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The map label for this gene is oprM [H]

Identifier: 54292982

GI number: 54292982

Start: 22017

End: 23402

Strand: Direct

Name: oprM [H]

Synonym: lpl0018

Alternate gene names: 54292982

Gene position: 22017-23402 (Clockwise)

Preceding gene: 54292981

Following gene: 54292984

Centisome position: 0.66

GC content: 36.58

Gene sequence:

>1386_bases
ATGCTACGTCCGGTTAAAATCATTTTATTATCAGTCTTTTTAGTCGGATGCTATAGTGAGCCTTATCACAGACCCCATGT
TGAAGTAATTAATAAATGGTCGGTAAAAGACAAAAATGTTAAAAATATCGATAGCAAGAATATTCCTTACATAGCCTGGT
GGCGTGATTTTAAAGACCCCACTCTCAACCAGTTGATTGAAAGAGGATTGATTTCCAATACAAGCCTTGGCATGTCAAGA
GGGAGCATTGAAGCGGCAGAGGGTGAGCTTAAAAAAATTCGTTATCAGTGGGTGCCTACCCTCGATCTTATGACTGGTTA
TTCAAGAAATCCGGCAACGGGTTTTCCTGGCGTTCTGATTGTTTTTATTCCTAACTATACAATAAACATCATCAATCAAA
TAAGAGAGCAAAAAAGGGCAAAATATACTCTTGCACAGGTAAAAGCAGAGGACGATGCTCTCAAACTAACAATTATTTCT
CAAATAACGGCAAGTTATTTCACCTATCTTGCAGAAATAGAACGCAAAGAGTTTTTACAGACATTAGCCAATGATTTAAC
TCAGTTGGCAAAGGTTGCCAGTAAAGTTTACCAAGGAGGATTAAGTTCTGAAATTGAGCAGGAAGAGTTGTATAGCCAGG
TCAATTTGATACAAGGCGAACTCGAGGTAACAGAGCGTAATATCGTTATAAGTCGCAATGCTCTCCGGTATTTACTAAAC
CAAAATCCAGGGGAAATAAAAACAGTTAAAAAATTATTACAACTTAAAAATCGTAAATTAATACCCGGTTCTTTACCGTT
AACAGTTCTTGAAAATCGGCCTGATATGCAAGTAGCTGAAAATCGTTTGCGTGCTTCACATGAAGGAATTGGTGTTGCAG
AAAGTAATCTTTTACCTACTATACAATTAGATTATATTGGTGGTTTGGCAGCTGGAGATAGCCGCTACGCATTACCGAAA
AAAACGGTGAAATTTAATGATCAATTGTTGAAAGCCCCCATTATTGATATGCCTGTTTTAGGTGCAGTAGCTAAGGCTAA
AGGCGTAAATAAGGTTTCTTATTATAATTATATTGATACTTTACAAAAAGCATTAAGAGATACGACCAATGCCTTATCAG
AAAATGAGCGTTTAACCAATAAGCTCAAACAAACTGCGTCGGCTCAAAAACATTTGGCAAAAGCATATAATTTGAACGAC
AGGTTATATCAGACAGGTATTCAAAGTTATATAGAAACCTTAAATAGCAAAATTTCGCTCGATAAAATCAACATCAGTTT
AAATCAGGATAAATTGCAAGAGCTACTAAGCATTGTACGTTTATATCAAGAATTAGCAGGTGGATATCGTGCTAAACCTC
AGTCACCCATAAGCCCTTCATCTTGA

Upstream 100 bases:

>100_bases
GATTATTATCAACTATTTCAACAGGTTCAAAAAGATTTGGTGCACTTAAAAGATTTTGTTGCAGGGGAGCAAACAATTGA
TCTTAGTGAGGGAGTAAAGC

Downstream 100 bases:

>100_bases
TTTTCATAACTTCTTATCAATACTCTGGCCCTACTAATTTAGATTAATAGTGGTTGGAGTTAAAGTGGGCAAGGGTTTTT
CTTCTACTGTAGGTCCTGGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSR
GSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
QITASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPK
KTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLND
RLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRAKPQSPISPSS

Sequences:

>Translated_461_residues
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSR
GSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
QITASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPK
KTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLND
RLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRAKPQSPISPSS
>Mature_461_residues
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDPTLNQLIERGLISNTSLGMSR
GSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLIVFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIIS
QITASYFTYLAEIERKEFLQTLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPTIQLDYIGGLAAGDSRYALPK
KTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDTLQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLND
RLYQTGIQSYIETLNSKISLDKINISLNQDKLQELLSIVRLYQELAGGYRAKPQSPISPSS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=497, Percent_Identity=23.5412474849095, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367348, Length=435, Percent_Identity=19.0804597701149, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51991; Mature: 51991

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDP
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCC
TLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLI
HHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEE
VFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIISQITASYFTYLAEIERKEFLQ
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
TLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHEEEECCEEEEHHHHHHHHC
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPT
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
IQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDT
EEEECCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLNDRLYQTGIQSYIETLNSKISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
DKINISLNQDKLQELLSIVRLYQELAGGYRAKPQSPISPSS
EEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRPVKIILLSVFLVGCYSEPYHRPHVEVINKWSVKDKNVKNIDSKNIPYIAWWRDFKDP
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCC
TLNQLIERGLISNTSLGMSRGSIEAAEGELKKIRYQWVPTLDLMTGYSRNPATGFPGVLI
HHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCEEE
VFIPNYTINIINQIREQKRAKYTLAQVKAEDDALKLTIISQITASYFTYLAEIERKEFLQ
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
TLANDLTQLAKVASKVYQGGLSSEIEQEELYSQVNLIQGELEVTERNIVISRNALRYLLN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHEEEECCEEEEHHHHHHHHC
QNPGEIKTVKKLLQLKNRKLIPGSLPLTVLENRPDMQVAENRLRASHEGIGVAESNLLPT
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCE
IQLDYIGGLAAGDSRYALPKKTVKFNDQLLKAPIIDMPVLGAVAKAKGVNKVSYYNYIDT
EEEECCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LQKALRDTTNALSENERLTNKLKQTASAQKHLAKAYNLNDRLYQTGIQSYIETLNSKISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
DKINISLNQDKLQELLSIVRLYQELAGGYRAKPQSPISPSS
EEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]