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The map label for this gene is recF [H]

Identifier: 54292967

GI number: 54292967

Start: 2676

End: 3737

Strand: Direct

Name: recF [H]

Synonym: lpl0003

Alternate gene names: 54292967

Gene position: 2676-3737 (Clockwise)

Preceding gene: 54292966

Following gene: 54292968

Centisome position: 0.08

GC content: 35.4

Gene sequence:

>1062_bases
ATGATTTTATCTGAAGTTAGAATTCATAATTTTCGGAATATCGCTTCGACTAGTTTAATCCTAAATCCCAATTTTAATTG
TATTACAGGACCTAATGGCAGTGGTAAGACCTCGTTACTAGAGGCTCTTTACATGTTGAGTTGCGCCCATTCTTTTCGGT
CTCGTGAGGTAACTCCAATTATTTCTTATGGTCAAAACCAACTTAATGTTTTTGCCCATGCTTATGACGAAAGCACTATT
AGTGTTCAGAAATCTATAACAGATGGCACACAAATTAAATTGAATAACCAGTTTTGCTGTACTACAAGTCAACTAGCCTA
TGCGCTTCCTTGCCAGGTTATTTACTCAGATATTTTTCAAATTATTGATGCCGGCCCCTCGGTAAGGAGAAGCTTGCTTG
ATTGGGGGTTGTTTCACGTGAAACATGATTATTTGAAAATTTGGAAGGATTACAAGCGCATACTTAGCCAACGAAATGCA
TTATTAAAATCAAGAGCTACTTATGAACATTTTATTCCTTGGGACCAACAGCTAAGTCAATTGGCAAATCAACTGGATAA
AGCTAGAAATGACTATTTTTTGCAATGGCAACCTAAATTCTACCAAGTCTTAAGTGATTTAACTGATATATCCTGTACAG
TTGAGTATTACAAAGGGTGGGATAGAAAAAATGCAGGGCAGAACATGGAGGAACTGCTTCAAAAATCATTTGATAGTGAT
AGAAATAAACTATATACGCAATATGGACCTCATCAAGCTGATTTGATAATTAGCACTGAACAATACAGAGTAAAGCATAC
ACTCTCTCGTGGACAACAAAAAATAATTCTAATCGCGCTTAAACTGGCTCAAGGTCAGCTATTAGATAAAGATTGCTTAT
ACCTTATTGATGATTTGGCTGCTGAATTAGATGACTATCACCAGCGAAATTTAATTAAGTATCTGGCACAACTAAAGGGG
CAGTTTGTTATAACGAATTTAATTAACAACAATAATTACGATATCCTGCCAATTGATTCAGGTTTGTTTGAAGTAAACTG
TGGCGCAATTAAAGTTTTATAA

Upstream 100 bases:

>100_bases
AAGGTCAAATCAGGTTATCAATGTCTAATACGGAAAGCAGTATTTTAATTGAGTCATTAATCAATGATAATTATCAATAC
ATTATTATGCCTATGAAAAT

Downstream 100 bases:

>100_bases
TATAGAATGTTTCACGTGGAACACAAATTAATGGAATGAAAAGTTCCATTATTAAACAAAATCACCTTAATGCAAATAGA
ATACAAAATTCTAAAGATAA

Product: RecF recombinational DNA repair ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVTPIISYGQNQLNVFAHAYDESTI
SVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTDISCTVEYYKGWDRKNAGQNMEELLQKSFDSD
RNKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLAQLKG
QFVITNLINNNNYDILPIDSGLFEVNCGAIKVL

Sequences:

>Translated_353_residues
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVTPIISYGQNQLNVFAHAYDESTI
SVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTDISCTVEYYKGWDRKNAGQNMEELLQKSFDSD
RNKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLAQLKG
QFVITNLINNNNYDILPIDSGLFEVNCGAIKVL
>Mature_353_residues
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVTPIISYGQNQLNVFAHAYDESTI
SVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTDISCTVEYYKGWDRKNAGQNMEELLQKSFDSD
RNKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLAQLKG
QFVITNLINNNNYDILPIDSGLFEVNCGAIKVL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=358, Percent_Identity=34.6368715083799, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40654; Mature: 40654

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVTPI
CCCCHHHHHHHHHHHCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
ISYGQNQLNVFAHAYDESTISVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQ
HHCCCCCEEEEEEECCCCCEEEEHHCCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHH
IIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNALLKSRATYEHFIPWDQQLSQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHH
LANQLDKARNDYFLQWQPKFYQVLSDLTDISCTVEYYKGWDRKNAGQNMEELLQKSFDSD
HHHHHHHCCCCCEEEECHHHHHHHHHHHHHEEEEHHHCCCCCCCCCCCHHHHHHHHCCCH
RNKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLA
HHEEECCCCCCCEEEEEECHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AELDDYHQRNLIKYLAQLKGQFVITNLINNNNYDILPIDSGLFEVNCGAIKVL
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCEEEECCCEEEEC
>Mature Secondary Structure
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVTPI
CCCCHHHHHHHHHHHCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
ISYGQNQLNVFAHAYDESTISVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQ
HHCCCCCEEEEEEECCCCCEEEEHHCCCCCEEEECCEEEECHHHHHHHHHHHHHHHHHHH
IIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNALLKSRATYEHFIPWDQQLSQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHH
LANQLDKARNDYFLQWQPKFYQVLSDLTDISCTVEYYKGWDRKNAGQNMEELLQKSFDSD
HHHHHHHCCCCCEEEECHHHHHHHHHHHHHEEEEHHHCCCCCCCCCCCHHHHHHHHCCCH
RNKLYTQYGPHQADLIISTEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLA
HHEEECCCCCCCEEEEEECHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
AELDDYHQRNLIKYLAQLKGQFVITNLINNNNYDILPIDSGLFEVNCGAIKVL
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA