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The map label for this gene is purN [H]

Identifier: 54026961

GI number: 54026961

Start: 5201399

End: 5202046

Strand: Reverse

Name: purN [H]

Synonym: nfa49870

Alternate gene names: 54026961

Gene position: 5202046-5201399 (Counterclockwise)

Preceding gene: 54026962

Following gene: 54026960

Centisome position: 86.4

GC content: 71.14

Gene sequence:

>648_bases
GTGCCGACGGTATCGCCCCCGTCGAACGCACCGGCCACCGTCGTCGTGCTCGCCTCGGGCACCGGGTCGCTGCTGCGCGC
CCTGCTCGACGCGGCGAGCGCGCCCGGCTACCCGGCCGAGATCGTCGCCGTCGGCGTGGACCGGGTGTGCGCGGCCACCG
AGCACGCCGAGGCCGCGGGCGTCCCGCATTTCCGGGTGGCGCTGAAGGACTTCCCCGATCGCGGCGCCTGGGACACCGCG
CTCACCGAAGCCGTCGCCGCGTATCGCCCCGACCTGGTGGTGTCGGCCGGTTTCATGAAGATCCTCGGCCCCGCGTTCAT
GGACCGTTTCGGCGGACGAATCATCAACACCCATCCCGCGCTGCTGCCGTCCTTTCCCGGCGCGCACGGCGTGCGCGACG
CGCTCGCCTACGGGGTGCGGGTCACCGGCTCGACGGTGCATCTGGTCGATTCGGGTGTCGACACCGGCCCGATCCTGGCG
CAGGAACCCGTGCCGGTACTGCCCGACGACGACGAGGCCACCCTGCACGAGCGCATCAAGGTTGTCGAGCGACGGCTGTT
GACCGAGGTCGTGGCCGCCGTCGCCACGCGAGGCATTGTCTCCGACGGACGAAAGGCAGTAATTCCAGATGAGCGGGTGC
TCCGGTGA

Upstream 100 bases:

>100_bases
GGGCGAAGCGGGCACCGCTAGGCTGATCACCGGCGGTGATGTCCCCGCCGTCCGATCGACACGCAGGAGTAGAGCGCTGA
CGTCCTCGCACGCCCAGTGG

Downstream 100 bases:

>100_bases
GTGAACGTAAGCCGATTCGCAGGGCGCTGGTGAGCGTCTACGACAAGACCGGGCTCGTCGAGCTCGCGACCGGTCTGCAC
GCCGCGGGTGTGGAACTGGT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MPTVSPPSNAPATVVVLASGTGSLLRALLDAASAPGYPAEIVAVGVDRVCAATEHAEAAGVPHFRVALKDFPDRGAWDTA
LTEAVAAYRPDLVVSAGFMKILGPAFMDRFGGRIINTHPALLPSFPGAHGVRDALAYGVRVTGSTVHLVDSGVDTGPILA
QEPVPVLPDDDEATLHERIKVVERRLLTEVVAAVATRGIVSDGRKAVIPDERVLR

Sequences:

>Translated_215_residues
MPTVSPPSNAPATVVVLASGTGSLLRALLDAASAPGYPAEIVAVGVDRVCAATEHAEAAGVPHFRVALKDFPDRGAWDTA
LTEAVAAYRPDLVVSAGFMKILGPAFMDRFGGRIINTHPALLPSFPGAHGVRDALAYGVRVTGSTVHLVDSGVDTGPILA
QEPVPVLPDDDEATLHERIKVVERRLLTEVVAAVATRGIVSDGRKAVIPDERVLR
>Mature_214_residues
PTVSPPSNAPATVVVLASGTGSLLRALLDAASAPGYPAEIVAVGVDRVCAATEHAEAAGVPHFRVALKDFPDRGAWDTAL
TEAVAAYRPDLVVSAGFMKILGPAFMDRFGGRIINTHPALLPSFPGAHGVRDALAYGVRVTGSTVHLVDSGVDTGPILAQ
EPVPVLPDDDEATLHERIKVVERRLLTEVVAAVATRGIVSDGRKAVIPDERVLR

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=198, Percent_Identity=39.8989898989899, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI209869995, Length=198, Percent_Identity=39.8989898989899, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI209869993, Length=198, Percent_Identity=39.8989898989899, Blast_Score=135, Evalue=2e-32,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=37.3056994818653, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787483, Length=200, Percent_Identity=30.5, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=36.7567567567568, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24582400, Length=173, Percent_Identity=45.6647398843931, Blast_Score=146, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22447; Mature: 22316

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVSPPSNAPATVVVLASGTGSLLRALLDAASAPGYPAEIVAVGVDRVCAATEHAEAAG
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHEEEECHHHHHHHHHHHHHCC
VPHFRVALKDFPDRGAWDTALTEAVAAYRPDLVVSAGFMKILGPAFMDRFGGRIINTHPA
CCCEEHHHHCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCEEECCCCC
LLPSFPGAHGVRDALAYGVRVTGSTVHLVDSGVDTGPILAQEPVPVLPDDDEATLHERIK
CCCCCCCCCHHHHHHHHCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VVERRLLTEVVAAVATRGIVSDGRKAVIPDERVLR
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCC
>Mature Secondary Structure 
PTVSPPSNAPATVVVLASGTGSLLRALLDAASAPGYPAEIVAVGVDRVCAATEHAEAAG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHEEEECHHHHHHHHHHHHHCC
VPHFRVALKDFPDRGAWDTALTEAVAAYRPDLVVSAGFMKILGPAFMDRFGGRIINTHPA
CCCEEHHHHCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCEEECCCCC
LLPSFPGAHGVRDALAYGVRVTGSTVHLVDSGVDTGPILAQEPVPVLPDDDEATLHERIK
CCCCCCCCCHHHHHHHHCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VVERRLLTEVVAAVATRGIVSDGRKAVIPDERVLR
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]