BacMap

Definition	Nocardia farcinica IFM 10152 chromosome, complete genome.
Accession	NC_006361
Length	6,021,225

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The map label for this gene is lcfB [H]

Identifier: 54026717

GI number: 54026717

Start: 4954116

End: 4955555

Strand: Reverse

Name: lcfB [H]

Synonym: nfa47430

Alternate gene names: 54026717

Gene position: 4955555-4954116 (Counterclockwise)

Preceding gene: 54026720

Following gene: 54026715

Centisome position: 82.3

GC content: 74.65

Gene sequence:

>1440_bases
ATGGCGTTCGGTGCACCCCTGCTGCTCAGTTCCCTGAATCCCGCCGCGGTCGCCGCGGGCGCCGACATCCCCGCCGCCGT
CACCATCGACGGCGTGACCCTCTCGCGCGGCGATCTGCTCGGCGCGGCGACCTCGGTGGCCGAACGGGTGGCGCGCGCGG
AGCGGGTGGCGGTGCTGGCCCGCCCGACGGTGCGCACCGTGCTCGCCGTGGTCGGCAGCCTGCTCGCCGGTGTCACGGTG
GTGCCGGTGCCGCCCGATTCCGGGGCGGCCGAACTCGCCCACATCCTGGCCGATTCCGGCGCACAGGCGTGGCTCGGCGA
CGCACCGGACGACACCGATCTGCCGGTGATCCCGGTCCGGGTGCACGCCCGCTCCTGGCACACCTATCCCGAACCCGACT
CCGCCGCACCGGCTTTCGTGCTCTACACCTCCGGCACCACCGGCCCGCCCAAGGGTGTGGTGCTCAGCCGTCGCGCCATC
GCCGCGGGGCTGGACGCGCTGGCCGAGGCGTGGGAGTGGACCGCGAACGACACGCTCGTGCACGGGCTTCCGCTGTTCCA
CGTGCACGGGCTGGTGCTCGGGGTGCTCGGCCCCCTGCGGGTGGGCAGCCCGCTGGTGCACACCGGCAAGCCGACCCCGC
GGGCCTACGCCGAGGCGAAGGGCACGCTGTACTTCGGGGTGCCGACGGTGTGGTCGCGCATCGTGGAGGATCCGGAATCG
GCCCGCGCGCTGCGGGATGCGCGCCTGCTGGTCTCGGGGTCGGCGCCGCTGCCGGTGCCGGTGTTCGAGCGGCTGCGCGA
ACTCACCGGCCACGCGCCGGTCGAGCGCTACGGCATGAGCGAGACCATGATCACCCTGTCCACCCGGGCCGACGGTGAAC
GCAGGCCCGGCTGGGTGGGCGTGCCGGTGCGCGGCGTGCAGACCCGCTTGCGGGACGAGGCCGGCGCGCCGGTGCCGCAC
GACGGCGAGAGCATCGGCGGCCTGCAGGTGCGCGGCCCGATGCTCTTCGACGGCTACCTGGGCCGCCCCGACGCCACCGC
CGCGAGCTGGACCGAGGACGGGTGGTTCAGCACCGGCGACGTCGCCGCCATCGACCCGGACGGATTCCACCGCATCGTCG
GCCGCGAGTCCGTCGACCTCATCAAGTCCGGCGGCTACCGCATCGGCGCGGGCGAGGTGGAGACCGCCCTGCTCGGCCAC
CCCGCGGTGGCGGAGGCGGCCGTGGTCGGCCTCGCCGACGACGACCTGGGCCAGCGGGTGGTCGCCTTCGTCGTCGCGCG
GGCCGGGCACGACCGGGACGACCTGCCGACGGCGCTGATCGGCCATGTGGCCGACCAGCTCTCGGTGCACAAACGCCCCC
GCGAGGTGCGGGTGGTGGACTCGCTGCCGCGCAACGCGATGGGCAAGGTTCAGAAGAAGGCCCTGGCCGCCGGTGGCTGA

Upstream 100 bases:

>100_bases
GTGTCGCAGGCGTGTCAACAGTCGCCCGCGGGCCTCGACCGATTCGTGACGCGACGGGGGAGACGGCGGCCGCCGCGGCC
CCGCGCAGTAGGTTGACCCC

Downstream 100 bases:

>100_bases
GCACCGGCGGGCGTGGACTCAGTTGTGGGCGTGCAGGTCCTCGTTGAGTTCGATGCCGGTGCCCTTGCGGTGCACCACCT
CGACCGCGCCCGTGGTGGAG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAFGAPLLLSSLNPAAVAAGADIPAAVTIDGVTLSRGDLLGAATSVAERVARAERVAVLARPTVRTVLAVVGSLLAGVTV
VPVPPDSGAAELAHILADSGAQAWLGDAPDDTDLPVIPVRVHARSWHTYPEPDSAAPAFVLYTSGTTGPPKGVVLSRRAI
AAGLDALAEAWEWTANDTLVHGLPLFHVHGLVLGVLGPLRVGSPLVHTGKPTPRAYAEAKGTLYFGVPTVWSRIVEDPES
ARALRDARLLVSGSAPLPVPVFERLRELTGHAPVERYGMSETMITLSTRADGERRPGWVGVPVRGVQTRLRDEAGAPVPH
DGESIGGLQVRGPMLFDGYLGRPDATAASWTEDGWFSTGDVAAIDPDGFHRIVGRESVDLIKSGGYRIGAGEVETALLGH
PAVAEAAVVGLADDDLGQRVVAFVVARAGHDRDDLPTALIGHVADQLSVHKRPREVRVVDSLPRNAMGKVQKKALAAGG

Sequences:

>Translated_479_residues
MAFGAPLLLSSLNPAAVAAGADIPAAVTIDGVTLSRGDLLGAATSVAERVARAERVAVLARPTVRTVLAVVGSLLAGVTV
VPVPPDSGAAELAHILADSGAQAWLGDAPDDTDLPVIPVRVHARSWHTYPEPDSAAPAFVLYTSGTTGPPKGVVLSRRAI
AAGLDALAEAWEWTANDTLVHGLPLFHVHGLVLGVLGPLRVGSPLVHTGKPTPRAYAEAKGTLYFGVPTVWSRIVEDPES
ARALRDARLLVSGSAPLPVPVFERLRELTGHAPVERYGMSETMITLSTRADGERRPGWVGVPVRGVQTRLRDEAGAPVPH
DGESIGGLQVRGPMLFDGYLGRPDATAASWTEDGWFSTGDVAAIDPDGFHRIVGRESVDLIKSGGYRIGAGEVETALLGH
PAVAEAAVVGLADDDLGQRVVAFVVARAGHDRDDLPTALIGHVADQLSVHKRPREVRVVDSLPRNAMGKVQKKALAAGG
>Mature_478_residues
AFGAPLLLSSLNPAAVAAGADIPAAVTIDGVTLSRGDLLGAATSVAERVARAERVAVLARPTVRTVLAVVGSLLAGVTVV
PVPPDSGAAELAHILADSGAQAWLGDAPDDTDLPVIPVRVHARSWHTYPEPDSAAPAFVLYTSGTTGPPKGVVLSRRAIA
AGLDALAEAWEWTANDTLVHGLPLFHVHGLVLGVLGPLRVGSPLVHTGKPTPRAYAEAKGTLYFGVPTVWSRIVEDPESA
RALRDARLLVSGSAPLPVPVFERLRELTGHAPVERYGMSETMITLSTRADGERRPGWVGVPVRGVQTRLRDEAGAPVPHD
GESIGGLQVRGPMLFDGYLGRPDATAASWTEDGWFSTGDVAAIDPDGFHRIVGRESVDLIKSGGYRIGAGEVETALLGHP
AVAEAAVVGLADDDLGQRVVAFVVARAGHDRDDLPTALIGHVADQLSVHKRPREVRVVDSLPRNAMGKVQKKALAAGG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=479, Percent_Identity=35.2818371607516, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI187761343, Length=479, Percent_Identity=35.2818371607516, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI157311624, Length=477, Percent_Identity=27.8825995807128, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI157311622, Length=477, Percent_Identity=27.8825995807128, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI115511026, Length=386, Percent_Identity=31.3471502590674, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI122937307, Length=362, Percent_Identity=30.6629834254144, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI42544132, Length=367, Percent_Identity=29.1553133514986, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI58082049, Length=477, Percent_Identity=27.6729559748428, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI38505220, Length=367, Percent_Identity=30.2452316076294, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI156151445, Length=354, Percent_Identity=28.5310734463277, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI40807491, Length=347, Percent_Identity=27.6657060518732, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28416953, Length=377, Percent_Identity=28.6472148541114, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI57165410, Length=345, Percent_Identity=26.3768115942029, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI57165412, Length=345, Percent_Identity=26.3768115942029, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI13375727, Length=379, Percent_Identity=27.7044854881267, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI8923896, Length=363, Percent_Identity=24.7933884297521, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI253970489, Length=363, Percent_Identity=24.7933884297521, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788107, Length=362, Percent_Identity=30.939226519337, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI145693145, Length=345, Percent_Identity=31.304347826087, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1786810, Length=376, Percent_Identity=30.0531914893617, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI221142682, Length=352, Percent_Identity=27.5568181818182, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790505, Length=374, Percent_Identity=28.6096256684492, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786801, Length=371, Percent_Identity=29.3800539083558, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786529, Length=389, Percent_Identity=29.305912596401, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788595, Length=427, Percent_Identity=26.6978922716628, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=494, Percent_Identity=32.1862348178138, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32563687, Length=450, Percent_Identity=32.2222222222222, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17531443, Length=356, Percent_Identity=28.9325842696629, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=390, Percent_Identity=29.7435897435897, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=375, Percent_Identity=29.6, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=375, Percent_Identity=29.6, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=375, Percent_Identity=29.6, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=356, Percent_Identity=29.4943820224719, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=369, Percent_Identity=27.6422764227642, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=366, Percent_Identity=26.775956284153, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=368, Percent_Identity=26.3586956521739, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556552, Length=358, Percent_Identity=28.2122905027933, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=445, Percent_Identity=24.7191011235955, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17553312, Length=352, Percent_Identity=28.125, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17510401, Length=182, Percent_Identity=29.6703296703297, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17541856, Length=308, Percent_Identity=26.9480519480519, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=375, Percent_Identity=27.4666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982997, Length=375, Percent_Identity=27.4666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=351, Percent_Identity=31.6239316239316, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319264, Length=381, Percent_Identity=28.3464566929134, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323182, Length=388, Percent_Identity=27.0618556701031, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320852, Length=183, Percent_Identity=27.8688524590164, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355181, Length=367, Percent_Identity=30.2452316076294, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI18859661, Length=459, Percent_Identity=27.0152505446623, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24581924, Length=355, Percent_Identity=28.4507042253521, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI221329755, Length=421, Percent_Identity=25.8907363420428, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922652, Length=354, Percent_Identity=28.2485875706215, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24656500, Length=479, Percent_Identity=25.4697286012526, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653035, Length=344, Percent_Identity=27.0348837209302, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648260, Length=490, Percent_Identity=24.6938775510204, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648257, Length=368, Percent_Identity=26.0869565217391, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648255, Length=487, Percent_Identity=22.3819301848049, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI161076582, Length=350, Percent_Identity=26.2857142857143, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356947, Length=349, Percent_Identity=26.3610315186246, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648253, Length=487, Percent_Identity=22.17659137577, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281366413, Length=352, Percent_Identity=26.4204545454545, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24666501, Length=352, Percent_Identity=26.4204545454545, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24666497, Length=352, Percent_Identity=26.4204545454545, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356441, Length=354, Percent_Identity=25.9887005649718, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667959, Length=373, Percent_Identity=25.7372654155496, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472339, Length=373, Percent_Identity=25.7372654155496, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20130357, Length=350, Percent_Identity=26.5714285714286, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24667955, Length=373, Percent_Identity=25.7372654155496, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21358303, Length=316, Percent_Identity=21.8354430379747, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI281365686, Length=298, Percent_Identity=22.4832214765101, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 49956; Mature: 49825

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFGAPLLLSSLNPAAVAAGADIPAAVTIDGVTLSRGDLLGAATSVAERVARAERVAVLA
CCCCCCHHHHCCCCHHEECCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHEEE
RPTVRTVLAVVGSLLAGVTVVPVPPDSGAAELAHILADSGAQAWLGDAPDDTDLPVIPVR
CCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
VHARSWHTYPEPDSAAPAFVLYTSGTTGPPKGVVLSRRAIAAGLDALAEAWEWTANDTLV
EEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEE
HGLPLFHVHGLVLGVLGPLRVGSPLVHTGKPTPRAYAEAKGTLYFGVPTVWSRIVEDPES
ECCHHHHHHHHHHHHHCHHHCCCCCEECCCCCCHHHHCCCCEEEECHHHHHHHHHCCHHH
ARALRDARLLVSGSAPLPVPVFERLRELTGHAPVERYGMSETMITLSTRADGERRPGWVG
HHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCCCCCEEC
VPVRGVQTRLRDEAGAPVPHDGESIGGLQVRGPMLFDGYLGRPDATAASWTEDGWFSTGD
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCC
VAAIDPDGFHRIVGRESVDLIKSGGYRIGAGEVETALLGHPAVAEAAVVGLADDDLGQRV
EEEECCCHHHHHHCCCHHHHHHCCCEEECCCCHHHHHHCCCHHHHHEEEECCCCHHHHHH
VAFVVARAGHDRDDLPTALIGHVADQLSVHKRPREVRVVDSLPRNAMGKVQKKALAAGG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFGAPLLLSSLNPAAVAAGADIPAAVTIDGVTLSRGDLLGAATSVAERVARAERVAVLA
CCCCCHHHHCCCCHHEECCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHHEEE
RPTVRTVLAVVGSLLAGVTVVPVPPDSGAAELAHILADSGAQAWLGDAPDDTDLPVIPVR
CCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
VHARSWHTYPEPDSAAPAFVLYTSGTTGPPKGVVLSRRAIAAGLDALAEAWEWTANDTLV
EEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEE
HGLPLFHVHGLVLGVLGPLRVGSPLVHTGKPTPRAYAEAKGTLYFGVPTVWSRIVEDPES
ECCHHHHHHHHHHHHHCHHHCCCCCEECCCCCCHHHHCCCCEEEECHHHHHHHHHCCHHH
ARALRDARLLVSGSAPLPVPVFERLRELTGHAPVERYGMSETMITLSTRADGERRPGWVG
HHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCCCCCEEC
VPVRGVQTRLRDEAGAPVPHDGESIGGLQVRGPMLFDGYLGRPDATAASWTEDGWFSTGD
CCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCC
VAAIDPDGFHRIVGRESVDLIKSGGYRIGAGEVETALLGHPAVAEAAVVGLADDDLGQRV
EEEECCCHHHHHHCCCHHHHHHCCCEEECCCCHHHHHHCCCHHHHHEEEECCCCHHHHHH
VAFVVARAGHDRDDLPTALIGHVADQLSVHKRPREVRVVDSLPRNAMGKVQKKALAAGG
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]