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The map label for this gene is narK [H]

Identifier: 54026535

GI number: 54026535

Start: 4743343

End: 4744812

Strand: Reverse

Name: narK [H]

Synonym: nfa45620

Alternate gene names: 54026535

Gene position: 4744812-4743343 (Counterclockwise)

Preceding gene: 54026536

Following gene: 54026534

Centisome position: 78.8

GC content: 68.3

Gene sequence:

>1470_bases
TTGTTGAGCACGCTGACGCGTAACCGCAACATCGAGCACTGGGATGCCGAAGACGTCGAGGCATGGGAGGCCGGTGGCAA
GCACATCGCCCGGCGCAACCTGATCTGGTCGGTCTTCGCCGAGCACGTCGGATTCTCGATCTGGTCGATCTGGTCGGTGA
TGGTGCTGTTCATGCCCGCCGAGAAGTACGGCATCGACGCGGCGGGTAAGTTCTTCCTGATGGCGGTGCCGACCCTGGTC
GGTGCGGTGCTGCGCATTCCCTACACCGTCGCCACCGCCCGCTTCGGTGGCCGCAACTGGACCGTCTTCTCGGCCTTGCT
GCTGCTGGTGCCCACGCTGCTGACGCTGTACTTCATCAACCAGCCCGGCACCTCGTACACCACCTTCCTGGTGGTGGCGG
CCTTCGCCGGTGTCGGTGGCGGCAACTTCGCCTCGTCGATGACCAACATCAACGCCTTCTACCCGCAGCGGCTCAAGGGC
TGGGCGCTCGGGCTGAACGCCGGCGGCGGCAACATCGGCGTGCCGGTCATCCAGCTGATCGGCCTGCTGGTCATCGCCAC
CCTCGGCAACGAGTACGCCAGCCTGATCTGCGCGATCTACCTGGTGCTGATCGCGATCACCGGCCTGGGCGCCGCGCTGT
ACATGGACAACCTGCGCAACCAGAAGGCGGACCTGTCCGACATGGTGACCGCGCTGAAAGTGCCGCAGTCCTGGGCGATC
GCGTTCCTCTACATCGGCACGTTCGGATCGTTCATCGGCTACAGCTTCGCCTTCGGCCAGATCCTGCAGATCAGCTTCAA
GGCCGGTGGCGACACCGCCGCGCAGGCCGCGCTGCACGCCGCCCAGATCGCCTTCATCGGCCCGCTGCTCGGCTCGCTGG
CCCGCCCCTACGGCGGCAAGTGGGCCGACCGGATCGGCGGCAGCCGCGTCGCGCTGGTCACCTTCGGCGCGATGATGGCC
GCGGCCGCGCTGGTGATCGGGGCCAGCACCGTGGCCGACGGCAACGGCGGCGTCGCAGGCGGCGCGGTGATGGCGGTGCT
CGTGATCGGTTTCGTCGCCCTGTTCGTGCTCTCGGGCATCGGCAACGGCGCGGTCACCAAGATCATCCCGTCGGTGTTCG
ACGCCAAGTCGCGCAGCCTCGACCTGAGCCCCGCCGATCGGGCCGCCTGGTCGCAGAACACCTCCGGCGCGCTGATCGGT
TTCGTCGGCGCCATCGGAGCGCTGGGCGGCGTCGGCATCAACCTGGTCCTGCGCTCCTCCTACGCCTCGACCAACTCGGC
CACCACCGCGTTCTGGGTCTTCCTGGCCTTCTACGTGGTGTGCGCCGCGGTCACCTGGCTGGTCTTCCTGCGCCGTCCGA
GCCTGCGCTCGGTGGCCGACGACGATGTGGTCGTCGAGGCCGAGGCCCTCGTTCGCCGCGCCGAACAGGCCGTTCCCGCC
ACCTCGTCCGTCCCGTCGGCCCGCGCCTGA

Upstream 100 bases:

>100_bases
GCCGTCGCGGGTGAGCAACCGTTGATTCGGCCGTCATGTACGGCAGCATTTCGGCAATACCCGCTCCCTACCGTTGGGGC
CAACCGATTTTGGGAGGCCC

Downstream 100 bases:

>100_bases
CGCCCGCATCCATTCCTGGGAGGTCCACATGAACCCCGCAACCACCGAGCGCAAGACCGTGGTGGTCGTCGGCCACGGCA
TGGTCGGGCACCGCTTCGTC

Product: putative nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MLSTLTRNRNIEHWDAEDVEAWEAGGKHIARRNLIWSVFAEHVGFSIWSIWSVMVLFMPAEKYGIDAAGKFFLMAVPTLV
GAVLRIPYTVATARFGGRNWTVFSALLLLVPTLLTLYFINQPGTSYTTFLVVAAFAGVGGGNFASSMTNINAFYPQRLKG
WALGLNAGGGNIGVPVIQLIGLLVIATLGNEYASLICAIYLVLIAITGLGAALYMDNLRNQKADLSDMVTALKVPQSWAI
AFLYIGTFGSFIGYSFAFGQILQISFKAGGDTAAQAALHAAQIAFIGPLLGSLARPYGGKWADRIGGSRVALVTFGAMMA
AAALVIGASTVADGNGGVAGGAVMAVLVIGFVALFVLSGIGNGAVTKIIPSVFDAKSRSLDLSPADRAAWSQNTSGALIG
FVGAIGALGGVGINLVLRSSYASTNSATTAFWVFLAFYVVCAAVTWLVFLRRPSLRSVADDDVVVEAEALVRRAEQAVPA
TSSVPSARA

Sequences:

>Translated_489_residues
MLSTLTRNRNIEHWDAEDVEAWEAGGKHIARRNLIWSVFAEHVGFSIWSIWSVMVLFMPAEKYGIDAAGKFFLMAVPTLV
GAVLRIPYTVATARFGGRNWTVFSALLLLVPTLLTLYFINQPGTSYTTFLVVAAFAGVGGGNFASSMTNINAFYPQRLKG
WALGLNAGGGNIGVPVIQLIGLLVIATLGNEYASLICAIYLVLIAITGLGAALYMDNLRNQKADLSDMVTALKVPQSWAI
AFLYIGTFGSFIGYSFAFGQILQISFKAGGDTAAQAALHAAQIAFIGPLLGSLARPYGGKWADRIGGSRVALVTFGAMMA
AAALVIGASTVADGNGGVAGGAVMAVLVIGFVALFVLSGIGNGAVTKIIPSVFDAKSRSLDLSPADRAAWSQNTSGALIG
FVGAIGALGGVGINLVLRSSYASTNSATTAFWVFLAFYVVCAAVTWLVFLRRPSLRSVADDDVVVEAEALVRRAEQAVPA
TSSVPSARA
>Mature_489_residues
MLSTLTRNRNIEHWDAEDVEAWEAGGKHIARRNLIWSVFAEHVGFSIWSIWSVMVLFMPAEKYGIDAAGKFFLMAVPTLV
GAVLRIPYTVATARFGGRNWTVFSALLLLVPTLLTLYFINQPGTSYTTFLVVAAFAGVGGGNFASSMTNINAFYPQRLKG
WALGLNAGGGNIGVPVIQLIGLLVIATLGNEYASLICAIYLVLIAITGLGAALYMDNLRNQKADLSDMVTALKVPQSWAI
AFLYIGTFGSFIGYSFAFGQILQISFKAGGDTAAQAALHAAQIAFIGPLLGSLARPYGGKWADRIGGSRVALVTFGAMMA
AAALVIGASTVADGNGGVAGGAVMAVLVIGFVALFVLSGIGNGAVTKIIPSVFDAKSRSLDLSPADRAAWSQNTSGALIG
FVGAIGALGGVGINLVLRSSYASTNSATTAFWVFLAFYVVCAAVTWLVFLRRPSLRSVADDDVVVEAEALVRRAEQAVPA
TSSVPSARA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=463, Percent_Identity=34.9892008639309, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1787743, Length=461, Percent_Identity=34.70715835141, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51283; Mature: 51283

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTLTRNRNIEHWDAEDVEAWEAGGKHIARRNLIWSVFAEHVGFSIWSIWSVMVLFMPA
CCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
EKYGIDAAGKFFLMAVPTLVGAVLRIPYTVATARFGGRNWTVFSALLLLVPTLLTLYFIN
HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
QPGTSYTTFLVVAAFAGVGGGNFASSMTNINAFYPQRLKGWALGLNAGGGNIGVPVIQLI
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHCHHHCHHHHCCEEEEEECCCCCCCHHHHHHH
GLLVIATLGNEYASLICAIYLVLIAITGLGAALYMDNLRNQKADLSDMVTALKVPQSWAI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHH
AFLYIGTFGSFIGYSFAFGQILQISFKAGGDTAAQAALHAAQIAFIGPLLGSLARPYGGK
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WADRIGGSRVALVTFGAMMAAAALVIGASTVADGNGGVAGGAVMAVLVIGFVALFVLSGI
HHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GNGAVTKIIPSVFDAKSRSLDLSPADRAAWSQNTSGALIGFVGAIGALGGVGINLVLRSS
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
YASTNSATTAFWVFLAFYVVCAAVTWLVFLRRPSLRSVADDDVVVEAEALVRRAEQAVPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCC
TSSVPSARA
CCCCCCCCC
>Mature Secondary Structure
MLSTLTRNRNIEHWDAEDVEAWEAGGKHIARRNLIWSVFAEHVGFSIWSIWSVMVLFMPA
CCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
EKYGIDAAGKFFLMAVPTLVGAVLRIPYTVATARFGGRNWTVFSALLLLVPTLLTLYFIN
HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
QPGTSYTTFLVVAAFAGVGGGNFASSMTNINAFYPQRLKGWALGLNAGGGNIGVPVIQLI
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHCHHHCHHHHCCEEEEEECCCCCCCHHHHHHH
GLLVIATLGNEYASLICAIYLVLIAITGLGAALYMDNLRNQKADLSDMVTALKVPQSWAI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHH
AFLYIGTFGSFIGYSFAFGQILQISFKAGGDTAAQAALHAAQIAFIGPLLGSLARPYGGK
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WADRIGGSRVALVTFGAMMAAAALVIGASTVADGNGGVAGGAVMAVLVIGFVALFVLSGI
HHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GNGAVTKIIPSVFDAKSRSLDLSPADRAAWSQNTSGALIGFVGAIGALGGVGINLVLRSS
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
YASTNSATTAFWVFLAFYVVCAAVTWLVFLRRPSLRSVADDDVVVEAEALVRRAEQAVPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCC
TSSVPSARA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]