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The map label for this gene is tcmA [H]

Identifier: 54027609

GI number: 54027609

Start: 5975924

End: 5977474

Strand: Reverse

Name: tcmA [H]

Synonym: nfa56350

Alternate gene names: 54027609

Gene position: 5977474-5975924 (Counterclockwise)

Preceding gene: 54027610

Following gene: 54027607

Centisome position: 99.27

GC content: 72.79

Gene sequence:

>1551_bases
GTGCGGCCGCGCCGGTCCGGTTACCGTGGACCGGTGGGTACCCCGGCGGAGCTTCCGCGGTTGCACTCGGCCACCGGCCG
CTGGATCCTGCTCGCCACCATTCTGGGGTCCTCGGTGGCCTCGCTCGACGCCACCGTCGTCAACATCGCGCTGCCTCGCA
TCGGCGAATCCCTCGACACCGATGTGGCGGGCCTGCAATGGACGCTCAACGGCTACACCCTCACCCTCGCCTCGTTCATC
CTGCTCGGCGGCTCGCTGGGTGACCGGCTCGGCCGCCGCCGGGTGTTCGTCTGGGGCACCGTGGGATTCGCGGTCACCTC
GGTGCTGTGCGGACTCGCGGTGAACATCGAGATGCTGGTGGCGGCCCGCGTGCTGCAGGGTGTCGCGGGTGCCATGCTCA
CCCCGGGCAGCCTGGCGCTGATCTCCTCCTCGCTCGACGAGCGCGACCAGGGCGCGGCGATCGGCCTGTGGTCCGGCTTC
GGCGGGGTGGCGGGTGCCCTCGGGCCGTTCCTCGGCGGCTGGCTGATCGACTGGGCGGGCTGGCAGTCCATCTTCTTCAT
CAACGTGCCGCTGGCGCTGATCGTGGTCGTGGTGGCGCTGAAACATGTGCCGGAGAGCCTGGATCCGCGGGCGCGCGGCA
AGCTGGACGTGCCCGGTGCGCTGATCGTGGCCGCCGCGCTCGGCGCGCTCACCTTCGGCCTGATCGATGGCCTGCCGCTG
TTGACGGTGGCGGGTGTGCTGCTGCTCGCGGTCTTCGTCGTGGTCGAACGGCGCAGCGACCACCCGCTGGTGCCGCCCGC
CCTGTTCACCGGTGCCTTCCCCGCCGACCGGCCCGGCGCCGCCGCCGCGAGCGGGCGGGTGTTCACCGCCGCCAATCTGG
TGACGCTGGCCGTCTATGCCGCGCTCGGCGGCGTGTTCTTCCTGCTGGTGCTGGAATTGCAGCTGGTGGCCGGCTTCTCG
CCGATGGCATCGGGTCTGGCGACGGTGCCCATCACGCTGATCATGCTGGTGCTCTCCGCGCCCGCGGGCCGCTGGGCGCA
GCGACACGGCGCCCGGGTTCCGATGACCGTCGGCCCGCTGCTCGGTGCCCTGGGCCTGGTGCTGCTGCTGCGCATCGGCG
AGGAGGCGACCTACCTCACCGATGTACTGCCCGGCGTGCTGGTGTTCGGTCTCGGCCTCGCCGCCCTGGTCGCGCCGTTG
ACCGGCGCGGTCCTCGGTGCGGTCCCGCCGACCGAGGCCGGCGTCGCGTCCGGGGTGAACAACGCGGTCGCGCGGACCGC
GCAGTTGCTCGCCGTCGCGGCCCTGCCCGGCCTGGCGGGGATCTCCGGTTCGCTCACCGACCCGGCGGCCTTCGACGCCG
GTTTCGAGACCGCGATGTGGATCTGCGTGGGCCTGCTGGTGGTGGGCGCCCTGCTGGCCGCGGTGTTGCTGCGGCCGGTG
CGGCGGGCGCCGCGGCTGGACGAGGTGGACTGCCTGCCGCAGTGCGGGGTCGCCGGCCCGGCACTGTCGCCGACGCGCCC
GAGTGTCGTGGGTGAATCACGCGAGAAGTGA

Upstream 100 bases:

>100_bases
AGGCGCGTGCGGCTCCGGGCTGGGTTTCGAGTACCCGGAGCGCCGGTTCGTGGTCTGCGTCGAGGAGCTGTAGCGCGCGC
GTCGACCCGTCGTTGTCCCG

Downstream 100 bases:

>100_bases
CTCGACAAAAGAAATAAGGAGCGGGCCGTTCGCTGCCCGCTCCTCGTCTTCCGCGCCCGTCGATCAGATCGGGACGTACC
CCGAGCCCACTCCGCCGCGC

Product: putative transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MRPRRSGYRGPVGTPAELPRLHSATGRWILLATILGSSVASLDATVVNIALPRIGESLDTDVAGLQWTLNGYTLTLASFI
LLGGSLGDRLGRRRVFVWGTVGFAVTSVLCGLAVNIEMLVAARVLQGVAGAMLTPGSLALISSSLDERDQGAAIGLWSGF
GGVAGALGPFLGGWLIDWAGWQSIFFINVPLALIVVVVALKHVPESLDPRARGKLDVPGALIVAAALGALTFGLIDGLPL
LTVAGVLLLAVFVVVERRSDHPLVPPALFTGAFPADRPGAAAASGRVFTAANLVTLAVYAALGGVFFLLVLELQLVAGFS
PMASGLATVPITLIMLVLSAPAGRWAQRHGARVPMTVGPLLGALGLVLLLRIGEEATYLTDVLPGVLVFGLGLAALVAPL
TGAVLGAVPPTEAGVASGVNNAVARTAQLLAVAALPGLAGISGSLTDPAAFDAGFETAMWICVGLLVVGALLAAVLLRPV
RRAPRLDEVDCLPQCGVAGPALSPTRPSVVGESREK

Sequences:

>Translated_516_residues
MRPRRSGYRGPVGTPAELPRLHSATGRWILLATILGSSVASLDATVVNIALPRIGESLDTDVAGLQWTLNGYTLTLASFI
LLGGSLGDRLGRRRVFVWGTVGFAVTSVLCGLAVNIEMLVAARVLQGVAGAMLTPGSLALISSSLDERDQGAAIGLWSGF
GGVAGALGPFLGGWLIDWAGWQSIFFINVPLALIVVVVALKHVPESLDPRARGKLDVPGALIVAAALGALTFGLIDGLPL
LTVAGVLLLAVFVVVERRSDHPLVPPALFTGAFPADRPGAAAASGRVFTAANLVTLAVYAALGGVFFLLVLELQLVAGFS
PMASGLATVPITLIMLVLSAPAGRWAQRHGARVPMTVGPLLGALGLVLLLRIGEEATYLTDVLPGVLVFGLGLAALVAPL
TGAVLGAVPPTEAGVASGVNNAVARTAQLLAVAALPGLAGISGSLTDPAAFDAGFETAMWICVGLLVVGALLAAVLLRPV
RRAPRLDEVDCLPQCGVAGPALSPTRPSVVGESREK
>Mature_516_residues
MRPRRSGYRGPVGTPAELPRLHSATGRWILLATILGSSVASLDATVVNIALPRIGESLDTDVAGLQWTLNGYTLTLASFI
LLGGSLGDRLGRRRVFVWGTVGFAVTSVLCGLAVNIEMLVAARVLQGVAGAMLTPGSLALISSSLDERDQGAAIGLWSGF
GGVAGALGPFLGGWLIDWAGWQSIFFINVPLALIVVVVALKHVPESLDPRARGKLDVPGALIVAAALGALTFGLIDGLPL
LTVAGVLLLAVFVVVERRSDHPLVPPALFTGAFPADRPGAAAASGRVFTAANLVTLAVYAALGGVFFLLVLELQLVAGFS
PMASGLATVPITLIMLVLSAPAGRWAQRHGARVPMTVGPLLGALGLVLLLRIGEEATYLTDVLPGVLVFGLGLAALVAPL
TGAVLGAVPPTEAGVASGVNNAVARTAQLLAVAALPGLAGISGSLTDPAAFDAGFETAMWICVGLLVVGALLAAVLLRPV
RRAPRLDEVDCLPQCGVAGPALSPTRPSVVGESREK

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=397, Percent_Identity=28.7153652392947, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081983, Length=400, Percent_Identity=28.25, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1790195, Length=327, Percent_Identity=30.8868501529052, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1788710, Length=403, Percent_Identity=25.8064516129032, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788392, Length=318, Percent_Identity=31.1320754716981, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790146, Length=170, Percent_Identity=28.8235294117647, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=273, Percent_Identity=27.8388278388278, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319770, Length=341, Percent_Identity=27.5659824046921, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=466, Percent_Identity=23.175965665236, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=26.1744966442953, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52672; Mature: 52672

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRRSGYRGPVGTPAELPRLHSATGRWILLATILGSSVASLDATVVNIALPRIGESLDT
CCCCCCCCCCCCCCCHHCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
DVAGLQWTLNGYTLTLASFILLGGSLGDRLGRRRVFVWGTVGFAVTSVLCGLAVNIEMLV
CCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
AARVLQGVAGAMLTPGSLALISSSLDERDQGAAIGLWSGFGGVAGALGPFLGGWLIDWAG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCC
WQSIFFINVPLALIVVVVALKHVPESLDPRARGKLDVPGALIVAAALGALTFGLIDGLPL
CCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
LTVAGVLLLAVFVVVERRSDHPLVPPALFTGAFPADRPGAAAASGRVFTAANLVTLAVYA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
ALGGVFFLLVLELQLVAGFSPMASGLATVPITLIMLVLSAPAGRWAQRHGARVPMTVGPL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHH
LGALGLVLLLRIGEEATYLTDVLPGVLVFGLGLAALVAPLTGAVLGAVPPTEAGVASGVN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHH
NAVARTAQLLAVAALPGLAGISGSLTDPAAFDAGFETAMWICVGLLVVGALLAAVLLRPV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAPRLDEVDCLPQCGVAGPALSPTRPSVVGESREK
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPRRSGYRGPVGTPAELPRLHSATGRWILLATILGSSVASLDATVVNIALPRIGESLDT
CCCCCCCCCCCCCCCHHCCHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
DVAGLQWTLNGYTLTLASFILLGGSLGDRLGRRRVFVWGTVGFAVTSVLCGLAVNIEMLV
CCCCEEEEECCHHHHHHHHHHHCCHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
AARVLQGVAGAMLTPGSLALISSSLDERDQGAAIGLWSGFGGVAGALGPFLGGWLIDWAG
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCC
WQSIFFINVPLALIVVVVALKHVPESLDPRARGKLDVPGALIVAAALGALTFGLIDGLPL
CCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
LTVAGVLLLAVFVVVERRSDHPLVPPALFTGAFPADRPGAAAASGRVFTAANLVTLAVYA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
ALGGVFFLLVLELQLVAGFSPMASGLATVPITLIMLVLSAPAGRWAQRHGARVPMTVGPL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHH
LGALGLVLLLRIGEEATYLTDVLPGVLVFGLGLAALVAPLTGAVLGAVPPTEAGVASGVN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHH
NAVARTAQLLAVAALPGLAGISGSLTDPAAFDAGFETAMWICVGLLVVGALLAAVLLRPV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAPRLDEVDCLPQCGVAGPALSPTRPSVVGESREK
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]