Click here to switch to the map view.

The map label for this gene is phbC [H]

Identifier: 54026545

GI number: 54026545

Start: 4754324

End: 4755409

Strand: Reverse

Name: phbC [H]

Synonym: nfa45720

Alternate gene names: 54026545

Gene position: 4755409-4754324 (Counterclockwise)

Preceding gene: 54026551

Following gene: 54026544

Centisome position: 78.98

GC content: 73.76

Gene sequence:

>1086_bases
GTGAGCCTCGCAGACACTCTCACCCTCGCCGCCCGCAACGCCTGGGCGCTGACCTTCGGTCCCGGGGTGGAAGCACCCGA
ACCGACCCGGTCCACCGTCCTCTGGGACGCCGCACACCGGGAGCTGCGCCGTTTCGAGCGCGACGAAGCCCGCGACGGCG
CCGCGGCCGAGGGCGCCGACCCGGTCCTGCTGGTGCCGCCGCTGGCCGCCCCCGCCTCCTGCTTCGACCTGCGCCCCGAC
CAGAGCCTGGCGCGCTTCCTGCTCGGCACCGGCCGCACGCCGTACGTGGTCGACTACGGCGAGATCACCTTCGCCGACCG
CCGGATGGGTTTCGAGGACTGGATCAACGACATCCTGCCCGAGGCCGTGCTGCGCACCAGCGCCGATCGGGACGGCGCCG
CCGTCGACCTGGTGGGCTGGTCGCTCGGCGGCACCCTGGCCCTGCTCACCGCCGCCGCGCATCCGCAGCTGCCGATCGGC
TCGATCACCGCGGTCGGCTCGCCGCTCGACTACGACCGGATGACCGGCATGCCGCAGGTGCGCGCGGTCGCCAAGCTGGA
CGGCGGACTGGCCGTCTCCACCGCGGTCCGCGCCGCGGGCGGCATCCCCGCGCCGCTGACCCGGGCCGCCTACCGGGTGA
CCGCGTGGAACCGGGAACTGACCCGGCCGCTGTTCGTCGCGAGCAACATCGCCCGCACCGAGGCACTGGCCAAGATGGAG
TCCATCGACCGCTTCATGGCGCAGATGCCCGGCTATCCCGGCCGGTTCTACGGGCAGCTGTGGGGCAGGCTGATCCTGAA
CAACGACATCGGCCGCGGCGTGCTGCGCCTGGGCGGACGCGAGATCGCGCTGGCGGCGGTGACGGCGCCGGTGCTGCTGG
TCGGCGGGCCCGCCGACGTGATCACCCCGGCACCGGCGGTCGAAGCGGGCACCCGCACCCTCACCGGCGCGGCGTTCGTG
CGCTACGAGACGGCGCCGGGCAGCCACCTGGGCATCCTCACCGGCGAAACCGCCCGTGAGACCACCTGGACCTACCTGGA
CGAGTTCCTCACCGAGGCGGCCGCCGTGCGCGAATCGGTGTCCTGA

Upstream 100 bases:

>100_bases
CGTTTCGGCCGCGCCGCGCGACCACGGCGGCCGGTCCGCGACACCGCCGGGACGGGGGCTTGCTGTAACCGTCGGTAGCG
GGTTGTGAGAGATTCTTGCC

Downstream 100 bases:

>100_bases
CCCGCGCGGACCTTCGCGCGCCGCTACTCTTGGCGACATGGGAGCACAGTCATCCGCAGCGGTCGGTGTCTCGTCGTCGC
AGCAGTCCGCGAGTCCGGCC

Product: putative hydrolase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSLADTLTLAARNAWALTFGPGVEAPEPTRSTVLWDAAHRELRRFERDEARDGAAAEGADPVLLVPPLAAPASCFDLRPD
QSLARFLLGTGRTPYVVDYGEITFADRRMGFEDWINDILPEAVLRTSADRDGAAVDLVGWSLGGTLALLTAAAHPQLPIG
SITAVGSPLDYDRMTGMPQVRAVAKLDGGLAVSTAVRAAGGIPAPLTRAAYRVTAWNRELTRPLFVASNIARTEALAKME
SIDRFMAQMPGYPGRFYGQLWGRLILNNDIGRGVLRLGGREIALAAVTAPVLLVGGPADVITPAPAVEAGTRTLTGAAFV
RYETAPGSHLGILTGETARETTWTYLDEFLTEAAAVRESVS

Sequences:

>Translated_361_residues
MSLADTLTLAARNAWALTFGPGVEAPEPTRSTVLWDAAHRELRRFERDEARDGAAAEGADPVLLVPPLAAPASCFDLRPD
QSLARFLLGTGRTPYVVDYGEITFADRRMGFEDWINDILPEAVLRTSADRDGAAVDLVGWSLGGTLALLTAAAHPQLPIG
SITAVGSPLDYDRMTGMPQVRAVAKLDGGLAVSTAVRAAGGIPAPLTRAAYRVTAWNRELTRPLFVASNIARTEALAKME
SIDRFMAQMPGYPGRFYGQLWGRLILNNDIGRGVLRLGGREIALAAVTAPVLLVGGPADVITPAPAVEAGTRTLTGAAFV
RYETAPGSHLGILTGETARETTWTYLDEFLTEAAAVRESVS
>Mature_360_residues
SLADTLTLAARNAWALTFGPGVEAPEPTRSTVLWDAAHRELRRFERDEARDGAAAEGADPVLLVPPLAAPASCFDLRPDQ
SLARFLLGTGRTPYVVDYGEITFADRRMGFEDWINDILPEAVLRTSADRDGAAVDLVGWSLGGTLALLTAAAHPQLPIGS
ITAVGSPLDYDRMTGMPQVRAVAKLDGGLAVSTAVRAAGGIPAPLTRAAYRVTAWNRELTRPLFVASNIARTEALAKMES
IDRFMAQMPGYPGRFYGQLWGRLILNNDIGRGVLRLGGREIALAAVTAPVLLVGGPADVITPAPAVEAGTRTLTGAAFVR
YETAPGSHLGILTGETARETTWTYLDEFLTEAAAVRESVS

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010125 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 38421; Mature: 38290

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLADTLTLAARNAWALTFGPGVEAPEPTRSTVLWDAAHRELRRFERDEARDGAAAEGAD
CCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCC
PVLLVPPLAAPASCFDLRPDQSLARFLLGTGRTPYVVDYGEITFADRRMGFEDWINDILP
CEEEECCCCCCCHHHCCCCCHHHHHHHHCCCCCCEEEECCCEEEHHHHCCHHHHHHHHHH
EAVLRTSADRDGAAVDLVGWSLGGTLALLTAAAHPQLPIGSITAVGSPLDYDRMTGMPQV
HHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEECCCCCCHHHHCCCHHH
RAVAKLDGGLAVSTAVRAAGGIPAPLTRAAYRVTAWNRELTRPLFVASNIARTEALAKME
HHHHHHCCCCHHHHHHHHCCCCCCCHHHHHEEEEECCHHHCCHHHHHHHHHHHHHHHHHH
SIDRFMAQMPGYPGRFYGQLWGRLILNNDIGRGVLRLGGREIALAAVTAPVLLVGGPADV
HHHHHHHHCCCCCCHHHHHHHHHEEECCCCCCHHHHHCCCEEEEEEEEECEEEECCCHHH
ITPAPAVEAGTRTLTGAAFVRYETAPGSHLGILTGETARETTWTYLDEFLTEAAAVRESV
CCCCCCCCCCCCEEECEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure 
SLADTLTLAARNAWALTFGPGVEAPEPTRSTVLWDAAHRELRRFERDEARDGAAAEGAD
CHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCC
PVLLVPPLAAPASCFDLRPDQSLARFLLGTGRTPYVVDYGEITFADRRMGFEDWINDILP
CEEEECCCCCCCHHHCCCCCHHHHHHHHCCCCCCEEEECCCEEEHHHHCCHHHHHHHHHH
EAVLRTSADRDGAAVDLVGWSLGGTLALLTAAAHPQLPIGSITAVGSPLDYDRMTGMPQV
HHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCEECCCCCCHHHHCCCHHH
RAVAKLDGGLAVSTAVRAAGGIPAPLTRAAYRVTAWNRELTRPLFVASNIARTEALAKME
HHHHHHCCCCHHHHHHHHCCCCCCCHHHHHEEEEECCHHHCCHHHHHHHHHHHHHHHHHH
SIDRFMAQMPGYPGRFYGQLWGRLILNNDIGRGVLRLGGREIALAAVTAPVLLVGGPADV
HHHHHHHHCCCCCCHHHHHHHHHEEECCCCCCHHHHHCCCEEEEEEEEECEEEECCCHHH
ITPAPAVEAGTRTLTGAAFVRYETAPGSHLGILTGETARETTWTYLDEFLTEAAAVRESV
CCCCCCCCCCCCEEECEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7763384 [H]