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The map label for this gene is galE [C]

Identifier: 54027149

GI number: 54027149

Start: 5473542

End: 5474615

Strand: Reverse

Name: galE [C]

Synonym: nfa51750

Alternate gene names: 54027149

Gene position: 5474615-5473542 (Counterclockwise)

Preceding gene: 54027150

Following gene: 54027148

Centisome position: 90.92

GC content: 67.88

Gene sequence:

>1074_bases
GTGGGTTCTGAGGCGAGGGACGCACACGCACCCAAGGTGGTGCTGGTCACCGGTGCGAGCCGATTCTTCGGCGGGCACGT
CGTCGCCGAACTCGCCCAGGACCCGGCGGTGGAACGTATCATCGCGGTGGACACCATGACGCCGGGCCGCGAGCTGCAAC
GCCGCATGGGGCGCGCCGAATTCGTGCGCGCCGATATTCGGAATCCGTTGATCCGCAAGGTGATCGGAGGCAACGAGGTG
GACACTGTGGTGCATACCGCGGTGCTGTCCCGTCCGCCGTCGCCCGGTGGCCGCGCGGTGATGAAAGATCTCAACGTCCT
CGGCGCCATGCAACTGTTCGCCGTCTGCCAGAAAACCCCGTCGGTTCGTAGAGTGGTCGTTCGCTCGAGTTCGGCGGTGT
ACGGCTGCAGTCCGAAGGATCCGGCGAAATTCACCGAGGAAATGAGTGCGCGTAAGCCGCCGAGCGGGTGGTTCGCGCGC
GACATGATCGAAATCGAAGGATTCGTACGCGGCATGGCCCGGCGCCGTCCCGATATCGGCGCCGCCATTCTCCGCCTGGC
GCCCACGGTGGGGCCGCGGCTGGCCCGGCGCGGCGTGCAGTACCTGCGCTGGCCGGTCGCGCCGACGGTCTTCGGCCGCG
ATGCCAGGATGCAGCTGCTGCACGAGCAGGACGCGGTCGCGGCTCTGGCGCACGCCGCCCGCACCGCCCGCGGTGGCACC
TACAACGTCGCGGGCGACGGCGCGCTCACGCTCTCGCAAGCCGTGCGTCGTTCGGGCAGACTGGAACTACCGCTTCCGTT
CGCCGTGTTCCGCACCGCGGGGCGCGCGCTGATGGAAACGGTGATGCGTGACTTCAGTGCCGAACAGCTCGACTACTTCC
ATTTCGGGTGTGGCCTGGACACCACCCGCATGCGGACCGAACTCGGGTTCGAGCCGCGGTGGACGACCGTTCAGGCGTTC
GACGACTTCATCGGGGGCGCAGCGCTGCGACCCGTCATCGATCCGCGCTGGATCGATGCCGCAGAACACAGACTGCTCGG
CCTGCTCGGGGCCGGTACGGGAGCACACACATGA

Upstream 100 bases:

>100_bases
TCATCGTTAGATCCTCGTAAATACTCTCCGCGATCGCGGAGTTCAGCGGTTACTCTTGTGACGAATAATCAACCGGGGGC
CTGGCAGGTGGGATTCGCAC

Downstream 100 bases:

>100_bases
ACGACGTTGCCAAAGTTATCCACCTGCACGACGTGACCACCGAGACCCGGCCGCGTCCGGCCGCCCGGCGCTGGCCGGAG
CCGCCGCCCGGCGCACCCGC

Product: hypothetical protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MGSEARDAHAPKVVLVTGASRFFGGHVVAELAQDPAVERIIAVDTMTPGRELQRRMGRAEFVRADIRNPLIRKVIGGNEV
DTVVHTAVLSRPPSPGGRAVMKDLNVLGAMQLFAVCQKTPSVRRVVVRSSSAVYGCSPKDPAKFTEEMSARKPPSGWFAR
DMIEIEGFVRGMARRRPDIGAAILRLAPTVGPRLARRGVQYLRWPVAPTVFGRDARMQLLHEQDAVAALAHAARTARGGT
YNVAGDGALTLSQAVRRSGRLELPLPFAVFRTAGRALMETVMRDFSAEQLDYFHFGCGLDTTRMRTELGFEPRWTTVQAF
DDFIGGAALRPVIDPRWIDAAEHRLLGLLGAGTGAHT

Sequences:

>Translated_357_residues
MGSEARDAHAPKVVLVTGASRFFGGHVVAELAQDPAVERIIAVDTMTPGRELQRRMGRAEFVRADIRNPLIRKVIGGNEV
DTVVHTAVLSRPPSPGGRAVMKDLNVLGAMQLFAVCQKTPSVRRVVVRSSSAVYGCSPKDPAKFTEEMSARKPPSGWFAR
DMIEIEGFVRGMARRRPDIGAAILRLAPTVGPRLARRGVQYLRWPVAPTVFGRDARMQLLHEQDAVAALAHAARTARGGT
YNVAGDGALTLSQAVRRSGRLELPLPFAVFRTAGRALMETVMRDFSAEQLDYFHFGCGLDTTRMRTELGFEPRWTTVQAF
DDFIGGAALRPVIDPRWIDAAEHRLLGLLGAGTGAHT
>Mature_356_residues
GSEARDAHAPKVVLVTGASRFFGGHVVAELAQDPAVERIIAVDTMTPGRELQRRMGRAEFVRADIRNPLIRKVIGGNEVD
TVVHTAVLSRPPSPGGRAVMKDLNVLGAMQLFAVCQKTPSVRRVVVRSSSAVYGCSPKDPAKFTEEMSARKPPSGWFARD
MIEIEGFVRGMARRRPDIGAAILRLAPTVGPRLARRGVQYLRWPVAPTVFGRDARMQLLHEQDAVAALAHAARTARGGTY
NVAGDGALTLSQAVRRSGRLELPLPFAVFRTAGRALMETVMRDFSAEQLDYFHFGCGLDTTRMRTELGFEPRWTTVQAFD
DFIGGAALRPVIDPRWIDAAEHRLLGLLGAGTGAHT

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=190, Percent_Identity=28.9473684210526, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38927; Mature: 38796

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEARDAHAPKVVLVTGASRFFGGHVVAELAQDPAVERIIAVDTMTPGRELQRRMGRAE
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHEEEECCCCCCHHHHHHCCHHH
FVRADIRNPLIRKVIGGNEVDTVVHTAVLSRPPSPGGRAVMKDLNVLGAMQLFAVCQKTP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
SVRRVVVRSSSAVYGCSPKDPAKFTEEMSARKPPSGWFARDMIEIEGFVRGMARRRPDIG
HHHHHHEECCCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
AAILRLAPTVGPRLARRGVQYLRWPVAPTVFGRDARMQLLHEQDAVAALAHAARTARGGT
HHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
YNVAGDGALTLSQAVRRSGRLELPLPFAVFRTAGRALMETVMRDFSAEQLDYFHFGCGLD
EEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCC
TTRMRTELGFEPRWTTVQAFDDFIGGAALRPVIDPRWIDAAEHRLLGLLGAGTGAHT
HHHHHHHCCCCCCCCHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GSEARDAHAPKVVLVTGASRFFGGHVVAELAQDPAVERIIAVDTMTPGRELQRRMGRAE
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHEEEECCCCCCHHHHHHCCHHH
FVRADIRNPLIRKVIGGNEVDTVVHTAVLSRPPSPGGRAVMKDLNVLGAMQLFAVCQKTP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
SVRRVVVRSSSAVYGCSPKDPAKFTEEMSARKPPSGWFARDMIEIEGFVRGMARRRPDIG
HHHHHHEECCCEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
AAILRLAPTVGPRLARRGVQYLRWPVAPTVFGRDARMQLLHEQDAVAALAHAARTARGGT
HHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
YNVAGDGALTLSQAVRRSGRLELPLPFAVFRTAGRALMETVMRDFSAEQLDYFHFGCGLD
EEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCC
TTRMRTELGFEPRWTTVQAFDDFIGGAALRPVIDPRWIDAAEHRLLGLLGAGTGAHT
HHHHHHHCCCCCCCCHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]