQTLPEDYLPEEPFAGA
HHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FKSDEATVDWALAWSDSANGNLNESYVNLIPTIQGGTHVNGLRSGLFEAMSEFCELRNLL
CCCCCCEEEEEEEECCCCCCCCCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
PRGVKLTADDLWESCQYVLSIKMKEPQFAGQTKERLSSRQTSGLVSNVIKDAFAIWLNQH
CCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RNQGEAIAALAIERAQKRLKQAKQVARKRVSQGPALPGKLADCLQTDLGQAELFLVEGDS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCC
AGGSAKQARNKDYQAILPLRGKILNSWEVDSSQVLASQEIHDISVAIGVDPGSSDLEGLR
CCCCHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCE
YGKICILADADSDGAHIATLICALFLRHFKPLVKAGHVFVAMPPLYRIDAGKLIHYALDD
ECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCEEEEECCC
EEKNRIVQHLSQTTKSKVNVQRFKGLGEMNPIQLRETTMDPNTRRLVQLTLDDEEQTEAI
HHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHH
MDMMLNKKRASDRKLWLETKGNLAEI
HHHHHHHCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]