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The map label for this gene is menD

Identifier: 54027107

GI number: 54027107

Start: 5435269

End: 5436906

Strand: Reverse

Name: menD

Synonym: nfa51330

Alternate gene names: 54027107

Gene position: 5436906-5435269 (Counterclockwise)

Preceding gene: 54027108

Following gene: 54027105

Centisome position: 90.3

GC content: 74.18

Gene sequence:

>1638_bases
GTGAACCCGTCAACTGCGCAGGCCCGAGTTGTCGTCGACGAACTGGTTCGCGGCGGCGTCCGCGACGTGGTGCTGTGCCC
GGGGTCGCGCAACGCGCCGCTGGCGTTCGCGTTGCAGGCCGCCGACGCGGCGGGGCGGCTGCGGCTGCACATGCGCATCG
ATGAGCGCACGGCCGGATTCCTGGCGATCGGCCTGGCGATCTCGGCGGGTGCGCCGGTGCCGGTGGTGATGACCTCGGGC
ACCGCCGTCGCGAATCTCGGACCGGCGGTGCTGGAGGCCAATTACGCGCGGGTGCCGCTGATCGTGTTGAGCGCCAACCG
GCCCTACGAGATGCTCGGCACCGGCGCCAACCAGACGGTGGAACAGCTCGGCCTGTTCGGCAGCCAGGTGCGGGCCACCA
TCTCCCTCGGCCTCGCCGAAGCCGACGGTGCGGGTGCGGGCTCCTACGACCAGCAGAACAGCGTGTGGCGTTCGGCGGTG
TGCCGGGTGCTGGCCGCGGCCCGCGGCACCCGCTCGGGCAACGCCGGGCCGGTGCACTTCGACATCCCGCTGCGCGAGCC
GCTGGTGCCCGACCTCGCCGAGGGCGAAGCCGAGCCTGCGGGCCGGGCGGGCGGCGCGCCGTGGACGGCCACCCAGTACG
CCACCCTCGACGTGCCGCTCGAGATCGACCTGACCCCGGACACGGTCGTCGTGTCCGGGCACGGCGCGGGTCTGCGTCCC
GAACTCGCGCATCTGCCCACGGTCGCCGAACCCACCGCGCCCATGCACGGCCCGGCCCTGCATCCGCTGGCGCTGCCGCT
GCTCACGCCGCGCCAGGCGATCATCACCGGCCGCCCCACCCTGCACCGGCAGGTGTCGCGCCTGCTGGCCGACCCGGCGG
TCACGGTCTACGCCCTCACCACCGGCCCGCGCTGGCCCGACGTGTCCGGCAACGTCGTGGGCACCGGCACCAGGGCGGTC
ACCTCGGGCGGCCCGCGGCCGGAGTGGTTGGCGCACTGCCGTGAACTCGACGAGAAGGCCCGCCAGGTGGTCCGCGCCGA
ACTGGCCGGGCATCCCAAGCCGACCGGCCTGCATGTGGCCGCGGTGGTGATGGATGCGCTGCGCGAAGGCGATCAGCTGC
TGCTCGGCGCTTCCAACCCGGTGCGCGACGCGGCACTGGTGGCCACCCCGCGGCCGGGGGTGCGGGTGCTGTCCAACCGC
GGCGTGGCCGGCATCGACGGCACGGTCTCCACCGCGGTCGGCGCCGCCCTCGCCCACCCCGGCCGCACCATCGCCCTGAT
CGGCGACCTGACCTTCCTGCACGACGCGGCCGGGCTGCTGATCGGGCGCGGCGAACCGCGCCCCGCCGATCTGACCATCG
TCGTCGCCAACGACGACGGTGGCGGCATCTTCGAACTGCTCGAACAGGGCGACCCGCAGTACGCGGGCGTGTTCGAGCGC
GTGTTCGGCACCCCGCACGGGATGGACCTGGCGGCGCTGTGCGCGGCCTATCGCATCCCGCACCGCCAGGTGGACCCGGC
CGAACTCGCCGCGGAACTGGCCGGTGACGCGCACGGCCTGCGCGTGCTCGAGGTGATCACCGAGCGCTCCAGCCTCCGGG
AGCTGCACGCCACCGTGCGCGCCAAGATCGCTCCCTGA

Upstream 100 bases:

>100_bases
GCCGGAGACCGCGGCGGCCGGCCCTCGGCGGGCCGACTGCCGCGGTCTGTGTGACCAGGGTGACCGCGCTCGGGGCGGGT
CGATCTGACAGGCTGGAGGC

Downstream 100 bases:

>100_bases
GGCTGAAACGGCTCCCCGAAGCTGCGGACGGGGACTACTCGACCTCGCGGTCCTCGTCGTCCATCGGCACCGGGATCGAC
TGTTCGAGGACGTCGGCGGG

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNPSTAQARVVVDELVRGGVRDVVLCPGSRNAPLAFALQAADAAGRLRLHMRIDERTAGFLAIGLAISAGAPVPVVMTSG
TAVANLGPAVLEANYARVPLIVLSANRPYEMLGTGANQTVEQLGLFGSQVRATISLGLAEADGAGAGSYDQQNSVWRSAV
CRVLAAARGTRSGNAGPVHFDIPLREPLVPDLAEGEAEPAGRAGGAPWTATQYATLDVPLEIDLTPDTVVVSGHGAGLRP
ELAHLPTVAEPTAPMHGPALHPLALPLLTPRQAIITGRPTLHRQVSRLLADPAVTVYALTTGPRWPDVSGNVVGTGTRAV
TSGGPRPEWLAHCRELDEKARQVVRAELAGHPKPTGLHVAAVVMDALREGDQLLLGASNPVRDAALVATPRPGVRVLSNR
GVAGIDGTVSTAVGAALAHPGRTIALIGDLTFLHDAAGLLIGRGEPRPADLTIVVANDDGGGIFELLEQGDPQYAGVFER
VFGTPHGMDLAALCAAYRIPHRQVDPAELAAELAGDAHGLRVLEVITERSSLRELHATVRAKIAP

Sequences:

>Translated_545_residues
MNPSTAQARVVVDELVRGGVRDVVLCPGSRNAPLAFALQAADAAGRLRLHMRIDERTAGFLAIGLAISAGAPVPVVMTSG
TAVANLGPAVLEANYARVPLIVLSANRPYEMLGTGANQTVEQLGLFGSQVRATISLGLAEADGAGAGSYDQQNSVWRSAV
CRVLAAARGTRSGNAGPVHFDIPLREPLVPDLAEGEAEPAGRAGGAPWTATQYATLDVPLEIDLTPDTVVVSGHGAGLRP
ELAHLPTVAEPTAPMHGPALHPLALPLLTPRQAIITGRPTLHRQVSRLLADPAVTVYALTTGPRWPDVSGNVVGTGTRAV
TSGGPRPEWLAHCRELDEKARQVVRAELAGHPKPTGLHVAAVVMDALREGDQLLLGASNPVRDAALVATPRPGVRVLSNR
GVAGIDGTVSTAVGAALAHPGRTIALIGDLTFLHDAAGLLIGRGEPRPADLTIVVANDDGGGIFELLEQGDPQYAGVFER
VFGTPHGMDLAALCAAYRIPHRQVDPAELAAELAGDAHGLRVLEVITERSSLRELHATVRAKIAP
>Mature_545_residues
MNPSTAQARVVVDELVRGGVRDVVLCPGSRNAPLAFALQAADAAGRLRLHMRIDERTAGFLAIGLAISAGAPVPVVMTSG
TAVANLGPAVLEANYARVPLIVLSANRPYEMLGTGANQTVEQLGLFGSQVRATISLGLAEADGAGAGSYDQQNSVWRSAV
CRVLAAARGTRSGNAGPVHFDIPLREPLVPDLAEGEAEPAGRAGGAPWTATQYATLDVPLEIDLTPDTVVVSGHGAGLRP
ELAHLPTVAEPTAPMHGPALHPLALPLLTPRQAIITGRPTLHRQVSRLLADPAVTVYALTTGPRWPDVSGNVVGTGTRAV
TSGGPRPEWLAHCRELDEKARQVVRAELAGHPKPTGLHVAAVVMDALREGDQLLLGASNPVRDAALVATPRPGVRVLSNR
GVAGIDGTVSTAVGAALAHPGRTIALIGDLTFLHDAAGLLIGRGEPRPADLTIVVANDDGGGIFELLEQGDPQYAGVFER
VFGTPHGMDLAALCAAYRIPHRQVDPAELAAELAGDAHGLRVLEVITERSSLRELHATVRAKIAP

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=185, Percent_Identity=38.9189189189189, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_NOCFA (Q5YPA6)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_121349.1
- ProteinModelPortal:   Q5YPA6
- GeneID:   3111276
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa51330
- NMPDR:   fig|247156.1.peg.4932
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- ProtClustDB:   PRK07449
- BioCyc:   NFAR247156:NFA51330-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 56577; Mature: 56577

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTAQARVVVDELVRGGVRDVVLCPGSRNAPLAFALQAADAAGRLRLHMRIDERTAGF
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCE
LAIGLAISAGAPVPVVMTSGTAVANLGPAVLEANYARVPLIVLSANRPYEMLGTGANQTV
EEEEEEEECCCCCEEEEECCCCHHHCCHHHHCCCCCEEEEEEEECCCCHHHHCCCCHHHH
EQLGLFGSQVRATISLGLAEADGAGAGSYDQQNSVWRSAVCRVLAAARGTRSGNAGPVHF
HHHHCCHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
DIPLREPLVPDLAEGEAEPAGRAGGAPWTATQYATLDVPLEIDLTPDTVVVSGHGAGLRP
ECCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEECEEEEECCCCEEEEECCCCCCCC
ELAHLPTVAEPTAPMHGPALHPLALPLLTPRQAIITGRPTLHRQVSRLLADPAVTVYALT
HHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEEE
TGPRWPDVSGNVVGTGTRAVTSGGPRPEWLAHCRELDEKARQVVRAELAGHPKPTGLHVA
CCCCCCCCCCCEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AVVMDALREGDQLLLGASNPVRDAALVATPRPGVRVLSNRGVAGIDGTVSTAVGAALAHP
HHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCC
GRTIALIGDLTFLHDAAGLLIGRGEPRPADLTIVVANDDGGGIFELLEQGDPQYAGVFER
CCEEEEEECHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHH
VFGTPHGMDLAALCAAYRIPHRQVDPAELAAELAGDAHGLRVLEVITERSSLRELHATVR
HHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKIAP
EECCC
>Mature Secondary Structure
MNPSTAQARVVVDELVRGGVRDVVLCPGSRNAPLAFALQAADAAGRLRLHMRIDERTAGF
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCE
LAIGLAISAGAPVPVVMTSGTAVANLGPAVLEANYARVPLIVLSANRPYEMLGTGANQTV
EEEEEEEECCCCCEEEEECCCCHHHCCHHHHCCCCCEEEEEEEECCCCHHHHCCCCHHHH
EQLGLFGSQVRATISLGLAEADGAGAGSYDQQNSVWRSAVCRVLAAARGTRSGNAGPVHF
HHHHCCHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
DIPLREPLVPDLAEGEAEPAGRAGGAPWTATQYATLDVPLEIDLTPDTVVVSGHGAGLRP
ECCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEECEEEEECCCCEEEEECCCCCCCC
ELAHLPTVAEPTAPMHGPALHPLALPLLTPRQAIITGRPTLHRQVSRLLADPAVTVYALT
HHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEEE
TGPRWPDVSGNVVGTGTRAVTSGGPRPEWLAHCRELDEKARQVVRAELAGHPKPTGLHVA
CCCCCCCCCCCEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
AVVMDALREGDQLLLGASNPVRDAALVATPRPGVRVLSNRGVAGIDGTVSTAVGAALAHP
HHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHCCC
GRTIALIGDLTFLHDAAGLLIGRGEPRPADLTIVVANDDGGGIFELLEQGDPQYAGVFER
CCEEEEEECHHHHHHHCCEEEECCCCCCCCEEEEEEECCCCHHHHHHHCCCCHHHHHHHH
VFGTPHGMDLAALCAAYRIPHRQVDPAELAAELAGDAHGLRVLEVITERSSLRELHATVR
HHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKIAP
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA