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The map label for this gene is pucL [H]

Identifier: 54027216

GI number: 54027216

Start: 5542682

End: 5543575

Strand: Reverse

Name: pucL [H]

Synonym: nfa52420

Alternate gene names: 54027216

Gene position: 5543575-5542682 (Counterclockwise)

Preceding gene: 54027217

Following gene: 54027215

Centisome position: 92.07

GC content: 70.81

Gene sequence:

>894_bases
GTGCCGCTGACCGGACCGATCGTGCTGTCGGACAATCGCTACGGCAAGTCCGAGAACCGCATCGTGCGCATCCACCGGGA
CGGGCCGCGGCACGAGATCCGCGACGTCACCGTCTCCACCCGGCTGCGCGGTGATTTCGCCGACGCCTACGTCACCGGCG
ACCAGTCGGCGGTGCTGCCCACCGACAGCCAGAAGCAGACCGCCTACGCCTACGCCAAGACCCAGGGCCTGCACCACATC
GAGGACTACGGGCTCGCCTTGGCCGAGCATTTCGTCCGCGACATCGCCCCGGTCCACGCGGCCAGGGTGGAGATCGACGA
ATTCGCCTGGGAGCGCGCGCGGGTCGGGCCGGGCGAGCACGACCACACCTGGGTGCGGCGGGGCCCGGAGGTGCGCACGG
CGGCGGTGACGGTCACGGGCACCGGGTCCTGGGTGGTCGGCGGCGTGCGGGATCTGGTGCTGCTCAAATCGACCGGATCG
GAGTTCGCGGGCTTCCTGCGCGACGAATTCACCGTGCTCGCGCCGACCGACGACCGGATGCTGGCCACCGCCCTGACCGC
GCGGTGGCGCTTCGCCGACACGGCGGCCGTGGACTGGGCGGCCACGTATACGGGCGTGCGCGCCCGATTGATCGAGGTGT
TCGCCACCCACCATTCCAAGGCGTTGCAACAGACGCTGTTCGAGATGGGCTCGGCGGTGCTGGCCGACTTCCCGGTGCTG
GCCGAGATCCGGCTGGTCGCGCCCAACAGACACCACTTCGACTACGACCTGTCGCCGTTCGGGATCGCCAACCACGGCGA
GGTGTACTACGCCGCCGACCGGCCCTACGGGCTCATCGAGGCCGCGCTGCGCCGCGCCGACGCGCCGGACGCCGGGCAGG
CATGGGAGCTGTGA

Upstream 100 bases:

>100_bases
GGTCGCGATCACCTTCGCGGTGACCGAGGAACGTCACCACCACGTGCCGTTGCTGCTGTCGCCCTTCGCTTTTTCCACCT
ACCGAGGGAGTTGAGCGCCC

Downstream 100 bases:

>100_bases
ACGCGCCGACGCTGGTGCTGGACCGTTGCGCGGTCGTCACCGTCGACGCCGCGGGTACGGAGTACCGCGAGGGCCACGTC
GTGGTGCACGGCAACCGGAT

Product: putative uricase

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPLTGPIVLSDNRYGKSENRIVRIHRDGPRHEIRDVTVSTRLRGDFADAYVTGDQSAVLPTDSQKQTAYAYAKTQGLHHI
EDYGLALAEHFVRDIAPVHAARVEIDEFAWERARVGPGEHDHTWVRRGPEVRTAAVTVTGTGSWVVGGVRDLVLLKSTGS
EFAGFLRDEFTVLAPTDDRMLATALTARWRFADTAAVDWAATYTGVRARLIEVFATHHSKALQQTLFEMGSAVLADFPVL
AEIRLVAPNRHHFDYDLSPFGIANHGEVYYAADRPYGLIEAALRRADAPDAGQAWEL

Sequences:

>Translated_297_residues
MPLTGPIVLSDNRYGKSENRIVRIHRDGPRHEIRDVTVSTRLRGDFADAYVTGDQSAVLPTDSQKQTAYAYAKTQGLHHI
EDYGLALAEHFVRDIAPVHAARVEIDEFAWERARVGPGEHDHTWVRRGPEVRTAAVTVTGTGSWVVGGVRDLVLLKSTGS
EFAGFLRDEFTVLAPTDDRMLATALTARWRFADTAAVDWAATYTGVRARLIEVFATHHSKALQQTLFEMGSAVLADFPVL
AEIRLVAPNRHHFDYDLSPFGIANHGEVYYAADRPYGLIEAALRRADAPDAGQAWEL
>Mature_296_residues
PLTGPIVLSDNRYGKSENRIVRIHRDGPRHEIRDVTVSTRLRGDFADAYVTGDQSAVLPTDSQKQTAYAYAKTQGLHHIE
DYGLALAEHFVRDIAPVHAARVEIDEFAWERARVGPGEHDHTWVRRGPEVRTAAVTVTGTGSWVVGGVRDLVLLKSTGSE
FAGFLRDEFTVLAPTDDRMLATALTARWRFADTAAVDWAATYTGVRARLIEVFATHHSKALQQTLFEMGSAVLADFPVLA
EIRLVAPNRHHFDYDLSPFGIANHGEVYYAADRPYGLIEAALRRADAPDAGQAWEL

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3648

COG function: function code Q; Uricase (urate oxidase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Drosophila melanogaster, GI17136576, Length=314, Percent_Identity=28.6624203821656, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 32915; Mature: 32784

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00366 URICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTGPIVLSDNRYGKSENRIVRIHRDGPRHEIRDVTVSTRLRGDFADAYVTGDQSAVLP
CCCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHEEEEEEEECCCCCCEEEECCCCEECC
TDSQKQTAYAYAKTQGLHHIEDYGLALAEHFVRDIAPVHAARVEIDEFAWERARVGPGEH
CCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCCCCCC
DHTWVRRGPEVRTAAVTVTGTGSWVVGGVRDLVLLKSTGSEFAGFLRDEFTVLAPTDDRM
CCHHHHCCCCEEEEEEEEECCCCEEECCCEEEEEEECCCHHHHHHHHCCEEEECCCCCHH
LATALTARWRFADTAAVDWAATYTGVRARLIEVFATHHSKALQQTLFEMGSAVLADFPVL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AEIRLVAPNRHHFDYDLSPFGIANHGEVYYAADRPYGLIEAALRRADAPDAGQAWEL
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PLTGPIVLSDNRYGKSENRIVRIHRDGPRHEIRDVTVSTRLRGDFADAYVTGDQSAVLP
CCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHEEEEEEEECCCCCCEEEECCCCEECC
TDSQKQTAYAYAKTQGLHHIEDYGLALAEHFVRDIAPVHAARVEIDEFAWERARVGPGEH
CCCCCHHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCCCCCC
DHTWVRRGPEVRTAAVTVTGTGSWVVGGVRDLVLLKSTGSEFAGFLRDEFTVLAPTDDRM
CCHHHHCCCCEEEEEEEEECCCCEEECCCEEEEEEECCCHHHHHHHHCCEEEECCCCCHH
LATALTARWRFADTAAVDWAATYTGVRARLIEVFATHHSKALQQTLFEMGSAVLADFPVL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
AEIRLVAPNRHHFDYDLSPFGIANHGEVYYAADRPYGLIEAALRRADAPDAGQAWEL
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]