| Definition | Nocardia farcinica IFM 10152 chromosome, complete genome. |
|---|---|
| Accession | NC_006361 |
| Length | 6,021,225 |
Click here to switch to the map view.
The map label for this gene is metE [H]
Identifier: 54027202
GI number: 54027202
Start: 5521709
End: 5524012
Strand: Reverse
Name: metE [H]
Synonym: nfa52280
Alternate gene names: 54027202
Gene position: 5524012-5521709 (Counterclockwise)
Preceding gene: 54027203
Following gene: 54027199
Centisome position: 91.74
GC content: 69.62
Gene sequence:
>2304_bases ATGGTCAACGTCACCGAGGGCTACGGGTCGAGCATTCTGGGCTACCCCAGGATCGGGCCGCACCGGGAACTCAAGCGCGC GCTCGAGTCCTACTGGCGCGGGTCGCTCTCGCGGGAGGAACTGCTCGAGGTCGGCCGCGACATCCAGGAGCGCCAGTTCA ACGAGCTGGCCGCCACCGGCCTGACCCAGGTGCCCGGCAACACCTTCTCCTTCTACGACCACGTCCTGGACAACGCGCTG ATGTTCGGCGCGGTGCCCAAGCGCTTCCAGCCCTACCAGGACGTGATGGATCCGCTGGACTTCTACTTCCTGATGGCGCG CGGGCGGCCGGACCTGCCCCCGCTCGAGCTGGTGCGCTACATCAACACCAACTACCACTACCGCCAGCCCGAGCTCGCGC CCGACACCGAGTTCTCGCTGCACCCCGAGGCGCTGCTCGACGAATTCGACCGCGCGATGGCGCAGGGCATCGAACTGCGC CCGGTCGTGCTCGGACCCGCTTCGCTGCTGCTGCTGTCCAAGGCCGGTGACCAGCCGGGCGAGGCCGAGTTCGACACGCT GAGCCTGCTCGACAAGCTGTTGCCGGTGTACGAGGAACTGTTCGAGATCCTCGCCAAGCGCGGCGCCACCTGCGTGCAGC TCGACGAGCCCGCCTACACCAGCGAGCGCAGCGAGGCCGAGCTGGCCGCCTTCGAGCGCGCCTACCAGCGGCTGTCCAAG GCGCCGCTGCGTCCCCGCATCCTGGTCACCGGCCAGTACGGCGATTTCGGCGAGGCGCTGACCATCCTGGCGGGCACCGG CGTCGAGGCGATCGGCCTCGACCTGGCCAGCCACCGCATCGACCCCGACGAGCTGGCCAAGGTGCCCGGCATCCGCCGCA AGCGGCTGTACGCGGGCGTGATCAGCGGCCGCAACGTCTGGCGCGCCGACCGGTACGTGACGCTCGAATACCTCAACGAA CTCGCCAAGGTCTGCCCGGACCTGGTGGTCTCCACCGGCTGCACGCTGCTGCACGTGCCCTACGACCTGCTCGTCGAGTA CGACCTCGAGGGCAACGTCGCCGACCGGCTCGCCTTCGCCAAACAGAAGGTGGGCGAGGTGGTCTCGCTGGCCAAGGCGC TCACCGAGGGGCCGTCGGACAAGTGGCGCAAGCGTCCGCGCGAGGTGCACTTCAAGCAGAAGCACGCGGTGCGCCAGCGG GTGTACTCCATCACCCCGGACATGCGCTCGCGCGAGCCCTACGCCGAGCGGCGCGCCGCCCAGCAGGCCAGGCTGAACTT GCCGCCGGTGCCCGCCACCACGCTGGGCTCCTTCCCGCAGACCGGCAAGATCCGCCAGGCCCGCTACGACCTCGACCAGG GACGGCTGTCCTACGAGGAGTACCGCAAGCGGATCGAGGCCGAGATCGAGGCCACCATCCGGTTGCAGGAGGACATCGGC CTGGACGTGCTGGTGCACGGCGAGCACGAGCGCAACGACATGGTGCAGTACTTCGCCGAACTGCTCGACGGCTTCGCCAC CACCCACTTCGGCTGGGTGCAGGTGTACGGCTCGCGGTGTGTGCGGCCGCCGATCATCTACGGTGACGTCTCGCGCCCGG CGCCGATGTCGGTCGACTGGATCACCTACGCCCAGTCGCTGACCGACAAGCCGGTCAAGGGCATCGTCACCGGCCCGGTC ACCATGATCGCGCGCTCGTTCGTGCGCCAGGACCAGCCGCTCTACGAGACCGCCGACCAGGTGGCGCTGGCCATCCGTGA CGAGGTGGTGGATCTGGAGAAGGCCGGCATCGCGATCATCCAGGTCGACGAGCCCGCCATCCGCGAGATGCTGCCGCTGC GCGCCGAGGGCCGTGCCGAGTACCTGCGCTGGGCGGTCGGCGCGTTCCGGCTGGCCACCTCCGGCGTGCGCCCGGACACC CAGATCCACACGCACATCGGCTATTCCGGGCGCACCGAGGTGGTGGACGCGATCGAGGAACTCGACGCCGACGTCACCGC GATCGTGGCCACCCGCTCCATCGAGTGGGTGCTCAAGGCGCTCAAGGAGGACGCCGCGGGCGGCCACGGCCTCAGTCACG GCGTCGGCCCCGGCGTCTACGAGAGCCGCTCGGCCCGCATCCCCGACATCGACGAACTCGACGAGCTGCTGTCCGCCGCC GCCGAAGCCGTCACCCCGGAACGCCTGTGGGCCAACCCCGACGGTGGTCTCAAGACCCGCCACTACTGGCAGCTCGAGCC CTCCCTGCGCAACATGGTCGCCGCCGCCCGCCGCATCCGCAGGCGGGTGCAGGCCATGGACTGA
Upstream 100 bases:
>100_bases TCCCCGCGGCGGCCGACATAAGCTGCAAAGACGAGAGCGCAGCGCTGCGCGCGGCCGCCCGGCGGCGGGCACGCAGCCGA TCGAAAGGGAGTTCCCGCGC
Downstream 100 bases:
>100_bases TTCCGCAGGCGCTCCGGCGAGCGGGCCGCTTCTCCACGGAGAGGCGGCCCGCTCGCCGCGTCAGGGGGAGGTGCGGCCGG GGGAGTCGGTGCCGGTGAAG
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 767; Mature: 767
Protein sequence:
>767_residues MVNVTEGYGSSILGYPRIGPHRELKRALESYWRGSLSREELLEVGRDIQERQFNELAATGLTQVPGNTFSFYDHVLDNAL MFGAVPKRFQPYQDVMDPLDFYFLMARGRPDLPPLELVRYINTNYHYRQPELAPDTEFSLHPEALLDEFDRAMAQGIELR PVVLGPASLLLLSKAGDQPGEAEFDTLSLLDKLLPVYEELFEILAKRGATCVQLDEPAYTSERSEAELAAFERAYQRLSK APLRPRILVTGQYGDFGEALTILAGTGVEAIGLDLASHRIDPDELAKVPGIRRKRLYAGVISGRNVWRADRYVTLEYLNE LAKVCPDLVVSTGCTLLHVPYDLLVEYDLEGNVADRLAFAKQKVGEVVSLAKALTEGPSDKWRKRPREVHFKQKHAVRQR VYSITPDMRSREPYAERRAAQQARLNLPPVPATTLGSFPQTGKIRQARYDLDQGRLSYEEYRKRIEAEIEATIRLQEDIG LDVLVHGEHERNDMVQYFAELLDGFATTHFGWVQVYGSRCVRPPIIYGDVSRPAPMSVDWITYAQSLTDKPVKGIVTGPV TMIARSFVRQDQPLYETADQVALAIRDEVVDLEKAGIAIIQVDEPAIREMLPLRAEGRAEYLRWAVGAFRLATSGVRPDT QIHTHIGYSGRTEVVDAIEELDADVTAIVATRSIEWVLKALKEDAAGGHGLSHGVGPGVYESRSARIPDIDELDELLSAA AEAVTPERLWANPDGGLKTRHYWQLEPSLRNMVAAARRIRRRVQAMD
Sequences:
>Translated_767_residues MVNVTEGYGSSILGYPRIGPHRELKRALESYWRGSLSREELLEVGRDIQERQFNELAATGLTQVPGNTFSFYDHVLDNAL MFGAVPKRFQPYQDVMDPLDFYFLMARGRPDLPPLELVRYINTNYHYRQPELAPDTEFSLHPEALLDEFDRAMAQGIELR PVVLGPASLLLLSKAGDQPGEAEFDTLSLLDKLLPVYEELFEILAKRGATCVQLDEPAYTSERSEAELAAFERAYQRLSK APLRPRILVTGQYGDFGEALTILAGTGVEAIGLDLASHRIDPDELAKVPGIRRKRLYAGVISGRNVWRADRYVTLEYLNE LAKVCPDLVVSTGCTLLHVPYDLLVEYDLEGNVADRLAFAKQKVGEVVSLAKALTEGPSDKWRKRPREVHFKQKHAVRQR VYSITPDMRSREPYAERRAAQQARLNLPPVPATTLGSFPQTGKIRQARYDLDQGRLSYEEYRKRIEAEIEATIRLQEDIG LDVLVHGEHERNDMVQYFAELLDGFATTHFGWVQVYGSRCVRPPIIYGDVSRPAPMSVDWITYAQSLTDKPVKGIVTGPV TMIARSFVRQDQPLYETADQVALAIRDEVVDLEKAGIAIIQVDEPAIREMLPLRAEGRAEYLRWAVGAFRLATSGVRPDT QIHTHIGYSGRTEVVDAIEELDADVTAIVATRSIEWVLKALKEDAAGGHGLSHGVGPGVYESRSARIPDIDELDELLSAA AEAVTPERLWANPDGGLKTRHYWQLEPSLRNMVAAARRIRRRVQAMD >Mature_767_residues MVNVTEGYGSSILGYPRIGPHRELKRALESYWRGSLSREELLEVGRDIQERQFNELAATGLTQVPGNTFSFYDHVLDNAL MFGAVPKRFQPYQDVMDPLDFYFLMARGRPDLPPLELVRYINTNYHYRQPELAPDTEFSLHPEALLDEFDRAMAQGIELR PVVLGPASLLLLSKAGDQPGEAEFDTLSLLDKLLPVYEELFEILAKRGATCVQLDEPAYTSERSEAELAAFERAYQRLSK APLRPRILVTGQYGDFGEALTILAGTGVEAIGLDLASHRIDPDELAKVPGIRRKRLYAGVISGRNVWRADRYVTLEYLNE LAKVCPDLVVSTGCTLLHVPYDLLVEYDLEGNVADRLAFAKQKVGEVVSLAKALTEGPSDKWRKRPREVHFKQKHAVRQR VYSITPDMRSREPYAERRAAQQARLNLPPVPATTLGSFPQTGKIRQARYDLDQGRLSYEEYRKRIEAEIEATIRLQEDIG LDVLVHGEHERNDMVQYFAELLDGFATTHFGWVQVYGSRCVRPPIIYGDVSRPAPMSVDWITYAQSLTDKPVKGIVTGPV TMIARSFVRQDQPLYETADQVALAIRDEVVDLEKAGIAIIQVDEPAIREMLPLRAEGRAEYLRWAVGAFRLATSGVRPDT QIHTHIGYSGRTEVVDAIEELDADVTAIVATRSIEWVLKALKEDAAGGHGLSHGVGPGVYESRSARIPDIDELDELLSAA AEAVTPERLWANPDGGLKTRHYWQLEPSLRNMVAAARRIRRRVQAMD
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=767, Percent_Identity=44.7196870925684, Blast_Score=592, Evalue=1e-170, Organism=Saccharomyces cerevisiae, GI6320936, Length=778, Percent_Identity=42.2879177377892, Blast_Score=545, Evalue=1e-156,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 86151; Mature: 86151
Theoretical pI: Translated: 5.43; Mature: 5.43
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVNVTEGYGSSILGYPRIGPHRELKRALESYWRGSLSREELLEVGRDIQERQFNELAATG CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHCC LTQVPGNTFSFYDHVLDNALMFGAVPKRFQPYQDVMDPLDFYFLMARGRPDLPPLELVRY CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH INTNYHYRQPELAPDTEFSLHPEALLDEFDRAMAQGIELRPVVLGPASLLLLSKAGDQPG HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHEECCCCCCC EAEFDTLSLLDKLLPVYEELFEILAKRGATCVQLDEPAYTSERSEAELAAFERAYQRLSK CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC APLRPRILVTGQYGDFGEALTILAGTGVEAIGLDLASHRIDPDELAKVPGIRRKRLYAGV CCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHH ISGRNVWRADRYVTLEYLNELAKVCPDLVVSTGCTLLHVPYDLLVEYDLEGNVADRLAFA HCCCCEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEECCHHHEEEECCCCCHHHHHHHH KQKVGEVVSLAKALTEGPSDKWRKRPREVHFKQKHAVRQRVYSITPDMRSREPYAERRAA HHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH QQARLNLPPVPATTLGSFPQTGKIRQARYDLDQGRLSYEEYRKRIEAEIEATIRLQEDIG HHHCCCCCCCCCHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCCC LDVLVHGEHERNDMVQYFAELLDGFATTHFGWVQVYGSRCVRPPIIYGDVSRPAPMSVDW CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHH ITYAQSLTDKPVKGIVTGPVTMIARSFVRQDQPLYETADQVALAIRDEVVDLEKAGIAII HHHHHHHCCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE QVDEPAIREMLPLRAEGRAEYLRWAVGAFRLATSGVRPDTQIHTHIGYSGRTEVVDAIEE EECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH LDADVTAIVATRSIEWVLKALKEDAAGGHGLSHGVGPGVYESRSARIPDIDELDELLSAA HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH AEAVTPERLWANPDGGLKTRHYWQLEPSLRNMVAAARRIRRRVQAMD HHHCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MVNVTEGYGSSILGYPRIGPHRELKRALESYWRGSLSREELLEVGRDIQERQFNELAATG CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHCC LTQVPGNTFSFYDHVLDNALMFGAVPKRFQPYQDVMDPLDFYFLMARGRPDLPPLELVRY CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH INTNYHYRQPELAPDTEFSLHPEALLDEFDRAMAQGIELRPVVLGPASLLLLSKAGDQPG HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHEECCCCCCC EAEFDTLSLLDKLLPVYEELFEILAKRGATCVQLDEPAYTSERSEAELAAFERAYQRLSK CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHC APLRPRILVTGQYGDFGEALTILAGTGVEAIGLDLASHRIDPDELAKVPGIRRKRLYAGV CCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHH ISGRNVWRADRYVTLEYLNELAKVCPDLVVSTGCTLLHVPYDLLVEYDLEGNVADRLAFA HCCCCEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEECCHHHEEEECCCCCHHHHHHHH KQKVGEVVSLAKALTEGPSDKWRKRPREVHFKQKHAVRQRVYSITPDMRSREPYAERRAA HHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH QQARLNLPPVPATTLGSFPQTGKIRQARYDLDQGRLSYEEYRKRIEAEIEATIRLQEDIG HHHCCCCCCCCCHHCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEECCCCC LDVLVHGEHERNDMVQYFAELLDGFATTHFGWVQVYGSRCVRPPIIYGDVSRPAPMSVDW CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHH ITYAQSLTDKPVKGIVTGPVTMIARSFVRQDQPLYETADQVALAIRDEVVDLEKAGIAII HHHHHHHCCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE QVDEPAIREMLPLRAEGRAEYLRWAVGAFRLATSGVRPDTQIHTHIGYSGRTEVVDAIEE EECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHH LDADVTAIVATRSIEWVLKALKEDAAGGHGLSHGVGPGVYESRSARIPDIDELDELLSAA HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH AEAVTPERLWANPDGGLKTRHYWQLEPSLRNMVAAARRIRRRVQAMD HHHCCHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA