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The map label for this gene is echA21 [C]

Identifier: 54027301

GI number: 54027301

Start: 5629199

End: 5629981

Strand: Reverse

Name: echA21 [C]

Synonym: nfa53270

Alternate gene names: 54027301

Gene position: 5629981-5629199 (Counterclockwise)

Preceding gene: 54027302

Following gene: 54027300

Centisome position: 93.5

GC content: 72.03

Gene sequence:

>783_bases
ATGACCGACACCTCCACCGCGCCGCTGGTGCGCTACGACGTCCGCGACCGCTTCGCCGTGCTGACGTTCGATTCCCCGCA
CAACCGCAACGCGCTGTCCGCACGCCTGGTCGCGGACCTGCTGCGCGGTCTCGACGACGCGGCCGCGGACCCGGCGGTGC
GCGGCATCGTGCTCACCCACACCGGCAACACCTTCTGCGCGGGCGCCGACCTGAGCGAAGCGGGCGACACCGATCCCGCG
GTCGCCGCCGATCAGCGCACCCGCACGATGATCGGCGTGCTGCGCAGGCTCGTCGAGATCCCCAAGCCGGTGATCGCCCA
GATGGACGGCAACGTCCGCGCGGGCGGCATGGGCATCGTCGCGGGCTGCGACATCGTCGTCGCCGGGCCGGGCAGTTCGT
TCGCGCTCACCGAAGTGCGCATCGGGTTGGCGCCGTTCATGATCTCGCTCACCCTGCTGCCGCGCCTGGAACCGCGCGCC
GCCGCCCGCTACTACCTCACCGGGGAGAAGTTCGGCGCCGCCGAGGCCGAGCGGATCGGCTTGGTCACCGTTACCGCCGA
GGACCCCACCGCCGAGGTGGCGCGGCTGTGCGCGGAACTGCGCAAGGGCGCCCCGCAGGGCCTGGCCGAGGCCAAACGAC
TCGTCAACGCGCCCATCCTCGCCGAATTCGACCGGTCCGCCGAGGAACTGGCGCAACGCTCGGCGAGCTTCTTCGGCACC
CCCGAGGTGCGCGAGGGCATGACGGCCTTCCTGCAACGCCGCCCGCCCACCTGGGCAGAATGA

Upstream 100 bases:

>100_bases
TGAGCCGGGAGATGGTGCTGAACTTCGTGTCCCAGCACTCGCTCGGCCTGCCCAAGTCGTACTGATCCGCGCCGTACCGA
TCCGCCGGAAGGGGAGCACG

Downstream 100 bases:

>100_bases
CGATCATGGCGACACCCCACGAACCCAAGCAGGACCGCAGCCGCGCCACCCGGCAGCGGCTGCTCGAGGCGACCATCGAC
TGCCTGGCCGAGATGGGCTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTDTSTAPLVRYDVRDRFAVLTFDSPHNRNALSARLVADLLRGLDDAAADPAVRGIVLTHTGNTFCAGADLSEAGDTDPA
VAADQRTRTMIGVLRRLVEIPKPVIAQMDGNVRAGGMGIVAGCDIVVAGPGSSFALTEVRIGLAPFMISLTLLPRLEPRA
AARYYLTGEKFGAAEAERIGLVTVTAEDPTAEVARLCAELRKGAPQGLAEAKRLVNAPILAEFDRSAEELAQRSASFFGT
PEVREGMTAFLQRRPPTWAE

Sequences:

>Translated_260_residues
MTDTSTAPLVRYDVRDRFAVLTFDSPHNRNALSARLVADLLRGLDDAAADPAVRGIVLTHTGNTFCAGADLSEAGDTDPA
VAADQRTRTMIGVLRRLVEIPKPVIAQMDGNVRAGGMGIVAGCDIVVAGPGSSFALTEVRIGLAPFMISLTLLPRLEPRA
AARYYLTGEKFGAAEAERIGLVTVTAEDPTAEVARLCAELRKGAPQGLAEAKRLVNAPILAEFDRSAEELAQRSASFFGT
PEVREGMTAFLQRRPPTWAE
>Mature_259_residues
TDTSTAPLVRYDVRDRFAVLTFDSPHNRNALSARLVADLLRGLDDAAADPAVRGIVLTHTGNTFCAGADLSEAGDTDPAV
AADQRTRTMIGVLRRLVEIPKPVIAQMDGNVRAGGMGIVAGCDIVVAGPGSSFALTEVRIGLAPFMISLTLLPRLEPRAA
ARYYLTGEKFGAAEAERIGLVTVTAEDPTAEVARLCAELRKGAPQGLAEAKRLVNAPILAEFDRSAEELAQRSASFFGTP
EVREGMTAFLQRRPPTWAE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=30.327868852459, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=28.0155642023346, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=32.2916666666667, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI221136756, Length=242, Percent_Identity=25.6198347107438, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=242, Percent_Identity=25.6198347107438, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221307494, Length=242, Percent_Identity=25.6198347107438, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=247, Percent_Identity=31.1740890688259, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=29.0076335877863, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=31.0756972111554, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI221142681, Length=246, Percent_Identity=28.4552845528455, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788682, Length=172, Percent_Identity=28.4883720930233, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=30.8300395256917, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=27.5303643724696, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=26.0504201680672, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=25.7692307692308, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=22.093023255814, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=186, Percent_Identity=30.1075268817204, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=28.0373831775701, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=28.8065843621399, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=28.8065843621399, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922422, Length=264, Percent_Identity=25, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654903, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583165, Length=263, Percent_Identity=26.615969581749, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19920382, Length=171, Percent_Identity=29.8245614035088, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27716; Mature: 27585

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTSTAPLVRYDVRDRFAVLTFDSPHNRNALSARLVADLLRGLDDAAADPAVRGIVLTH
CCCCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCHHEEEEEEE
TGNTFCAGADLSEAGDTDPAVAADQRTRTMIGVLRRLVEIPKPVIAQMDGNVRAGGMGIV
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEECCEEEE
AGCDIVVAGPGSSFALTEVRIGLAPFMISLTLLPRLEPRAAARYYLTGEKFGAAEAERIG
ECCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCCCCCCEE
LVTVTAEDPTAEVARLCAELRKGAPQGLAEAKRLVNAPILAEFDRSAEELAQRSASFFGT
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCC
PEVREGMTAFLQRRPPTWAE
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTSTAPLVRYDVRDRFAVLTFDSPHNRNALSARLVADLLRGLDDAAADPAVRGIVLTH
CCCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHCCCCHHEEEEEEE
TGNTFCAGADLSEAGDTDPAVAADQRTRTMIGVLRRLVEIPKPVIAQMDGNVRAGGMGIV
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEECCEEEE
AGCDIVVAGPGSSFALTEVRIGLAPFMISLTLLPRLEPRAAARYYLTGEKFGAAEAERIG
ECCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCCCCCCEE
LVTVTAEDPTAEVARLCAELRKGAPQGLAEAKRLVNAPILAEFDRSAEELAQRSASFFGT
EEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCC
PEVREGMTAFLQRRPPTWAE
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA