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The map label for this gene is purH [H]

Identifier: 54026960

GI number: 54026960

Start: 5199831

End: 5201402

Strand: Reverse

Name: purH [H]

Synonym: nfa49860

Alternate gene names: 54026960

Gene position: 5201402-5199831 (Counterclockwise)

Preceding gene: 54026961

Following gene: 54026958

Centisome position: 86.38

GC content: 71.56

Gene sequence:

>1572_bases
GTGAGTGAACGTAAGCCGATTCGCAGGGCGCTGGTGAGCGTCTACGACAAGACCGGGCTCGTCGAGCTCGCGACCGGTCT
GCACGCCGCGGGTGTGGAACTGGTCTCGACCGGCTCCACCGCGGGCCGGATCGCCGACGCGGGCATTCCGGTGACCAAGG
TCGAGGAGCTGACCGGCTTCCCGGAGACCCTCGACGGCCGGGTCAAGACACTGCACCCGCGGGTGCACGCGGGCATCCTC
GCCGACACCCGCAAGCCCGAGCATCTCGACCAGCTCACCGAGCTCGGCGTCGAGGCGTTCCAACTGGTGGTGGTGAACCT
CTACCCGTTCACCCAGACGGTGGCCAGTGGCGCGAGCCCGGACGAGTGCGTGGAGCAGATCGATATCGGTGGCCCGTCGA
TGGTGCGCGCCGCGGCGAAGAACCACCCGTCGGTCGCCGTCGTCGTGGACGCGGGCGACTACGACGACGTGCTGGCCGCG
GTGCGCGCCGGCGGGTTCACGCTGGCGCAGCGGACCGCGTTGGCGGCCAAGGCTTTCCAGCACACCGCCGGCTACGACGT
GGCGGTGGCGAGCTGGCTGACCGGTGTGCTCGCCCCGAGTGTGGCCGACCAGCCGCCGAGCACCCCGTACCCGGCCTGGA
TCGGCGGCAGCTGGGAGCGCGCGGCGGTACTGCGCTACGGCGAGAACCCGCACCAGTCGGCGGCGCTGTACACCAGCAAC
GACGGCAGCCCGGGGCTGGCGCAGGCGCAGCAGTTGCACGGCAAGGAGATGTCGTACAACAACTACACCGATGCCGACGC
CGCCTGGCGCGCCGCCTACGACCACGAGGCCCCGGCCGTCGCGATCATCAAGCACGCCAACCCCTGCGGTATCGCGATCG
GCGCGGACATCGCCGAGGCGCACCGCAAGGCGCACGCCTGCGATCCGGTGAGCGCGTTCGGCGGCGTCATCGCCGCCAAT
CGCGAGGTCACCGTGGAGATGGCCGAGCAGGTCGCCGACATCTTCACCGAGGTGATCGTCGCCCCGTCCTACGCCGACGG
CGCGGTGGAGGTGCTGCAGCGCAAGAAGAACGTGCGCATCCTGATCGCCGAGCCGCCGCAGCGCGCCGGTGTGGAGCTGC
GCCCGATCAGCGGCGGCGCGCTGCTGCAACAGCGCGACATCCTCGACGCCGAGGGCGACGATCCGGCCAACTGGACGCTG
GTGGCCGGTACGGCCGCCGACGAGCAGACCCTCGCCGATCTGGCGTTCGCGTGGCGGGCGTGCCGCGCGGTGAAGTCCAA
CGCGATCCTGCTGGCCAGCGACGGCGCGTCGGTGGGCGTGGGCATGGGCCAGGTGAACCGGGTGGACGCGGTGCAGCTGG
CGGTGCGCCGCGCCGGTGACCGGGCCAAGGGCTCGGTGGCCGCCTCGGATGCCTTCTTCCCGTTCCCGGACGGTCCCCAG
CAGCTGATCCAGGCAGGTGTGCGCGCCATCGTGCAGCCCGGCGGTTCGGTGCGGGACCAGGAGACGATCGATCTCGCCCG
CGAGGCGGGCGTGACGCTCTACCTGACGGGGTCGCGGCACTTCGCGCACTGA

Upstream 100 bases:

>100_bases
TCGAGCGACGGCTGTTGACCGAGGTCGTGGCCGCCGTCGCCACGCGAGGCATTGTCTCCGACGGACGAAAGGCAGTAATT
CCAGATGAGCGGGTGCTCCG

Downstream 100 bases:

>100_bases
GTCCCTCGCGACGAGAGGCGGGTCCGGCGTACGGGCCCGCCTCTCGTCGTCTCCCGGGATGGTGCCGCCGATCAGACCGC
GCTGAGCACCTGCCGTGCGC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSERKPIRRALVSVYDKTGLVELATGLHAAGVELVSTGSTAGRIADAGIPVTKVEELTGFPETLDGRVKTLHPRVHAGIL
ADTRKPEHLDQLTELGVEAFQLVVVNLYPFTQTVASGASPDECVEQIDIGGPSMVRAAAKNHPSVAVVVDAGDYDDVLAA
VRAGGFTLAQRTALAAKAFQHTAGYDVAVASWLTGVLAPSVADQPPSTPYPAWIGGSWERAAVLRYGENPHQSAALYTSN
DGSPGLAQAQQLHGKEMSYNNYTDADAAWRAAYDHEAPAVAIIKHANPCGIAIGADIAEAHRKAHACDPVSAFGGVIAAN
REVTVEMAEQVADIFTEVIVAPSYADGAVEVLQRKKNVRILIAEPPQRAGVELRPISGGALLQQRDILDAEGDDPANWTL
VAGTAADEQTLADLAFAWRACRAVKSNAILLASDGASVGVGMGQVNRVDAVQLAVRRAGDRAKGSVAASDAFFPFPDGPQ
QLIQAGVRAIVQPGGSVRDQETIDLAREAGVTLYLTGSRHFAH

Sequences:

>Translated_523_residues
MSERKPIRRALVSVYDKTGLVELATGLHAAGVELVSTGSTAGRIADAGIPVTKVEELTGFPETLDGRVKTLHPRVHAGIL
ADTRKPEHLDQLTELGVEAFQLVVVNLYPFTQTVASGASPDECVEQIDIGGPSMVRAAAKNHPSVAVVVDAGDYDDVLAA
VRAGGFTLAQRTALAAKAFQHTAGYDVAVASWLTGVLAPSVADQPPSTPYPAWIGGSWERAAVLRYGENPHQSAALYTSN
DGSPGLAQAQQLHGKEMSYNNYTDADAAWRAAYDHEAPAVAIIKHANPCGIAIGADIAEAHRKAHACDPVSAFGGVIAAN
REVTVEMAEQVADIFTEVIVAPSYADGAVEVLQRKKNVRILIAEPPQRAGVELRPISGGALLQQRDILDAEGDDPANWTL
VAGTAADEQTLADLAFAWRACRAVKSNAILLASDGASVGVGMGQVNRVDAVQLAVRRAGDRAKGSVAASDAFFPFPDGPQ
QLIQAGVRAIVQPGGSVRDQETIDLAREAGVTLYLTGSRHFAH
>Mature_522_residues
SERKPIRRALVSVYDKTGLVELATGLHAAGVELVSTGSTAGRIADAGIPVTKVEELTGFPETLDGRVKTLHPRVHAGILA
DTRKPEHLDQLTELGVEAFQLVVVNLYPFTQTVASGASPDECVEQIDIGGPSMVRAAAKNHPSVAVVVDAGDYDDVLAAV
RAGGFTLAQRTALAAKAFQHTAGYDVAVASWLTGVLAPSVADQPPSTPYPAWIGGSWERAAVLRYGENPHQSAALYTSND
GSPGLAQAQQLHGKEMSYNNYTDADAAWRAAYDHEAPAVAIIKHANPCGIAIGADIAEAHRKAHACDPVSAFGGVIAANR
EVTVEMAEQVADIFTEVIVAPSYADGAVEVLQRKKNVRILIAEPPQRAGVELRPISGGALLQQRDILDAEGDDPANWTLV
AGTAADEQTLADLAFAWRACRAVKSNAILLASDGASVGVGMGQVNRVDAVQLAVRRAGDRAKGSVAASDAFFPFPDGPQQ
LIQAGVRAIVQPGGSVRDQETIDLAREAGVTLYLTGSRHFAH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=501, Percent_Identity=35.7285429141717, Blast_Score=238, Evalue=9e-63,
Organism=Escherichia coli, GI1790439, Length=537, Percent_Identity=45.0651769087523, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71985564, Length=491, Percent_Identity=35.234215885947, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI71985574, Length=323, Percent_Identity=27.8637770897833, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=483, Percent_Identity=34.7826086956522, Blast_Score=233, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6323768, Length=496, Percent_Identity=35.6854838709677, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24649832, Length=493, Percent_Identity=36.3083164300203, Blast_Score=232, Evalue=5e-61,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54935; Mature: 54804

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERKPIRRALVSVYDKTGLVELATGLHAAGVELVSTGSTAGRIADAGIPVTKVEELTGF
CCCCCHHHHHHHHHHHCCCHHHHHHCCHHCCEEEEECCCCCCCCCCCCCCHHHHHHHCCC
PETLDGRVKTLHPRVHAGILADTRKPEHLDQLTELGVEAFQLVVVNLYPFTQTVASGASP
CHHHCCCEEEECCHHHHCCEECCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHCCCCH
DECVEQIDIGGPSMVRAAAKNHPSVAVVVDAGDYDDVLAAVRAGGFTLAQRTALAAKAFQ
HHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
HTAGYDVAVASWLTGVLAPSVADQPPSTPYPAWIGGSWERAAVLRYGENPHQSAALYTSN
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHEECCCCCCCCEEEEECC
DGSPGLAQAQQLHGKEMSYNNYTDADAAWRAAYDHEAPAVAIIKHANPCGIAIGADIAEA
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHH
HRKAHACDPVSAFGGVIAANREVTVEMAEQVADIFTEVIVAPSYADGAVEVLQRKKNVRI
HHHHCCCCCHHHHCCEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEE
LIAEPPQRAGVELRPISGGALLQQRDILDAEGDDPANWTLVAGTAADEQTLADLAFAWRA
EEECCCCCCCCEEEECCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
CRAVKSNAILLASDGASVGVGMGQVNRVDAVQLAVRRAGDRAKGSVAASDAFFPFPDGPQ
HHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHH
QLIQAGVRAIVQPGGSVRDQETIDLAREAGVTLYLTGSRHFAH
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCC
>Mature Secondary Structure 
SERKPIRRALVSVYDKTGLVELATGLHAAGVELVSTGSTAGRIADAGIPVTKVEELTGF
CCCCHHHHHHHHHHHCCCHHHHHHCCHHCCEEEEECCCCCCCCCCCCCCHHHHHHHCCC
PETLDGRVKTLHPRVHAGILADTRKPEHLDQLTELGVEAFQLVVVNLYPFTQTVASGASP
CHHHCCCEEEECCHHHHCCEECCCCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHCCCCH
DECVEQIDIGGPSMVRAAAKNHPSVAVVVDAGDYDDVLAAVRAGGFTLAQRTALAAKAFQ
HHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
HTAGYDVAVASWLTGVLAPSVADQPPSTPYPAWIGGSWERAAVLRYGENPHQSAALYTSN
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHEECCCCCCCCEEEEECC
DGSPGLAQAQQLHGKEMSYNNYTDADAAWRAAYDHEAPAVAIIKHANPCGIAIGADIAEA
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHH
HRKAHACDPVSAFGGVIAANREVTVEMAEQVADIFTEVIVAPSYADGAVEVLQRKKNVRI
HHHHCCCCCHHHHCCEEECCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEE
LIAEPPQRAGVELRPISGGALLQQRDILDAEGDDPANWTLVAGTAADEQTLADLAFAWRA
EEECCCCCCCCEEEECCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
CRAVKSNAILLASDGASVGVGMGQVNRVDAVQLAVRRAGDRAKGSVAASDAFFPFPDGPQ
HHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHH
QLIQAGVRAIVQPGGSVRDQETIDLAREAGVTLYLTGSRHFAH
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA