Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 54027630

GI number: 54027630

Start: 5995441

End: 5996898

Strand: Reverse

Name: cca [H]

Synonym: nfa56560

Alternate gene names: 54027630

Gene position: 5996898-5995441 (Counterclockwise)

Preceding gene: 54027639

Following gene: 54027628

Centisome position: 99.6

GC content: 70.03

Gene sequence:

>1458_bases
GTGTCCGATACAGCTCCGCTTCCCTCCGAGGATCGGCGTACGCGGTTGCTTGCTTCGGCGGCGGTGACCCTCGGGGCGCT
TGCCGACGTGCTCACCCCGCTCGGTGGGTTGTTCGCGGCGCGCGGGCACGAGTTGTACCTGGTGGGCGGCAGTGTGCGGG
ACGCGGTGCTCGGACGGTTGGGCACCGATCTGGATTTCACCACCGACGCGCGGCCGGAGCAGGTGCTCGAGATCATGCGC
GGGTGGGCCGACCATGTATGGGACACCGGGGGCCTGGCCTTCGGCACGGTGAGCGCCGCCAAGGCGGGGCAGCAGCTCGA
GATCACCACCTTCCGCAGCGACACCTACGACCGGGTCTCCCGGAACCCGGAGGTGACCTTCGGCGACACGCTCGAGGGTG
ATCTGGTGCGCCGGGACTTCACCGTCAACGCGATGGCGGTGCGCATCGCGGCCGACGGTTCGCTGGAGTTCGTCGACCCG
CTCGGCGGAATGGACGCCCTGCTGGCGGGGGTGCTGGACACCCCGGCCTCGCCGGAGGACTCCTTCGACGACGATCCGCT
GCGGATGCTGCGGGCGGCGCGGTTCGTCTCGCAACTCGGCTTCGAGCTCGCCCCGCGGGTGCGGGCGGCGATCGGCCGGA
TGGCCGAGCAGATCGACCGGATCACCGCCGAGCGGGTGCGGGTCGAACTCGACAAGCTCATCGCCGGTGACCACCCGATC
GACGGCATCGACATCATGTGCGAGACGGGCCTGGCCCAGCGGGTGCTGCCGGAGGTGCCCGCCATGAAGCTCGAGATCGA
CGAGCACCACCAGCACAAGGATGTGTACCGGCATTCGCTGACCGTGCTCGAGCAGGCCATCGACCTCGAGGACGGCGACC
CCGACCTGGTGCTGCGCTGGGCGGCCCTGCTGCACGACATCGGCAAGCCCGACACCAAGCGCAACGAACCAGGCGGTGGC
GTCAGCTTCCACCACCACGAGGTGGTCGGCGCGAAGATGGTGCGCAAACGGATGCGCGCGCTGAAGTACCCGAAACAGTT
CACCGAGGACGTGGCCCGCCTGGTGTTCCTACACCTGCGCTTCCACGGCTACGGCAAGGGCCAGTGGACCGATTCGGCGG
TGCGGCGCTACGTCACCGACGCCGGTGACCTGCTGCCCCGCCTGCACAAGCTGGTGCGCGCCGACTGCACCACCCGCAAC
AAGCGCCGCGCCAGTGCGCTGCAGGCCACCTACGACGAGCTGGAGGAGCGCATCGCGCGGCTCCAGCAGGAGGAGGACCT
GGCCAAGGTCCGGCCCGACCTGGACGGCAACGCCATCATGGAGCTGCTCGGCCTGCCGCCGGGGCCCGAGGTCGGCCGCG
CCTGGCGGTACCTGAAGGAACTGCGCTTGGACCGCGGTCCGCTCACCCGCGAGGAGGCCGAGGCCGCCCTGGTGGAGTGG
TGGAAGAGCCAGAGCTGA

Upstream 100 bases:

>100_bases
TGTGTCTCCCGGGTGTGGGGCCGTGGGGACGGGGTGTGGTGGGGGGTCGGCCTCTATATACAGTTCCGTTGTGGTTTCGC
CCGAGTCCGAGGATGTTGTC

Downstream 100 bases:

>100_bases
CGCTCAGAAGACGATCAGCGTGGTGCCCGCCACCACGGCGTCGCGGATCTGCGCGAGGTCGAACGAGGCCGTCGTCTCCG
GCAGCTCCAGCTCGAACCGC

Product: putative tRNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSDTAPLPSEDRRTRLLASAAVTLGALADVLTPLGGLFAARGHELYLVGGSVRDAVLGRLGTDLDFTTDARPEQVLEIMR
GWADHVWDTGGLAFGTVSAAKAGQQLEITTFRSDTYDRVSRNPEVTFGDTLEGDLVRRDFTVNAMAVRIAADGSLEFVDP
LGGMDALLAGVLDTPASPEDSFDDDPLRMLRAARFVSQLGFELAPRVRAAIGRMAEQIDRITAERVRVELDKLIAGDHPI
DGIDIMCETGLAQRVLPEVPAMKLEIDEHHQHKDVYRHSLTVLEQAIDLEDGDPDLVLRWAALLHDIGKPDTKRNEPGGG
VSFHHHEVVGAKMVRKRMRALKYPKQFTEDVARLVFLHLRFHGYGKGQWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRN
KRRASALQATYDELEERIARLQQEEDLAKVRPDLDGNAIMELLGLPPGPEVGRAWRYLKELRLDRGPLTREEAEAALVEW
WKSQS

Sequences:

>Translated_485_residues
MSDTAPLPSEDRRTRLLASAAVTLGALADVLTPLGGLFAARGHELYLVGGSVRDAVLGRLGTDLDFTTDARPEQVLEIMR
GWADHVWDTGGLAFGTVSAAKAGQQLEITTFRSDTYDRVSRNPEVTFGDTLEGDLVRRDFTVNAMAVRIAADGSLEFVDP
LGGMDALLAGVLDTPASPEDSFDDDPLRMLRAARFVSQLGFELAPRVRAAIGRMAEQIDRITAERVRVELDKLIAGDHPI
DGIDIMCETGLAQRVLPEVPAMKLEIDEHHQHKDVYRHSLTVLEQAIDLEDGDPDLVLRWAALLHDIGKPDTKRNEPGGG
VSFHHHEVVGAKMVRKRMRALKYPKQFTEDVARLVFLHLRFHGYGKGQWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRN
KRRASALQATYDELEERIARLQQEEDLAKVRPDLDGNAIMELLGLPPGPEVGRAWRYLKELRLDRGPLTREEAEAALVEW
WKSQS
>Mature_484_residues
SDTAPLPSEDRRTRLLASAAVTLGALADVLTPLGGLFAARGHELYLVGGSVRDAVLGRLGTDLDFTTDARPEQVLEIMRG
WADHVWDTGGLAFGTVSAAKAGQQLEITTFRSDTYDRVSRNPEVTFGDTLEGDLVRRDFTVNAMAVRIAADGSLEFVDPL
GGMDALLAGVLDTPASPEDSFDDDPLRMLRAARFVSQLGFELAPRVRAAIGRMAEQIDRITAERVRVELDKLIAGDHPID
GIDIMCETGLAQRVLPEVPAMKLEIDEHHQHKDVYRHSLTVLEQAIDLEDGDPDLVLRWAALLHDIGKPDTKRNEPGGGV
SFHHHEVVGAKMVRKRMRALKYPKQFTEDVARLVFLHLRFHGYGKGQWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRNK
RRASALQATYDELEERIARLQQEEDLAKVRPDLDGNAIMELLGLPPGPEVGRAWRYLKELRLDRGPLTREEAEAALVEWW
KSQS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=275, Percent_Identity=30.1818181818182, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=32.1739130434783, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789436, Length=320, Percent_Identity=30.9375, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=230, Percent_Identity=27.3913043478261, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=246, Percent_Identity=28.8617886178862, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281360112, Length=228, Percent_Identity=28.5087719298246, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357337, Length=228, Percent_Identity=28.5087719298246, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53932; Mature: 53801

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTAPLPSEDRRTRLLASAAVTLGALADVLTPLGGLFAARGHELYLVGGSVRDAVLGRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
GTDLDFTTDARPEQVLEIMRGWADHVWDTGGLAFGTVSAAKAGQQLEITTFRSDTYDRVS
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHCCCEEEEEEECCCCHHHHC
RNPEVTFGDTLEGDLVRRDFTVNAMAVRIAADGSLEFVDPLGGMDALLAGVLDTPASPED
CCCCEEECCCCCCCHHHCCEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCC
SFDDDPLRMLRAARFVSQLGFELAPRVRAAIGRMAEQIDRITAERVRVELDKLIAGDHPI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DGIDIMCETGLAQRVLPEVPAMKLEIDEHHQHKDVYRHSLTVLEQAIDLEDGDPDLVLRW
CCHHHHCCCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AALLHDIGKPDTKRNEPGGGVSFHHHEVVGAKMVRKRMRALKYPKQFTEDVARLVFLHLR
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FHGYGKGQWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRNKRRASALQATYDELEERIAR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LQQEEDLAKVRPDLDGNAIMELLGLPPGPEVGRAWRYLKELRLDRGPLTREEAEAALVEW
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
WKSQS
HHCCC
>Mature Secondary Structure 
SDTAPLPSEDRRTRLLASAAVTLGALADVLTPLGGLFAARGHELYLVGGSVRDAVLGRL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
GTDLDFTTDARPEQVLEIMRGWADHVWDTGGLAFGTVSAAKAGQQLEITTFRSDTYDRVS
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHCCCEEEEEEECCCCHHHHC
RNPEVTFGDTLEGDLVRRDFTVNAMAVRIAADGSLEFVDPLGGMDALLAGVLDTPASPED
CCCCEEECCCCCCCHHHCCEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCC
SFDDDPLRMLRAARFVSQLGFELAPRVRAAIGRMAEQIDRITAERVRVELDKLIAGDHPI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DGIDIMCETGLAQRVLPEVPAMKLEIDEHHQHKDVYRHSLTVLEQAIDLEDGDPDLVLRW
CCHHHHCCCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AALLHDIGKPDTKRNEPGGGVSFHHHEVVGAKMVRKRMRALKYPKQFTEDVARLVFLHLR
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FHGYGKGQWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRNKRRASALQATYDELEERIAR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LQQEEDLAKVRPDLDGNAIMELLGLPPGPEVGRAWRYLKELRLDRGPLTREEAEAALVEW
HHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
WKSQS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA